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Nerve Terminal Probes and Related Reagents Product Specification

Catelog Number :   
    00010
Product Name :   
     a-Bungarotoxin (from Bungarus multicinctus)

Description:

A potent inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). Also see our fluorescently labeled and biotin labeled a-bungarotoxins.
  • White solid soluble in water.
  • Store at -20°C and protect from light.
  • C338H529N97O105S11.
  • Mwt: 7984.
  • [11032-79-4].

Reference:

Zhang , et al. Neuron, 12, 167(1994).
Catelog Number :   
    00011
Product Name :   
     Fluorescein-a-bungarotoxin

Description:

Fluorescein-a-bungarotoxin binds to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. Also see our other dye- and biotin-labeled a- bungarotoxins.
  • ex\em = 494nm\518 nm.
  • Soluble in water.
  • Store at -20°C and protect from light, especially when in solution.
  • Mwt: ~ 8400.

Reference:

1) Science 196, 540(1977); 2) Muscle &Nerve 5, 140(1982)
Catelog Number :   
    00012
Product Name :   
     Tetramethylrhodamine-a-Bungarotoxin

Description:

Tetramethylrhodamine-a-Bungarotoxin binds to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. Also see other labeled a- bungarotoxin products.
  • ex\em= 553nm\577nm.
  • Soluble in water.
  • Store at -20°C and protect from light, especially when in solution.
  • Mwt: ~8400.

Reference:

Anderson, et al. J. Cell Biol. 99, 1769(1984)
Catelog Number :   
    00015
Product Name :   
     Sulforhodamine 101-a-bungarotoxin (also called Texas Red-a-bungarotoxin)

Description:

Sulforhodamine 101-a-bungarotoxin binds to the nicotinic acetylcholine receptor with high affinity. Since the dye sulforhodamine 101 has a longer absorption and emission wavelengths (lex\lem= 593/613 nm) than tetramethylrhodamine-a-bungarotoxin (#00012 ), it gives a better spectral separation when a green fluorescent probe such as SynaptoGreen C4 (FM1-43, #70020 ) is also used in the experiment. Also see other labeled a- Bungarotoxins.
  • Soluble in water
  • Store at -20°C and protect from light, especially in solution
  • Mwt: 8600

    • Reference:

    1) J Cell Biol 150, 1385 (2000); 2) Neuron 23, 675(1999).
    Catelog Number :   
    		    00017
    Product Name :   
    		     Biotin-XX-a-Bungarotoxin

    Description:

    Biotinylated a-bungarotoxin is useful in the affinity column isolation of the nicotinic AChR using an avidin or streptavidin agarose.1 Nicotinic AChRs labeled with biotin-XX-a-bungarotoxin can be localized using enzymeor fluorophore-labeled conjugates of avidin or streptavidin.2,3
    • White lyophilized solid soluble in water
    • Store at -20°C and protect from light
    • Mwt: ~8400

    Reference:

    1) J Cell Biol 125, 661-668 (1994); 2) J Biol Chem 268, 25108(1993); 3) Proc Natl Acad Sci U S A 77, 4823(1980)
    Catelog Number :   
    		    00060
    Product Name :   
    		     Tetrodotoxin, citrate free

    Description:

    Tetrodotoxin (TTX) reversibly blocks the excitable sodium channels and has been a widely used tool for the studies of excitable membranes of nerve, skeletal and cardiac cells. Biotium offers a very high quality TTX with a very competitive price. Please also see our TTX with citrate (00061).
    • White solid soluble in slightly acidic ( pH 4.8 ) buffer.
    • Store at -20°C.
    • C11H17N3O8.
    • Mwt: 319.3.
    • [4368-28-9].

    Chemical Structure of 00060

    Reference:

    1) Lysko, P.G. et al. Stroke 25, 2476(1994); 2) Hu, S.L. and Kao, C.Y. Toxicon 23, 723(1985); 3) Nakamura, M. and Yasumoto, T. Toxicon 23, 271(1985).
    Catelog Number :   
    		    00061
    Product Name :   
    		     Tetrodotoxin, with citrate (purity: >98%)

    Description:

    Tetrodotoxin (TTX) reversibly blocks the excitable sodium channels and has been a widely used tool for the studies of excitable membranes of nerve, skeletal and cardiac cells. Biotium offers a very high quality TTX with a very competitive price. TTX with citrate is a lyophilized mixture of 1 mg high purity TTX and 5 mg pH 4.8 citrate buffer.Please also see TTX, citrate-free (#00060).
    • White solid soluble in slightly acidic (pH 4.8) buffer
    • Store at 4°C
    • C11H17N3O8
    • Mwt: 319.3
    • [4368-28-9]

    Reference:

    1) Lysko, P.G. et al. Stroke 25, 2476(1994); 2) Hu, S.L. and Kao, C.Y. Toxicon 23, 723(1985); 3) Nakamura, M. and Yasumoto, T. Toxicon 23, 271(1985).
    Catelog Number :   
    		    70019
    Product Name :   
    		     SynaptoRedTMC2M (special packaging)

    Description:

    SynaptoRed C2M (also known as FM5-95, a trademark of Molecular Probes, Inc.) is a red fluorescent probe used to follow synaptic vesicles. SynaptoRed C2M is slightly more water soluble than SynaptoRed C2 (also known as FM4-64).

    Reference:

    1) Science 297, 1525-1531 (2002);

  • 2) Cell 107, 605-616 (2001)

     

     

    		•
    Catelog Number :   
    		    70020
    Product Name :   
    		     SynaptoGreenTM C4 (also known as FM1-43, a trademark of Molecular Probes, Inc.)

    Description:

    A widely used green fluorescent dye for following synaptic activities by staining synaptic vesicles at the synapse or neuromuscular junctions.1 When used in combination with the red fluorescent dye SynaptoRed 70021 ), synapses or neuromuscular junctions can be imaged independently in two colors. Recent research results from Tsien's2 and Kay's3 labs showed that brain slices can be stained with the styryl dye SynaptoGreen when SR101 (Sulforhodamine 101, 80101 ) or ADVASEP-7(70029 ) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. 

     

    • ex\em: 510/625 nm(in MeOH); 480/598 nm(in membranes). Red solid soluble in water.
    • C30H49Br2N3. Mwt: 612. 

    Please also see AM1-43(70024). 

    Chemical Structure of 70020

    Reference:

    1) J. Neurosci. 12, 363(1992); 
    2) Science. 255, 200(1992); 
    2) Neuron 24, 803(1999); 
    3) Neuron 24, 809(1999).
    Catelog Number :   
    		    70021
    Product Name :   
    		     SynaptoRedTM C2 (also known as FM4-64, a trademark of Molecular Probes, Inc.)

    Description:

    A widely used red fluorescent dye for following synaptic activities by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen (70020 ), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed has also been used to study endocytosis in Dictyostelia. Dark purple solid soluble in water.

     

    C30H45Br2N3. Mwt: 608. 

    Please also see AM1-43(70024 ). 

    Chemical Structure of 70021

    Reference:

    1) J. Neurosci. 12, 363(1992); 
    2) Science. 255, 200(1992); 
    3) J. Cell Biol. 121, 1311(1993).
    Catelog Number :   
    		    70022
    Product Name :   
    		     SynaptoGreenTM C4 (special packaging)

    Description:

    A widely used green fluorescent dye for following synaptic activities by staining synaptic vesicles at the synapse or neuromuscular junctions.1 When used in combination with the red fluorescent dye SynaptoRed(70021 ), synapses or neuromuscular junctions can be imaged independently in two colors. Recent research results from Tsien's2 and Kay's3 labs showed that brain slices can be stained with the styryl dye SynaptoGreen when SR101 (Sulforhodamine 101, 80101 ) or ADVASEP-7(70029 ) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices.
    • ex\em: 510/625 nm(in MeOH); 480/598 nm(in membranes). Red solid soluble in water. 
    • C30H49Br2N3. Mwt: 612. 

    Please also see AM1-43 (70024 ). 

    Reference:

    1) J. Neurosci. 12, 363(1992); 
    2) Science. 255, 200(1992); 
    2) Neuron 24, 803(1999); 
    3) Neuron 24, 809(1999).
    Catelog Number :   
    		    70023
    Product Name :   
    		     SynaptoGreenTM C3

    Description:

    SynaptoGreenTM C3  is similar to SynaptoGreen (70020 ) except that the lipophilic tail is one carbon shorter and also the hydrophilic end is a trimethylammonium group. Thus SynaptoGreen II is slightly more water soluble than SynaptoGreen.

     

    • ex\em: 510/625 nm(in MeOH); 480/598 nm(in membranes).
    • Red solid soluble in water.
    • C25H39Br2N3.
    • Mwt: 542.

    Please also see AM1-43(70024 ). 

    Chemical Structure of 70023

    Reference:

    1) J. Neurosci. 12, 363(1992); 
    2) Science. 255, 200(1992); 
    2) Neuron 24, 803(1999); 
    3) Neuron 24, 809(1999).
    Catelog Number :   
    		    70024
    Product Name :   
    		     AM1-43

    Description:

    In collaboration with Dr. Guosong Liu's lab at MIT, Biotium has developed AM1-43 as a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly as our SynaptoGreen (70020 ) (equivalent to FM1-43, a trademark of Molecular Probes, Inc.) does, but with the additional property of being fixable. Thus, AM1-43 is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired. AM1-43 and FM1-43 have identical excitation and emission wavelengths.

    Chemical Structure of 70024

    Reference:
    Catelog Number :   
    		    70026
    Product Name :   
    		     SynaptoGreenTM C3 (special packaging)

    Description:

    SynaptoGreenTM C3  is similar to SynaptoGreen (70020) except that the lipophilic tail is one carbon shorter and also the hydrophilic end is a trimethylammonium group. Thus SynaptoGreen II is slightly more water soluble than SynaptoGreen.

     

    • ex\em: 510/625 nm(in MeOH); 480/598 nm(in membranes).
    • Red solid soluble in water.
    • C25H39Br2N3.
    • Mwt: 542.

    Please also see AM1-43 (70024)

    Reference:

    1) J. Neurosci. 12, 363(1992); 
    2) Science. 255, 200(1992); 
    2) Neuron 24, 803(1999); 
    3) Neuron 24, 809(1999).
    Catelog Number :   
    		    70027
    Product Name :   
    		     SynaptoRedTM C2 (special packaging)

    Description:

    A widely used red fluorescent dye for following synaptic activities by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed has also been used to study endocytosis in Dictyostelia. Dark purple solid soluble in water.

     

    C30H45Br2N3. Mwt: 608. 

    Please also see AM1-43 (70024)

    Reference:

    1) J. Neurosci. 12, 363(1992); 
    2) Science. 255, 200(1992); 
    3) J. Cell Biol. 121, 1311(1993).
    Catelog Number :   
    		    70028
    Product Name :   
    		     SynaptoRedTMC2M (also known as FM5-95, a trademark of Molecular Probes, Inc.)

    Description:

    SynaptoRed C2M (also known as FM5-95, a trademark of Molecular Probes, Inc.) is a red fluorescent probe used to follow synaptic vesicles. SynaptoRed C2M is slightly more water soluble than SynaptoRed C2 (also known as FM4-64).

    Reference:

    1) Science 297, 1525-1531 (2002);

  • 2) Cell 107, 605-616 (2001)
    		•
    Catelog Number :   
    		    70029
    Product Name :   
    		     ADVASEP-7

    Description:

    ADVASEP-7 is a sulfonated b-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (also called FM1-43 (#70020)) to stain brain slices.
    • White solid soluble in water
    • Store at 4°C

    Reference:

    Kay, A. R., A. Alfonso, et al. "Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, Lamprey and Rat." Neuron 24: 809-817 (1999)
    Catelog Number :   
    		    70030
    Product Name :   
    		     Nerve Terminal Staining Kit I

    Description:

    This kit contains 5x1 mg SynaptoGreen C4 (70020, equivalent to FM1-43, a trademark of Molecular Probes, Inc.) and 250 mg ADVASEP-7 (sulfobutyl b-cyclodextrin) ((70029)).

    Reference:
    Catelog Number :   
    		    70031-1
    Product Name :   
    		     Nerve Terminal Staining Kit II

    Description:

    This kit contains 1mg AM1-43 (70024) and 100 mg SCAS ((70037)).

    Reference:
    Catelog Number :   
    		    70032
    Product Name :   
    		     Nerve Terminal Staining Kit III

    Description:

    This kit contains 5x1 mg SynaptoGreen C4 ((70022), equivalent to FM1-43, a trademark of Molecular Probes, Inc.) and 100 mg Sulforhodamine 101 ((80101)).

    Reference:
    Catelog Number :   
    		    70034
    Product Name :   
    		     Nerve Terminal Staining Kit V

    Description:

    This kit contains 5x1 mg SynaptoRed C2((70027), equivalent to FM4-64, a trademark of Molecular probes, Inc.) and 250 mg ADVASEP-7(70029).

    Reference:
    Catelog Number :   
    		    70035
    Product Name :   
    		     Nerve Terminal Staining Kit VI

    Description:

    This kit contains 5x1 mg SynaptoRed C2((70027), equivalent to FM4-64, a trademark of Molecular probes, Inc.) and100 mg Sulforhodamine 101 (80101).

    Reference:
    Catelog Number :   
    		    70036
    Product Name :   
    		     AM2-10

    Description:

    Bioutium synthesized AM2-10 as a fixable analog of SynaptoGreen C2 (FM2-10). The utility of this probe is still under evaluation. See Table 1 for more data. Dark red solid soluble in water.C25H37Cl3N4 Mwt: 503.5

    Chemical Structure of 70036

    Reference:
    Catelog Number :   
    		    70037
    Product Name :   
    		     SCAS(4-sulfonato calix[8]arene, sodium salt)

    Description:

    SCAS is a quencher developed by Bioutium to reduce background fluorescence when using our fixable AM dyes, SynaptoGreen and SynaptoRed dyes.Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated washings.
    • Off-white soild soluble in water
    • C56H40Na8O32S8
    • Mwt 1665.34.

    Chemical Structure of 70037

    Reference:
    Catelog Number :   
    		    70038
    Product Name :   
    		     AM1-44

    Description:

    AM1-44 is improved upon AM1-43 with better fixability. The dye can also be used as a general probe for following endocytosis.

    Reference:
    Catelog Number :   
    		    70039
    Product Name :   
    		     AM4-65

    Description:

    AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed C2 (also called FM4-64), but it has an additional amine group at the hydrophlic end of the molecule, making the dye fixable with formaldehyde or glutaldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.

    Reference:
    Catelog Number :   
    		    70040
    Product Name :   
    		     SynaptoRedTM C1

    Description:

    SynaptoRed C1 is similar to the widely used red fluorescent nerve terminal dye SynaptoRed C2(also called FM4-641) except that the lipophilic tail of the latter is one carbon longer than that of the former. Thus, SynaptoRed C1 is expected to have a greater "off-rate" than SynaptoRed C2 does.

     

    • Dark purple solid soluble in water
    • C28H41Br2N3
    • Mwt: 580

    Chemical Structure of 70040

    Reference:

     

     
    Catelog Number :   
    		    70041
    Product Name :   
    		     SynaptoRedTM C1(special packaging)

    Description:

    SynaptoRed C1 is similar to the widely used red fluorescent nerve terminal dye SynaptoRed C2(also called FM4-641) except that the lipophilic tail of the latter is one carbon longer than that of the former. Thus, SynaptoRed C1 is expected to have a greater "off-rate" than SynaptoRed C2 does.

     

    • Dark purple solid soluble in water
    • C28H41Br2N3
    • Mwt: 580

    Reference:
    Catelog Number :   
    		    70042
    Product Name :   
    		     SynaptoGreenTM C1

    Description:

    SynaptoGreen C1 has the shortest (one-carbon) lipophilic tail and is the most water soluble of the nerve terminal dye series. Thus it is expected to have the slowest" on-rate" but the fastest "off-rate".

     

    • Red solid soluble in water.
    • C24H37Br2N3.
    • Mwt: 528.

    Chemical Structure of 70042

    Reference:

     

     
    Catelog Number :   
    		    70043
    Product Name :   
    		     SynaptoGreenTM C1(special packaging)

    Description:

    SynaptoGreen C1 has the shortest (one-carbon) lipophilic tail and is the most water soluble of the nerve terminal dye series. Thus it is expected to have the slowest" on-rate" but the fastest "off-rate".

     

    • Red solid soluble in water.
    • C24H37Br2N3.
    • Mwt: 528.

    Reference:
    Catelog Number :   
    		    70044
    Product Name :   
    		     SynaptoGreenTM C2

    Description:

    SynaptoGreen C2 is more water soluble than SynaptoGreen C4 and thus has a faster de-staining rate than the latter.

     

    • Red solid soluble in water
    • C26H41Br2N3
    • Mwt: 556

    Chemical Structure of 70044

    Reference:

    Proc. R. Soc. Lond. B 255, 61(1994)
    Catelog Number :   
    		    70045
    Product Name :   
    		     SynaptoGreenTM C2(special packaging)

    Description:

    SynaptoGreen C2 is more water soluble than SynaptoGreen C4 and thus has a faster de-staining rate than the latter.

     

    • Red solid soluble in water
    • C26H41Br2N3
    • Mwt: 556

    Reference:

    Proc. R. Soc. Lond. B 255, 61(1994).
    Catelog Number :   
    		    70046
    Product Name :   
    		     SynaptoGreenTM C5

    Description:

    SynaptoGreen C5 is similar to SynaptoGreen C4 70020 except that the lipophilic tail is one carbon longer. Thus SynaptoGreen C5 is less water soluble than SynaptoGreen C4 and thus faster staining rate but slower de-staining rate.

     

  • ex\em: 510/625 nm(in MeOH).
  • Red solid soluble in water.
  • C32H53Br2N3.
  • Mwt: 640.
    		•

    Chemical Structure of 70046

    Reference:

     

     
    Catelog Number :   
    		    70047
    Product Name :   
    		     SynaptoGreenTM C5(special packaging)

    Description:

    SynaptoGreen C5 is similar to SynaptoGreen C4 70020 except that the lipophilic tail is one carbon longer. Thus SynaptoGreen C5 is less water soluble than SynaptoGreen C4 and thus faster staining rate but slower de-staining rate.

     

  • ex\em: 510/625 nm(in MeOH).
  • Red solid soluble in water.
  • C32H53Br2N3.
  • Mwt: 640.
    		•

    Reference:

     

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    International 

    +32 (0) 16 58 90 45

    +32 (0) 16 50 90 45

    France

    01 43 25 01 50

    01 43 25 01 60

    Italy

    02 36 00 65 93

    02 36 00 65 94

    Germany

    0241 6085 13140

    0241 6085 33033
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    Last modified: feb-07