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Membrane Potential Dyes
Product Specification
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Catelog Number :
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61010 |
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Product Name :
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Di-4-ANEPPS |
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Description:
Di-4-ANEPPS is a fast-responding membrane potential dye. The
changes in the fluorescence excitation intensities of the
dye are correlated to the changes in the membrane potential
of the cell.
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 ex\em(MeOH)
= 496/ 705nm
- Orange red solid soluble in DMF, ethanol or DMSO
- Store at 4°C and protect from light, especially in
solution
- C28H36N2O3S.
- Mwt: 481
- [90134-00-2]
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Chemical Structure of 61010
Reference:
1) Davidenko, J.M., et al. Nature 355, 349(1992); 2)
Fromherz, P., et al. Biochim. Biophys. Acta 1068, 149(1991). |
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Catelog Number :
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61011 |
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Product Name :
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DiBAC4(3)
(bis-(1,3-dibarbituric acid)-trimethine oxanol) |
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Description:
DiBAC 4(3) is a so-called translational membrane
potential dye that redistributes within the cell membrane
when membrane potential changes. 1,2 The
fluorescence of the dye is enhanced when the dye enters the
cell membrane as a result of membrane depolarization. The
rate of fluorescence response of the dye to membrane
potential change is usually slower than that of the styryl
dye di-4-ANEPPS, but the fluorescence signal change for the
former is significantly larger than for the latter dye. A
new and potentially very important application for DiBAC 4(3)
is its use in high throughput drug screening 3
- Orange solid soluble in DMSO or DMF.
- Store at -20°C and protect from light.
- C27H40N4O6.
- Mwt: 519.
- [70363-83-6]
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Chemical Structure of 61011
Reference:
1) Chem. Phys. Lipids 69, 137(1994); 2)
Biochim. Biophys. Acta 771, 208(1984); 3) J. Biomol.
Screening 1(2), 75(1996). |
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Catelog Number :
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61012 |
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Product Name :
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Di-8-ANEPPS |
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Description:
ANEPPS dyes are fast responding membrane potential dyes. 1
Biotium offers both Di-4-ANEPPS and Di-8-ANEPPS.
Di-4-ANEPPShas the problem of being internalized in cells
over long-term, so it is mainly used for short-term
experiments. However, because Di-8-ANEPPS is more
lipophilic, the dye is better retained in the outer leaflet
of cell plasma membranes and thus more suitable for
long-term membrane potential studies. Di-8-ANEPPS is also
more photostable and less phototoxic than Di-4-ANEPPS. 2,3
 ex\ em(MeOH)
= 498 nm / 713 nm.
- Orange red solid soluble in DMF, ethanol or DMSO.
- Store at 4°C and Protect from light, especially when
in solution.
- C36H52N2O3S.
- Mwt:593
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Chemical Structure of 61012
Reference:
1) J. Neurosci. 15, 1392(1995); 2)Neuron 13,1187(1994);3)Neuron,9,393(1992). |
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Catelog Number :
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61013 |
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Product Name :
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Di-2-ANEPEQ (JPW
1114) |
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Description:
Di-2-ANEPEQ, also called JPW1114, is a fast-responding
membrane potential dye. It is highly water-soluble and is
usually introduced into cells by microinjection. The dye can
also be applied to brain tissues topically.
- Orange red solid soluble in H2O, ethanol
or DMSO
- Store at -20°C and protect from light, especially in
solution
- C26H35Br2N3
- Mwt: 547.12
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Chemical Structure of 61013
Reference:
1) Biol. Bull. 198, 1(2000); 2) Proc. Natl. Acad Sci.
USA 94, 7621(1997). |
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Catelog Number :
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61014 |
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Product Name :
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Di-8-ANEPPQ |
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Description:
Di-8-ANEPPQ is a fast-responding membrane potential dye. It
is more hydrophobic than Di-2-ANEPEQ and is useful for
potential-sensitive retrograde labeling of neurons.
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C38H59Br2N3
- Mwt: 717.70
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Chemical Structure of 61014
Reference:
1) J. Neurosci. Methods. 70, 121(1996); 2) J.
Neurosci. Methods 70, 111(1996). |
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Catelog Number :
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61015 |
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Product Name :
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Di-12-ANEPPQ |
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Description:
Di-12-ANEPPQ is a fast-responding membrane potential dye. It
is more hydrophobic than Di-8-ANEPPQ and is useful for
potential-sensitive retrograde labeling of neurons.
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C46H75Br2N3
- Mwt: 827.4
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Chemical Structure of 61015
Reference:
1) J. Neurosci. Methods. 70, 121(1996); 2) J.
Neurosci. Methods 70, 111(1996). |
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Catelog Number :
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61016 |
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Product Name :
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RH414 |
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Description:
The styryl dye RH414 is a fast-responding potentiometric
probe that is primarily used for functional imaging of
neurons. 1
 ex\ em
(MeOH) = 532/ 716nm2
- Orange red soluble in H2O or DMSO
- Store at -20°C and protect from light
- C28H43Br2N3
- Mwt: 581.48
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Chemical Structure of 61016
Reference:
1) Neuroscience 31, 613 (1989); 2) The data is for
methanol solution of the dye. In cell membranes, spectra of styryl dyes
are typically blue-shifted by as much as 20 nm for absorption or
excitation and 80 nm for emission. |
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Catelog Number :
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61017 |
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Product Name :
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RH421 |
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Description:
RH421 is a fast responding membrane potential dye,
exhibiting >20% fluorescence change per 100 mV on
neuroblastoma cells.
 ex\ em
= 515/704 nm (MeOH).
- Red solid soluble in DMSO, EtOH or DMF.
- Store at -20°C and protect from light, especially in
solution.
- C29H42N2O3S.
- Mwt: 498.72.
- [107610-19-5].
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Chemical Structure of 61017
Reference:
Biophys. J. 42, 195(1983). |
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Catelog Number :
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61018 |
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Product Name :
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RH237 |
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Description:
The styryl dye RH237 is a fast-responding potentiometric
probe that is primarily used for functional imaging of
neurons. 1
 ex\ em(MeOH)
= 528/ 782nm2
- Dark solid soluble in DMSO
- Store at -20°C and protect from light
- C29H40N2O3S
- Mwt: 496.71
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Chemical Structure of 61018
Reference:
1) Neuroscience 31, 613 (1989); 2) The data is for
methanol solution of the dye. In cell membranes, spectra of styryl dyes
are typically blue-shifted by as much as 20 nm for absorption or
excitation and 80 nm for emission. |
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Catelog Number :
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61019 |
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Product Name :
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RH795 |
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Description:
The styryl dye RH795 is a fast-responding potentiometric
probe that is primarily used for functional imaging of
neurons. The dye is spectrally similar to RH414 (# 61016),
but exhibits different physiological effects during
staining. For example, RH414 causes arterial constriction
during cortex staining, while RH795 does not. 1,2
 ex\ em(MeOH)
= 530/ 712nm3
- Orange red solid soluble in H2O
- Store at -20°C and protect from light
- C26H39Br2N3O2
- Mwt: 585.42
- [172807-13-5]
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Chemical Structure of 61019
Reference:
1) Nature J. 324, 361(1986); 2) J. Neurosci. 14,
2545(1994); 3) The data is for methanol solution of the dye. In cell
membranes, spectra of styryl dyes are typically blue-shifted by as much
as 20 nm for absorption or excitation and 80 nm for emission.
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Catelog Number :
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70007 |
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Product Name :
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DiOC5(3)
(3,3'-Dipentyloxacarbocyanine, iodide) |
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Description:
DiOC5(3) and DiOC6(3) are two of the most widely used
carbocyanine dyes used for membrane potential measurements
 ex\ em(MeOH)
= 482/ 497nm
- Orange solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C27H33IN2O2
- Mwt: 544.47
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Chemical Structure of 70007
Reference:
J. Biol. Chem. 270, 3788 (1995). |
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Catelog Number :
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70008 |
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Product Name :
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DiOC2(3)
(3,3'-Diethyloxacarbocyanine, iodide) |
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Description:
DiOC 2(3) has been used for measuring membrane
potentials in bacteria. The green fluorescent dye forms red
fluorescent aggregates with increasing membrane potential.
The spectral shifting phenomenon has been exploited to
measure membrane potential ratiometrically.
 ex\ em(MeOH)
= 482/ 497nm
- Orange solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C21H21IN2O2
- Mwt: 460.31
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Chemical Structure of 70008
Reference:
1) Biophysics J. 56, 979(1989). |
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Catelog Number :
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70009 |
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Product Name :
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DiOC6(3)
(3,3'-dihexyloxacarbocyanine,iodide) |
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Description:
DiOC 6(3) is a green fluorescent membrane dye that
has been used to stain the ER in both live and fixed cells. 1,2
The dye has also been used to study structural interactions
and dynamics of the ER in neurons 3,4 and yeast. 5
In addition, the dye is widely used as a memberane potential
dye. 6
 ex\ em(MeOH)
= 484/501nm
- Orange solid soluble in DMSO or DMF
- Store at 4°C and protect from light, especially in
solution
- 29H37IN2O2.</
- Mwt: 572.53
- [53213-82-4]
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Chemical Structure of 70009
Reference:
Ref: 1) J. Cell Biol. 103, 1557(1986); 2) Cell 38,
101(1984); 3) J. Cell Biol. 127, 1021(1994);, 6050(1980); 4) Nature,
310, 53(1984); 5) Cell Motil. Cytoskeleton, 25, 111(1993); |
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Catelog Number :
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70015 |
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Product Name :
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DiIC1(5)
(1,1'-dimethyl-3,3,3',3'-tetramethylindodicarbocyanine, iodide) |
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Description:
The carbocyanine dye DiIC 1(5) has been used to
measure mitochondrial membrane potentials in apoptotic
cells. Loss of membrane potential in apoptotic cells is
reflected by a decrease of fluorescence signal in the
infrared channel.
 ex\ em
(MeOH) = 638/ 658nm
- Dark green solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C27H31IN2
- Mwt: 510.46
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Chemical Structure of 70015
Reference:
1) Cytometry 33, 333(1998). |
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Catelog Number :
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70016 |
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Product Name :
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Tetramethylrhodamine ethyl
ester, perchlorate (TMRE) |
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Description:
TMRM ( 70017 ) and TMRE (
70016 ) are said to be the preferred
dyes for quantitative measurements of membrane potentials
using Nernst equation. The dyes do not form aggregates in
cell membarnes and interact with membrane proteins
minimally. Thus, the transmembrane distribution of the dyes
is directly related to the membrane potential via Nernst
equation.
 ex\ em(MeOH)
= 549/574nm
- Red solid soluble in DMSO, DMF or EtOH
- Store at 4°C and protect from light
- C26H27ClIN2O7.
- Mwt: 515
- [115532-52-0]
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Chemical Structure of 70016
Reference:
1) Meth. Cell Biol. 38, 195(1993); 2) Meth. Cell
Biol. 30, 193(1989); 3) Biophys. J. 56, 1053(1989); 4) Biophys., J.
53, 785(1988). |
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Catelog Number :
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70017 |
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Product Name :
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Tetramethylrhodamine methyl
ester, perchlorate(TMRM) |
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Description:
TMRM ( 70017 ) and TMRE (
70016 ) are said to be the preferred
dyes for quantitative measurements of membrane potentials
using Nernst equation. The dyes do not form aggregates in
cell membarnes and interact with membrane proteins
minimally. Thus, the transmembrane distribution of the dyes
is directly related to the membrane potential via Nernst
equation.
 ex\ em(MeOH)
= 548/573nm
- Red solid soluble in DMSO, DMF or EtOH
- Store at 4°C and protect from light
- C25H25ClIN2O7.
- Mwt: 501
- [115532-50-8]
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Chemical Structure of 70017
Reference:
1) Meth. Cell Biol. 38, 195(1993); 2) Meth. Cell
Biol. 30, 193(1989); 3) Biophys. J. 56, 1053(1989); 4) Biophys., J. 53,
785(1988).
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45