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Other Related Products for Calcium Studies
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Catelog Number :
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59000 |
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Product Name :
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Pluronic F-127 (free sample
per order of AM esters) |
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Description:
Nonionic detergent useful for solubilizing large dye
molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and
Rhod-2/AM to facilitate cell loading. Pluronic F-127 is
usually dissolved in DMSO or water by gentle heating.
Pluronic F-127 is a registered trade mark of BASF. |
Reference:
Poenie, M. et al. Science 233,
886(1986);
Drummond, I.A.S., et al. J. Biol. Chem. 262, 12801(1987).
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Catelog Number :
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59001 |
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Product Name :
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A-23187 Free Acid
(calcimycin; or Calcium Ionophore III) |
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Description:
A-23187 is a calcium ionophore that rapidly equilibrates
intracellular and extracellular calcium concentrations. It
is commonly used for in situ calibrations of
fluorescent calcium indicators. The ionophore allows Mn 2+
to enter the cells and quench intracellular fluorescence of
calcium indicators.
- White solid soluble in DMSO.
- Store at 4°C and protect from light.
- C29H37N3O6.
- Mwt: 523.6.
- [52665-69-7].
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Chemical Structure of 59001
Reference:
Suzuki, K. et al. Anal. Chem. 61, 382 (1989);
Caron-Leslie, L.A., et al. FASEB J. 8, 639 (1994);
Merck Index 12, 1678;
Saunders, M.J., et al. Science 217, 943 (19 |
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Catelog Number :
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59002 |
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Product Name :
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EDC (EDAC;
1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) |
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Description:
EDC (or EDAC) has recently been found to be very useful for
fixing in situ chelators, including the fluorescent
ion indicators in this catalogue. The fixation of ion
indicators makes it useful for post histological studies
following the physiological experiments. EDC is also a
widely used reagent to activate carboxy groups for amine
couplings.
- White solid soluble in water.
- Store desiccated at -20°C.
- C8H18ClN3.
- Mwt: 191.7.
- [25952-53-8].
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Reference:
Tymianski, M. et al. Cell Calcium 21(3),
175(1997);
Tetrahedron Lett. 34, 7685(1993). |
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Catelog Number :
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59003 |
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Product Name :
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TPEN (Full name:
tetrakis-(2-pyridylmethyl)ethylenediamine) |
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Description:
TPEN is an excellent heavy metal chelator while it does not
affect Ca ++, Mg ++ , Na + or
K + concentrations. Thus, TPEN is a useful tool to
identify effects of heavy metals (Zn ++, Fe ++/Fe +++,
Cu ++, and Mn ++, etc.) on fluorescent
indicators of Ca ++, Mg ++ ,Na +
and K +.
- Off-white solid soluble in water, DMSO or DMF
- Store at –20°C
- C26H28N6
- Mwt: 425
- [16858-02-9]
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Reference:
J. Biol. Chem. 260, 2719(1985) |
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Catelog Number :
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59004 |
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Product Name :
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Pluronic F-127 20% solution
in DMSO |
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Description:
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This is a ready-to-use format of the dispersing reagent and
is particularly useful for loading moisture-sensitive
materials into cells.
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Reference:
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Catelog Number :
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59005 |
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Product Name :
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Pluronic F-127, 10% in
sterile H2O |
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Description:
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This ready-to-use solution of the dispersing reagent is
useful for loading hydrophobic but moisture-sensitive
materials into cells.
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Reference: |
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Catelog Number :
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59006 |
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Product Name :
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4-Bromo A-23187, free acid |
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Description:
Similar to A-23187, 4-Bromo A-23187 is used for in situ
calibrations of fluorescent Ca 2+ indicators to
equilibrate intracellular and extracellular calcium
concentrations and to allow Mn 2+ to enter cells
to quench indicator fluorescence. However, since 4-bromo
A-23187 is nonfluorescent, it is preferred over the blue
fluorescent A-23187 for calibrations of UV-excited Ca 2+
indicators such as Indo-1 and Fura-2 in order to keep
background fluorescence to a minimum.
- White solid soluble in DMSO or EtOH
- Store at 4°C and protect from light
- C29H36BrN3O6
- Mwt: 602.52
- [76455-48-6]
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Reference:
1) Anal. Chem. 146, 349 (1985). |
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Catelog Number :
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59007 |
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Product Name :
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Ionomycin,calcium salt |
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Description:
Ionomycin is commonly used to modify intracellular calcium
concentrations and to calibrate fluorescent calcium
indicators. It is also known for its antipoliferative
effects.
- Yellowish oily solid soluble in DMSO or EtOH
- Store desiccated at -20°C
- C41H70O9Ca
- Mwt: 747.1
- [56092-82-1]
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Reference:
1) J. Biol. Chem. 275, 7071(2000); 2) J. Immunol.
155, 3297 (1995) J. Urol. 162, 916 (1999). |
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Catelog Number :
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59100 |
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Product Name :
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Calcium Calibration
Buffer Kit (zero and 10 mM CaEGTA, 2x50mL) |
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Description:
This kit provides you a range of calibration buffers with
accurate calcium concentrations and is useful for the
calibration of fluorescent Ca 2+ indicators. 1,2
This product contains components A and B.
component A(zero free Ca2+):Zero mM
CaEGTA (10 mM K2EGTA,100mM KCI and 10mM MOPS;pH
7.20)
component B(40uM free Ca2+):10 mM
CaEGTA (10 mM CaEGTA,100mM KCI and 10mM MOPS;pH 7.20)
For detailed information on application,please obtain our
protocol.
Colorless liquid
Store desiccated at 4°C.
Kit to be used within three months after receipt
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Reference:
1)Cell Calcyium 12,279(1991);2)Neuropharmacology
34,1423(1995) |
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Catelog Number :
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90082 |
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Product Name :
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DMSO, anhydrous
(Dimethyl sulfoxide, or methyl sulfoxide) |
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Description:
Anhydrous DMSO is recommended for preparing stock solutions
of AM ester dyes, reactive dyes and other related
moisture-sensitive products.
- Colorless liquid at room temperature.
- Store desiccated at room temperature.
- C2H6OS
- Mwt: 78.13
- [67-68-5]
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Reference: |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45