Product Specification

Catelog Number :   
    60010
Product Name :   
     DiI (DiIC18(3), or 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate)

Description:

DiI is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C18 carbon) hydrocarbon chains into the lipid bilayers. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons. The intense fluorescence and high photostability of the dye make it possible to visualize the fine structures (axons and dendrites) of the neurons. Due to its low toxicity and the tendency to give highly stable cell labeling, the dye has also been generally used for long term cell tracing of cells both in cultures and in living embryos or animals. Please see our ready-to-use CellBriteTM Cell Membrane Labeling Kits for labeling cell suspension in Bioscience Kits section.
  • ex\em(MeOH) = 549/565 nm
  • e = 148,000
  • Dark red solid soluble in ethanol, DMF or DMSO
  • Store at -20°C
  • C59H97ClN2O4.
  • Mwt: 933.88
  • [41085-99-8]

Chemical Structure of 60010

Reference:

1)Derzko, Z., et al. Biochemistry 19, 6050(1980); 2)Leuther, M.D., et al. J. Immunology 127, 893(1981); 3)Honig, M.G. and Hume, R.I. Trends in Neurosci. 9, 333(1989); 4)Honig, M.G. and Hume, R.I. J. Cell Biol. 103, 171(1986); 5)McConnell, S.K., et al. Science 245, 978(1989).
Catelog Number :   
    60011
Product Name :   
     DiO (3,3'-dioctadecyloxacarbocyanine, perchlorate)

Description:

DiO is another carbocyanine membrane dye that has applications similar to those of DiI (See #60010). However, DiO has green fluorescent emission and the lateral diffusion rate on the membranes is generally slower than that of DiI. DiO and DiI are often used together in dual color studies. Also see our Neuro-DiO (#60015).
  • ex\em(MeOH) = 484/501nm
  • e (MeOH) = 150,000
  • Yellow orange solid soluble in ethanol
  • Store at 4°C and protect from light, especially in solution.
  • C53H85ClN2O6.
  • Mwt: 882
  • [34215-57-1]

Chemical Structure of 60011

Reference:

1) Derzko, Z., et al. Biochemistry 19, 6050(1980);2) Leuther, M.D., et al. J. Immunology 127, 893(1981);3) Honig, M.G. and Hume, R.I. Trends in Neurosci. 9, 333(1989); 4) Honig, M.G. and Hume, R.I. J. Cell Biol. 103, 171(1986)
Catelog Number :   
    60012
Product Name :   
     DiOC14(3), hydroxyethanesulfonate (3,3'-ditetra decyloxacarbocyanine, hydroxyethanesulfonate)

Description:

DiOC14(3) is a DiO derivative, but is more soluble in aqueous media than the latter because of the shorter hydrocarbon chains. The dye is more suitable than DiO for staining cells suspension. An even better choice for staining cell suspension is our ready-to-use CellBriteTM Cell Membrane Labeling Kits in Bioscience Kits section
  • ex\em(MeOH) = 484/501nm
  • e(MeOH) = 150,000
  • Orange solid soluble in methanol, ethanol or DMSO
  • Store desiccated at 4°C
  • C47H74N2O6S
  • Mwt: 795

Chemical Structure of 60012

Reference:
Catelog Number :   
    60014
Product Name :   
     DiD (DiIC18(5) or 1,1'-dioctadecyl-3,3,3',3'- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt)

Description:

This lipophilic carbocyanine dye is similar to DiI, but has longer absorption and emission (ex\em= 644/663nm (MeOH) (e= 193,000)). DiR, DiD, DiI and Neuro-DiO can be used in combination for multicolor imaging.
  • ex\em(MeOH) = 644/ 655nm2
  • Dark blue solid soluble in ethanol, DMF and DMSO
  • Store at -20°C and protect from light.
  • C67H103ClN2O3S
  • Mwt: 1052.1

Chemical Structure of 60014

Reference:
Catelog Number :   
    60015
Product Name :   
     Neuro-DiO

Description:

Biotium developed Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate. Neuro-DiO and DiO have nearly identical absorption and emission spectra, but the former has better solubility in membranes and does not form nonfluorescent aggregates, which also tend to slow down the dye diffusion rate in membranes. Please also see our ready-to-use CellBriteTM Cell Membrane Labeling Kits for labeling cells in suspension in Bioscience Kits section
  • ex\em = 484/501nm
  • Yellow-orange solid soluble in ethanol, hexane, DMSO or vegetable oil
  • Store at -20°C
  • C67H105ClN2O5S
  • Mwt: 1086

Chemical Structure of 60015

Reference:

 

 
Catelog Number :   
    60016
Product Name :   
     Neuro-DiI

Description:

Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI. Neuro-DiI has structural features that may make the probe diffuse faster than DiI on cell membranes and also may result in a more stable labeling. Neuro-DiI has nearly identical absorption and emission wavelengths to those of DiI.
  • ex\em (MeOH) = 549/565 nm
  • e = 148,000
  • Red solid soluble in methanol, ethanol, DMF and DMSO
  • Store at 4°C and protect from light
  • C67H115IN2
  • Mwt: 1072

Chemical Structure of 60016

Reference:
Catelog Number :   
    60017
Product Name :   
     DiR (DiIC18(7) or 1,1'-dioctadecyl-3,3,3',3'-tetramethyl indotricarbocyanine Iodide)

Description:

DiR is similar to DiI and DiD, but has near IR absorption and emission. DiR, DiD, DiI and Neuro-DiO can be used in combination for multicolor imaging.
  • ex\em(MeOH) = 748/780 nm
  • e = 270,000
  • Dark blue-green oily solid soluble in ethanol, DMF or DMSO
  • Store at -20°C
  • C63H101IN2
  • Mwt: 1013.4

Chemical Structure of 60017

Reference:
Catelog Number :   
    60018
Product Name :   
     DiI in vegetable oil

Description:

DiI is uniformly dissolved in vegetable oil in a formulation optimized for microinjection. Carbocyanine microinjection technique has been widely used for fate mapping and cell lineage studies and for the determination of spatial organization and connectivity patterns of central nervous systems. The red fluorescent DiI and green fluorescent Neuro-DiO in oil can be used in combination for two color imaging.
  • Red oil
  • Store at room temperature and protect from light (Do not freeze)

Reference:

1) Ruiz, et al. “Fate maps and cell lineage analysis” in Essential developmental biology: a practical approach Oxford: IRL Press at Oxford University Press, 81-95(1993); 2) International Journal for Parasitology 28, 363(1998)
Catelog Number :   
    60019
Product Name :   
     Neuro-DiO in vegetable oil

Description:

Neuro-DiO is a derivative of DiO and has spectral property nearly identical to that of the latter. However, unlike DiO, Neuro-DiO is structurally more similar to DiI, which is known to be a much better lipophilic neuronal tracer than regular DiO. Like DiI in oil, Neuro-DiO is uniformly dissolved in vegetable oil in a formulation optimized for microinjection. The green fluorescent Neuro-DiO and red fluorescent DiI in oil can be used in combination for two color imaging.
  • ex\em= 484/501nm
  • Yellow-orange oil
  • Store at room temperature and protect from light (Do not freeze).
  • C67H105ClN2O5S
  • Mwt: 1086

Reference:
Catelog Number :   
    60034
Product Name :   
     Dilinoleyl DiI (also called FAST DiI1 or 1,1'-dilinoleyl-3,3,3',3'-tetramethylindocarbocynanine perchlorate)

Description:

Dilinoleyl DiI and DiI (#60010) have identical absorption and emission spectra. However, the unsaturated hydrocarbon chains of dilinoleyl DiI make the dye have a much faster lateral diffusion rate in cell membranes than regular DiI. This property makes dilinoleyl DiI particularly useful for tracing neurons in tissues.
  • ex\em(MeOH) = 549/565 nm
  • e = 148,000
  • Dark red oily paste soluble in ethanol, DMF or DMSO
  • Store at -20°C
  • C59H89ClN2O4
  • Mwt: 925.82

Chemical Structure of 60034

Reference:

1) trademark of Molecular Probes; 2) Neuroreport 8, 3965(1997); 3) Biophys. J. 70, 988(1996); 4) J. Cell Biol. 134, 1427(1996).
         
Catelog Number :   
    60035
Product Name :   
     Dilinoleyl DiO (also called FAST DiO1, or 1,1'-dilinoleyl-3,3'-oxacarbocynanine perchlorate)

Description:

Dilinoleyl DiO and DiO (#60010) have identical absorption and emission spectra. However, the unsaturated hydrocarbon chains of dilinoleyl DiO make the dye have a much faster lateral diffusion rate in cell membranes than regular DiO.
  • ex\em (MeOH) = 484/499 nm
  • e = 140,000
  • Yellow orange solid soluble in ethanol, DMF or DMSO
  • Store at -20°C
  • C53H77ClN2O6
  • Mwt: 873.65

Chemical Structure of 60035

Reference:

1) trademark of Molecular Probes; 2) Neuron 17, 91(1996)