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FURA-2 and Furaptra
product specification
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Catelog Number :
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50029 |
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Product Name :
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Fura-2, AM ester, 1mM in
anhydrous DMSO |
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Catelog Number :
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50030 |
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Product Name :
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Fura-2, pentaammonium salt |
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Description:
Membrane-impermeant but can be loaded into cells via
microinjection or scrape loadingK d: 224 nM.
lex\ lem
= 363nm/512nm; lex\ lem(high
Ca 2+) = 335nm/505nm. span
style='mso-char-type:symbol;mso-symbol-font-family:Symbol'>e
(363nm, no Ca2+) = 27,000 M-1cm-1;
e(335nm, high Ca2+) = 35,000 M-1cm-1.
Light yellow solid soluble in DMSO
Store at 4°C Protect from light, especially when in
solution. C29H37N8O14.
Mwt: 721. |
Chemical Structure of 50030
Reference:
Davis, M.J., et al. Am. J. Physiol. 263, 1292(1992);
Ward, C.A. and Moffat, M.P. J. Mol. Cell. Cardiol. 24, 937(1992). |
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Catelog Number :
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50031 |
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Product Name :
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Fura-2, pentapotassium salt |
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Description:
See information for
50030. C 29H 22K 5N 3O 14.
Mwt: 832. Structure similar to 50030. |
Reference:
Denk, W. et al. Science 248, 73(1990). |
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Catelog Number :
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50032 |
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Product Name :
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Fura-2, pentasodium salt |
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Description:
See information for
50030. C 29H 22N 3Na 5O 14.
Mwt: 752. [96314-98-6]. Structure similar to
50030 |
Reference:
Denk, W. et al. Science 248, 73(1990). |
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Catelog Number :
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50033 |
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Product Name :
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Fura-2, AM ester |
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Description:
Fura-2,AM ester is membrane-permeant and thus can be loaded
into cells via incubation. Because of the relatively low
water solubility of the AM ester, Pluronic F-127 (See
59000 and refs. Therein), a mild
detergent, is often used as a dispersing agent to facilitate
the loading. Fura-2/AM itself does not bind Ca 2+,
but it is readily hydrolyzed to Fura-2 by endogenous
esterases once the dye is inside the cells.
- Light yellow solid soluble in DMSO.
- Store at -20°C and protect from light, especially
when in solution.
- C44H47N3O24.
- Mwt: 1001.9.
- [108964-32-5].
For other information on Fura-2, see 50030. |
Chemical Structure of 50033
Reference:
Davis, M.J., et al. Am. J. Physiol. 263, 1292(1992);
Ward, C.A. and Moffat, M.P. J. Mol. Cell. Cardiol. 24, 937(1992). |
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Catelog Number :
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50033-1 |
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Product Name :
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Fura-2, AM ester |
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Description:
See # 50033
for product description. |
Chemical Structure of 50033-1
Reference: |
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Catelog Number :
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50034 |
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Product Name :
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Fura-2, AM ester |
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Description:
See # 50033
for product description. |
Chemical Structure of 50034
Reference: |
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Catelog Number :
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50035 |
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Product Name :
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Furaptra, tetrapotassium
salt (also known as Mag-Fura-2, tetrapotassium salt) |
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Description:
Mag-Fura-2 is a UV-excitable fluorescent indicator for
magnesium with a K d of 1.9 mM. 1,2
Similar to Fura-2, the excitation wavelength of Mag-Fura-2
undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2
also responds to Ca 2+ but with a significantly
lower K d than Fura-2 to Ca 2+. An
important application of Mag-Fura-2 is its use in detecting
high, transient Ca 2+ concentration during Ca 2+
spikes. 3,4 Mag-Fura-2/tetraammonium salt can be
loaded into cells by microinjection or scrape loading.
Light yellow solid soluble in water (pH
> 6). Store at -20°C and protect from light.
C18H10K4N2O11.
Mwt: 587. |
Chemical Structure of 50035
Reference:
1)Pro. Natl. Acad. Sci.USA 86,
2981(1989);
2) Am. J. Physiol. 256, C540(1989);
3) Neuron 10, 21(1993);
4) Biophys. J. 68, 2156(1995). |
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Catelog Number :
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50036 |
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Product Name :
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Furaptra, tetrasodium salt
(also known as Mag-Fura-2, tetrasodium salt) |
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Description:
Sodium salt of Mag-Fura-2 similar to
Mag-Fura-2/tetrapotassium salt ( 50035).
Light yellow solid soluble in water (pH > 6). Store at
-20°C and protect from light. C18H10N2Na4O11.
Mwt: 523. |
Reference:
1)Pro. Natl. Acad. Sci.USA 86,
2981(1989);
2) Am. J. Physiol. 256, C540(1989);
3) Neuron 10, 21(1993);
4) Biophys. J. 68, 2156(1995). |
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Catelog Number :
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50037 |
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Product Name :
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Furaptra, AM ester (also
known as Mag-Fura-2, AM ester) (special packaging) |
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Description:
Fura-2,AM ester is a membrane-permeant form of Furaptra. The
indicator is loaded into cells via simple incubation of
cells in the buffer containing the dye. See information for
Mag-Fura-2/tetrapotassium salt ( 50035).
Light yellow solid soluble in DMSO. Store at -20°C and
protect from light.
C30H30N2O19.
Mwt: 723. |
Chemical Structure of 50037
Reference: |
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Catelog Number :
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50038 |
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Product Name :
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Furaptra, AM ester (also
known as Mag-Fura-2, AM ester) |
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Description:
Fura-2,AM ester is a membrane-permeant form of Furaptra. The
indicator is loaded into cells via simple incubation of
cells in the buffer containing the dye. See information for
Mag-Fura-2/tetrapotassium salt ( 50035).
Light yellow solid soluble in DMSO. Store at -20°C and
protect from light.
C30H30N2O19.
Mwt: 723. |
Chemical Structure of 50038
Reference: |
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Catelog Number :
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50039 |
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Product Name :
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Furaptra, AM ester (also
known as Mag-Fura-2, AM ester) (special packaging) |
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Description:
Fura-2,AM ester is a membrane-permeant form of Furaptra. The
indicator is loaded into cells via simple incubation of
cells in the buffer containing the dye. See information for
Mag-Fura-2/tetrapotassium salt ( 50035).
Light yellow solid soluble in DMSO. Store at -20°C and
protect from light.
C30H30N2O19.
Mwt: 723. |
Chemical Structure of 50039
Reference: |
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Catelog Number :
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50045 |
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Product Name :
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Bis-Fura-2, hexapotassium
salt |
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Description:
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Bis-Fura-2, hexapotassium salt is membrane-impermeant
derivative of membrane-permeant calcium indicator Fura-2, AM
ester. It contains two Fura-2-pype fluorophores. This
indicator has a slightly lower infinity for Ca2+
and can be introduced into cell by microinjection.
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Reference:
1) J Neurophysiol 84,
2777(2000);
2) Brain Res 831,
113(1999);
3) J Neurophysiol 81,
2508(1999);
4) J Neurosci Res 57,
906(1999);
5) J. Neurochem Int 34,
391(1999)
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Catelog Number :
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59000 |
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Product Name :
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Pluronic F-127 (free sample
per order of AM esters) |
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Description:
Nonionic detergent useful for solubilizing large dye
molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and
Rhod-2/AM to facilitate cell loading. Pluronic F-127 is
usually dissolved in DMSO or water by gentle heating.
Pluronic F-127 is a registered trade mark of BASF. |
Reference:
Poenie, M. et al. Science 233,
886(1986);
Drummond, I.A.S., et al. J. Biol. Chem. 262, 12801(1987). |
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Catelog Number :
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59002 |
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Product Name :
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EDC (EDAC;
1-(3-dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) |
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Description:
EDC (or EDAC) has recently been found to be very useful for
fixing in situ chelators, including the fluorescent
ion indicators in this catalogue. The fixation of ion
indicators makes it useful for post histological studies
following the physiological experiments. EDC is also a
widely used reagent to activate carboxy groups for amine
couplings.
- White solid soluble in water.
- Store desiccated at -20°C.
- C8H18ClN3.
- Mwt: 191.7.
- [25952-53-8].
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Reference:
Tymianski, M. et al. Cell Calcium 21(3),
175(1997);
Tetrahedron Lett. 34, 7685(1993). |
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Catelog Number :
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59003 |
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Product Name :
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TPEN (Full name:
tetrakis-(2-pyridylmethyl)ethylenediamine) |
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Description:
TPEN is an excellent heavy metal chelator while it does not
affect Ca ++, Mg ++ , Na + or
K + concentrations. Thus, TPEN is a useful tool to
identify effects of heavy metals (Zn ++, Fe ++/Fe +++,
Cu ++, and Mn ++, etc.) on fluorescent
indicators of Ca ++, Mg ++ ,Na +
and K +.
- Off-white solid soluble in water, DMSO or DMF
- Store at –20°C
- C26H28N6
- Mwt: 425
- [16858-02-9]
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Reference:
J. Biol. Chem. 260, 2719(1985) |
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Catelog Number :
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59004 |
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Product Name :
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Pluronic F-127 20% solution
in DMSO |
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Description:
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This is a ready-to-use format of the dispersing reagent and
is particularly useful for loading moisture-sensitive
materials into cells.
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Reference: |
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Catelog Number :
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59005 |
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Product Name :
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Pluronic F-127, 10% in
sterile H2O |
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Description:
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This ready-to-use solution of the dispersing reagent is
useful for loading hydrophobic but moisture-sensitive
materials into cells.
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Reference: |
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Catelog Number :
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59007 |
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Product Name :
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Ionomycin,calcium salt |
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Description:
Ionomycin is commonly used to modify intracellular calcium
concentrations and to calibrate fluorescent calcium
indicators. It is also known for its antipoliferative
effects.
- Yellowish oily solid soluble in DMSO or EtOH
- Store desiccated at -20°C
- C41H70O9Ca
- Mwt: 747.1
- [56092-82-1]
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Reference:
1) J. Biol. Chem. 275, 7071(2000); 2) J. Immunol.
155, 3297 (1995) J. Urol. 162, 916 (1999). |
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Catelog Number :
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59100 |
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Product Name :
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Calcium Calibration
Buffer Kit (zero and 10 mM CaEGTA, 2x50mL) |
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Description:
This kit provides you a range of calibration buffers with
accurate calcium concentrations and is useful for the
calibration of fluorescent Ca 2+ indicators. 1,2
This product contains components A and B.
component A(zero free Ca2+):Zero mM
CaEGTA (10 mM K2EGTA,100mM KCI and 10mM MOPS;pH
7.20)
component B(40uM free Ca2+):10 mM
CaEGTA (10 mM CaEGTA,100mM KCI and 10mM MOPS;pH 7.20)
For detailed information on application,please obtain our
protocol.
Colorless liquid
Store desiccated at 4°C.
Kit to be used within three months after receipt
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Reference:
1)Cell Calcyium 12,279(1991);2)Neuropharmacology
34,1423(1995) |
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Catelog Number :
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90082 |
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Product Name :
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DMSO, anhydrous
(Dimethyl sulfoxide, or methyl sulfoxide) |
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Description:
Anhydrous DMSO is recommended for preparing stock solutions
of AM ester dyes, reactive dyes and other related
moisture-sensitive products.
- Colorless liquid at room temperature.
- Store desiccated at room temperature.
- C2H6OS
- Mwt: 78.13
- [67-68-5]
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Reference: |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45