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pH Indicators Product Specification

Catelog Number :   
    51009
Product Name :   
     BCECF, AM ester, 1mg/mL in anhydrous DMSO

Description:

See information for #51011

Chemical Structure of 51009

Reference:

 

 
Catelog Number :   
    51010
Product Name :   
     BCECF free acid (2',7' -bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein)

Description:

BCECF is the most widely used fluorescent pH sensor. Because its pKa (6.97) is close to physiological pH, BCECF can detect cytosolic pH change with high sensitivity. At low pH, the dye is weakly fluorescent but becomes more fluorescent with increasing pH. The excitation spectrum of the dye undergoes a slight shift during pH change, while the wavelength of the emission maximum remains unchanged. The pH is determined ratiometrically by the relative fluorescent intensities at 535 nm when the dye is excited at 439 nm and 505 nm respectively. BCECF acid is membrane-impermeant and therefore can be loaded into cells via microinjection or scrape loading (See #51011 ).
  • ex\em = 508nm\530nm.
  • Yellow orange solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • Mwt: 520.5
  • [85138-49-4]

Chemical Structure of 51010

Reference:

Kolber, M.A., et al. J. Immunol. Meth. 108, 255(1988); Carpenter, L., et al. Biochem. J. 304, 751(1994);
Seller, J.R. et al. J. Immunol. Meth. 172, 255(1994).
Catelog Number :   
    51011
Product Name :   
     BCECF, AM ester

Description:

BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells. For more information on BCECF, see #51010.
  • White solid soluble in DMSO and DMF
  • Store at -20°C and protect from light, especially in solution
  • Mwt: ~615
  • [117464-70-7]

Chemical Structure of 51011

Reference:

1) Ho, A.K., et al. Am. J. Physiol. 261, C642(1991); 2) Futsaetner, C.M., et al. Can. J. Microbiol. 39, 180(1993); 3) Shepherd, R.M., et al. Am. J. Physiol. 262, C182(1992); 4) Hayasi, H., et al. Am. J. Physiol. 262, C628(1992); 5) Weiner, I.D. and Hamm, L.L. Am. J. Physiol. 256, F957(1989).
Catelog Number :   
    51011-1
Product Name :   
     BCECF, AM ester

Description:

This special packaging size is convenient for high throughput drug screening. See information for #51011

Chemical Structure of 51011-1

Reference:
Catelog Number :   
    51012
Product Name :   
     BCECF, AM ester

Description:

See information for #51011

Chemical Structure of 51012

Reference:
Catelog Number :   
    51013
Product Name :   
     5-(and-6)-Carboxyfluorescein (5-(and-6)-FAM, mixed isomer)

Description:

Carboxyfluorescein has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
  • ex\em (pH 9) = 492nm\514nm.
  • e (pH 9) = 82,000.
  • Yellow orange solid soluble in pH>6 water.
  • Store at 4°C. Protect from light, especially when in solution.
  • C21H12O7.
  • Mwt: 376.
  • [72088-94-9]

Chemical Structure of 51013

Reference:

Weinstein, J.N., et al. Methods Enzymol. 128, 65(1986)
Catelog Number :   
    51014
Product Name :   
     5(6)-CFDA (5-(and-6)-Carboxyfluorescein diacetate)

Description:

5(6)-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (#51013).
  • Off-white solid soluble in DMSO
  • Store at 4°C
  • C25H16O9.
  • Mwt: 460
  • [124387-19-5]

Chemical Structure of 51014

Reference:

1)Boitano, S., et al. J. Cell Sci. 98, 343(1991); 2)Goodall, H., et al. Nature 295, 524(1982); 3)Hansson, Y., et al. J. Immunol. Meth. 100, 261(1987); 4)Bruning, J.W., et al. J. Immunol. Meth. 33, 33(1980).
Catelog Number :   
    51015
Product Name :   
     5-(and-6)-carboxy-2',7'-dichlorofluorescein

Description:

5-(and-6)-carboxy-2',7'-dichlorofluorescein is similar to carboxyfluorescein ( 51013)but has lower pKa, It is useful for detecting pH in more acidic environment. Also see 51016
  • pKa = 4.8.
  • ex\em = 504nm\529nm.
  • e= 90,000.
  • Orange solid soluble in DMSO and pH>6 buffers.
  • Store at 4°C. Protect from light, especially in solution.
  • C21H10Cl2O7.
  • Mwt: 445.
  • [111843-78-8]

Chemical Structure of 51015

Reference:

Bramhall, J., et al. Biochemistry 26, 6330(1987);
Knight, C.G., et al. Biochim. Biophys. Acta 985, 75(1989);
Nedergaard, M. et al. Anal. Biochem. 187, 109(1990).
Catelog Number :   
    51016
Product Name :   
     5-(&6)-carboxy-2',7'-dichlorofluorescein diacetate

Description:

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)- carboxy-2',7'-dichlorofluorescein (See #51015) by intracellular esterases.
  • White solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • C25H14Cl2O9.
  • Mwt: 529
  • [127770-45-0]

Chemical Structure of 51016

Reference:

1) J. Cell Biol. 111, 877(1990); 2) Biochemistry 26, 6330(1987); 3) Biochim. Biophys. Acta 985, 75(1989); 4) Anal. Biochem. 187, 109(1990).

 
Catelog Number :   
    51017
Product Name :   
     5-carboxy-2',7'-dichlorofluorescein (single isomer)

Description:

This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label. Carboxy-2',7'-dichlorofluorescein is significantly more photo-stable than carboxyfluorescein.
  • ex\em= 504/529 nm (pH 8)
  • e = 90,000
  • Yellow solid soluble in DMSO, DMF or basic water (pH >7).
  • Store at room temperature and protect from light.
  • C21H10Cl2O7
  • Mwt = 445.

Chemical Structure of 51017

Reference:
Catelog Number :   
    51018
Product Name :   
     5-CFDA (5-Carboxyfluorescein diacetate, single isomer)

Description:

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein.
  • Off-white solid soluble in DMSO
  • Store at 4°C
  • C25H16O9.
  • Mwt: 460
  • [79955-27-4]

Chemical Structure of 51018

Reference:

1)Boitano, S., et al. J. Cell Sci. 98, 343(1991); 2)Goodall, H., et al. Nature 295, 524(1982); 3)Hansson, Y., et al. J. Immunol. Meth. 100, 261(1987); 4)Bruning, J.W., et al. J. Immunol. Meth. 33, 33(1980).
Catelog Number :   
    51019
Product Name :   
     5-Carboxyfluorescein (5-FAM, single isomer)

Description:

Free acid form of 5-carboxyfluorescein single isomer. See Figure 7.4 for spectra.
  • ex\em(pH 9) = 492/514nm
  • Yellow orange solid soluble in pH>6 water
  • Store at 4°C and protect from light, especially in solution
  • C21H12O7
  • Mwt: 376.3

Chemical Structure of 51019

Reference:
Catelog Number :   
    51021
Product Name :   
     6-CFDA (6-Carboxyfluorescein diacetate, single isomer)

Description:

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.
  • Off-white solid soluble in DMSO
  • Store at 4°C
  • C25H16O9
  • Mwt: 460

Chemical Structure of 51021

Reference:

1) Boitano, S., et al. J. Cell Sci. 98, 343(1991); 2) Goodall, H., et al. Nature 295, 524(1982); 3) Hansson, Y., et al. J. Immunol. Meth. 100, 261(1987); 4) Bruning, J.W., et al. J. Immunol. Meth. 33, 33(1980).
Catelog Number :   
    51022
Product Name :   
     Flubida-2

Description:

Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidinchimera proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases. Flubida-2 is membrane-permeable and thus can be delivered into cells via simple incubation with the probe in a buffer.
  • Off-white solid soluble in DMSO
  • Store at 4°C
  • C43H48N4O9S
  • Mwt: 812.93

Chemical Structure of 51022

Reference:

Chemistry & Biology 7, 197(2000).
Catelog Number :   
    51023
Product Name :   
     5-carboxy-2',7'-dichlorosulfonefluorescein

Description:

The pKa of 5-carboxy-2',7'-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.
  • ex\em= 509/532nm (pH 9)
  • e = 92,000
  • Yellow solid soluble in DMSO, DMF or a buffer (pH >6)
  • Store at room temperature and protect from light
  • C20H10Cl2O8S
  • Mwt: 481.26

Chemical Structure of 51023

Reference:
Catelog Number :   
    51024
Product Name :   
     Flubi-2

Description:

Flubi-2 is the hydrolyzed product of the site-directed fluorogenic probe Flubida-2 (#51022) and can be used as a standard for calibration. The dye can also be microinjected into cells to probe the local pH where avidin-chimera proteins are located.
  • Yellow solid soluble in DMSO
  • Store at 4°C
  • C39H44N4O8S
  • Mwt: 728.85

Chemical Structure of 51024

Reference:

Chemistry & Biology 7, 197(2000)
Catelog Number :   
    90040
Product Name :   
     5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester

Description:

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The fluorescence of the dye is relatively insensitive to pH change and the labeling has been shown to be stable to formaldehyde or glutaldehyde fixation condition.
  • ex\em(of hydrolysis product at pH 7) = 504/529 nm
  • White solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C29H17Cl2NO11
  • Mwt: 626

Reference:

Development 116, 1087(1992).
Catelog Number :   
    90041
Product Name :   
     5(6)-CFDA, SE (5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester)

Description:

5(6)-CFDA, SE is a useful fluorescent tracer that can passivley diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases. The labeling has been shown to be stable to formaldehyde or glutaldehyde fixation condition.
  • ex\em(of hydrolysis product at pH 7) = 495/519 nm
  • White solid soluble in DMSO or DMF
  • Store at -20°C and protect from light
  • C29H19NO11
  • Mwt: 557 ! [150347-59-4]

Reference:

1) J. Cell Biol. 101, 610(1985); 2) J. Cell Biol. 103, 2649(1986); 3) Cell Transplantation 3, 397(1994).

 

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International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07