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Maleimides and Other Thiol-Reactive Reagents
Product Specification
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Catelog Number :
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91029 |
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Product Name :
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5(6)-Carboxyrhodamine 110-C5-maleimide (5(6)-CR110-C5-Maleimide) |
Description:
CR110-C5-maleimide is a maleimide derivative of the green
fluorescent dye carboxyrhodamine 110. The dye has spectral
properties ( lex\ lem
(MeOH) = 502/524 nm) similar to those of fluorescein
(fluorescein-5-maleimide, for example), but the
photostability of the dye is among the best of all green
fluorescent dyes. Thus, the dye is a superior green
fluorescent dye for labeling thiols.
Orange red solid soluble in DMF or DMSO.
Store at -20°C and protect from light, especially in
solution.
C31H30N4O6.
Mwt: 554.59. |
Chemical Structure of 91029
Reference: |
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Catelog Number :
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91040 |
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Product Name :
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5(6)-Carboxytetramethylrhodamine-C5-maleimide
(5(6)-TAMRA-C5-maleimide) |
Description:
5(6)-Carboxytetramethylrhodamine-C5-maleimide is a superior
alternative to tetramethylrhodamine maleimide. The C5 linker
molecule enhances the fluorescence after the dye is
conjugated to proteins.
- lex\lem
(MeOH) = 540/565 nm).
- Red solid soluble in DMF or DMSO.
- Store at -20°C and protect from light, especially in
solution.
- C34H34N4O6.
- Mwt: 594.66.
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Chemical Structure of 91040
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Catelog Number :
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91041 |
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Product Name :
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5(6)-Carboxy-X-rhodamine-C5-maleimide (5(6)-ROX-C5-maleimide) |
Description:
5(6)-Carboxy-X-rhodamine-C5-maleimide is a long wavelength
thiol-reactive rhodamine dye . The C5 linker molecule
enhances the fluorescence after the dye is conjugated to
proteins.
- lex\lem
(MeOH) = 568/595 nm.
- Red solid soluble in DMF or DMSO. Store at 20°C and
protect from light, especially in solution.
- C42H42N4O6.
- Mwt: 698.81.
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Chemical Structure of 91041
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Catelog Number :
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91046 |
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Product Name :
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ABD-F |
Description:
ABD-F is a useful reagent for the sensitive and specific
detection of thiols both by TLC and HPLC. 1,2 When
combined with a reducing agent such as tributylphosphine, it
can also be used for the determination of disulfides in
peptides and proteins. 3,4
- lex\lem(N-acetylcysteine
conjugate at pH 2) = 375/508 nm.
- Of-white solid soluble in DMSO or DMF.
- C7H7FN3O3S.
- Mwt. = 232.
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Chemical Structure of 91046
Reference:
1) Anal. Chem. 56, 2461(1984); 2) J.
Pharm. Biomed. Anal 24, 1137(2001); 3) Anal. Biochem.
214, 128(1993); 4) J. Biol. Chem. 264, 7185(1989). |
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Catelog Number :
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90059 |
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Product Name :
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Biotin
ethylenediamine iodoacetamide
(N-(biotinoyl)-N'-(iodoacetyl)ethylenediamine) |
Description:
Biotin ethylenediamine iodoacetamide is readily coupled to
thiols.
- Off-white solid soluble in DMF.
- Store at -20°C
- C14H23IN4O3S.
- Mwt = 454.33.
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Chemical Structure of 90059
Reference:
1) J Biol Chem 274, 4722 (1999); 2) Biochem. J. 251,
935(1988). |
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Catelog Number :
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90058 |
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Product Name :
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Biotin-X-C5-maleimide
(N-(5-(6-((biotinoyl)amino)hexanoyl)amino)pentylmaleimide) |
Description:
Biotin-X-C 5-maleimide is readily and selectively
coupled to thiols.
- White solid soluble in DMSO, or DMF.
- Store at -20°C
- C27H39N5O7S.
- Mwt. = 521.67.
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Chemical Structure of 90058
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Catelog Number :
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91010 |
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Product Name :
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CPM |
Description:
CPM (full name:
7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin) is
probably the most widely used blue fluorescent
thiol-reactive dye. This maleimide derivative of coumarin is
essentially nonfluorescent until it reacts with thiols,
making it possible to quantify thiols without a separation
step. CPM is a good energy acceptor from tryptophan and a
good energy donor to fluorescein. Please also see our
MTS products. |
Chemical Structure of 91010
Reference:
1) Biochemistry, 22, 5369(1983);
2) Anal. Lett. 18, 393(1985);
3) J. Biol. Chem. 265, 14796(1990). |
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Catelog Number :
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91050 |
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Product Name :
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DTT |
Description:
Reference: |
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Catelog Number :
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92013 |
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Product Name :
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Eosin-5-maleimide |
Description:
Eosin-5-maleimide is a good photosensitizer and can be used
to selectively label thiols. It has a quantum yield of 0.57
for singlet oxygen generation, which makes it useful for
photoconversion of electron-rich materials such as
diaminobenzidine (DAB) or fluorescent dyes into highly
electron-dense materials for high resolution electron
microscopy studies.1,2 Additionally, eosin is a good
fluorescence acceptor for fluorescein (FAM), dansyl and
coumarin dyes in fluorescence resonance energy transfer
(FRET).
- lex\lem(MeOH)
= 524/545 nm
- e = 100,000
- Orange solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C24H9Br4NO7
- Mwt: 742.95
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Reference:
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Catelog Number :
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91028 |
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Product Name :
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Fluorescein-5-maleimide |
Description:
Fluorescein-5-maleimide is the most widely used green
fluorescent thiol-reactive dye. The reaction requires very
mild condition and is highly specific.
lex\lem
= 492/515 nm (pH 9) (Extinction coefficient = 83,000).
Yellow solid soluble in DMF. Store at -20°C and protect
from light.
Mwt: 427. |
Chemical Structure of 91028
Reference:
1) Anal. Lett. 18, 393(1985);
2) Biochemistry, 30, 2113(1991). |
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Catelog Number :
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92015 |
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Product Name :
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N-(1,10-Phenanthrolin-5-yl)iodoacetamide |
Description:
N-(1,10-Phenanthrolin-5-yl)iodoacetamide has been used to
label thiols to confer the metal-binding properties of the
phenanthroline chelator. For example, complexing of the
chelator with copper can be used to selectively cleave RNA
or DNA in the presence of hydrogen peroxide.
- Off-white solid soluble in DMF
- Store at -20°C and protect from light
- C14H10IN3O
- Mwt: 363.15
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Reference:
1) Nucleic Acids Res.. 22, 4789(1994); 2)
Bioconjugate Chem. 4, 69(1993); 3) Meth. Enzymol. 208, 414(1991); 4)
Anal. Rev. Biochem. 59, 207(1990); 5) J. Am. Chem. Soc. 111, 4941(1989);
6) Proc. Natl. Acad. Sci. USA 86, 9702(1989). |
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Catelog Number :
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91047 |
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Product Name :
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N-(5-Aminopentyl)maleimide, trifluoroacetic acid salt |
Description:
N-(5-Aminopentyl)maleimide is a cross-linking reagent that
can react with an amine-reactive group and a thiol group.
- White solid soluble in DMSO, DMF, or H2O.
- Store at -20°C and protect from light
- C11H15F3N2O4.
- Mwt. = 296.
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Chemical Structure of 91047
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Catelog Number :
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92014 |
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Product Name :
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Sulforhodamine 101
C5-maleimide (Texas RedTM C5-maleimide) |
Description:
Sulforhodamine 101 C5-maleimide is a long wavelength
fluorescent thiol-reactive dye.
- lex\lem(pH
9) = 582/600 nm
- e= 110,000
- Dark red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C40H42N4O8S2
- Mwt: 770.91
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Reference: |
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Catelog Number :
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91049 |
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Product Name :
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TCEP |
Description:
Reference: |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45