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Enzyme Substrate Spec 2

Catelog Number :   
    10110-1
Product Name :   
     Coelenterazine (also known as Native coelenterazine)

Description:

See #10110 for product description.

Chemical Structure of 10110-1

Reference:
Catelog Number :   
    10110-2
Product Name :   
     Coelenterazine (also known as Native coelenterazine)

Description:

See #10110 for product description.

Chemical Structure of 10110-2
Catelog Number :   
    10110
Product Name :   
     Coelenterazine (also known as Native coelenterazine)

Description:

Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to >100 uM.1-4 Renilla luciferase, encoded by the luc gene, has been widely used as a repoter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer(BRET)5 to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.6-9
  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C26H21N3O3
  • Mwt: 423
  • [55779-48-1]

Chemical Structure of 10110

Reference:

1) Meth. Cell Biol. 40, 305(1994); 2) Meth. Enzymol. 172, 164(1989); 3) J. Cell Biol. 115, 1259(1991): 4) Cell Calcium, 14, 373 (1993); 5) Proc. Natl. Acad. Sci. USA 96, 151(1999); 6) Free Radic. Biol. Med. 28, 1232(2000); 7) Circ. Res. 84, 1203(1999); 8) Immunol. Today 15, 7(1994); 9) Anal. Biochem. 206, 273(1992); 10) Biochem. Biophys. Res. Commun. 233, 349(1997).
Catelog Number :   
    10112
Product Name :   
     Coelenterazine cp

Description:

Coelenterazine cp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 15 times higher and has a faster response time to calcium than native coelenterazine does. See Table 6.3 for comparison with other coelenterazine analogs.
  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C25H25N3O3
  • Mwt: 415.48

Reference:

1) Biochem. J. 261, 913(1989); 2) Cell Calcium 12, 635(1991); 3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10112-2
Product Name :   
     Coelenterazine cp

Description:

See #10112 for product description.
Catelog Number :   
    10112-1
Product Name :   
     Coelenterazine cp

Description:

See #10112 for product description.
Catelog Number :   
    10124
Product Name :   
     Coelenterazine e

Description:

Coelenterazine e is a synthetic derivative of coelenterazine, and is a superior substrate for Renilla luciferase. Coelenterazine e generates nearly 40% more light than native coelenterazine does while the initial light intensity of the former is more than 7 times higher than that of the latter.1 The emission spectrum of coelenterazine e/Renilla luciferase has two peaks centered at 418 and 475 nm, respectively (See Figure 6.3).

Coelenterazine e is also a good substrate for apoaequorin and, compared with coelenterazine (native), the e-aequorin complex formed from coelenterazine e has a faster luminescence rise time and shows two emission peaks at 405 and 465 nm respectively, with the ratio of the peak heights dependent on calcium concentration in the range of pCa 5-7.2 The dual emission makes coelenterazine e/apoaequorin possible to be used for calcium detection using the ratio of the two emission intensities.

  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C28H23N3O3
  • Mwt: 449.50

Chemical Structure of 10124

Reference:

1) ) Biochem. Biophys. Res. Commun. 233, 349(1997); 2) Biochem. J. 251, 405(1988).
Catelog Number :   
    10114
Product Name :   
     Coelenterazine f

Description:

Coelenterazine f is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is almost 20 times higher than that of the aequorin formed from native coelenterazine while its emission maximum is about 8 nm longer than that of the latter. See Table 6.3 for comparison.
  • Yellow solid soluble in MeOH or EtOH.
  • Store at -20°C and protect from light.
  • C26H20N3O2.
  • Mwt: 425.45

Chemical Structure of 10114

Reference:

1)Biochem. J. 261, 913(1989)
2)Cell Calcium, 12, 635(1991)
3)Cell Calcium, 14, 373 (1993)
Catelog Number :   
    10114-1
Product Name :   
     Coelenterazine f

Description:

See #10114 for product description.

Chemical Structure of 10114-1
Catelog Number :   
    10114-2
Product Name :   
     Coelenterazine f

Description:

See #10114 for product description.

Chemical Structure of 10114-2
Catelog Number :   
    10117-2
Product Name :   
     Coelenterazine fcp

Description:

See #10117 for product description.

Chemical Structure of 10117-2
Catelog Number :   
    10117-1
Product Name :   
     Coelenterazine fcp

Description:

See #10117 for product description.

Chemical Structure of 10117-1
Catelog Number :   
    10117
Product Name :   
     Coelenterazine fcp

Description:

Coelenterazine fcp is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is 135 times higher than that of the aequorin formed from native coelenterazine. See Table 6.3 for comparison.
  • Yellow solid soluble in MeOH.
  • Store at -20°C and protect from light.
  • C25H24FN3O2.
  • Mwt: 417.48

Chemical Structure of 10117

Reference:

1) Biochem. J. 261, 913(1989); 2) Cell Calcium 12, 635(1991); 3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10111-2
Product Name :   
     Coelenterazine h (2-(4-Dehydroxy) coelenterazine)

Description:

See #10111 for product description.

Chemical Structure of 10111-2
Catelog Number :   
    10111-1
Product Name :   
     Coelenterazine h (2-(4-Dehydroxy) coelenterazine)

Description:

See #10111 for product description.

Chemical Structure of 10111-1
Catelog Number :   
    10111
Product Name :   
     Coelenterazine h (2-(4-Dehydroxy) coelenterazine)

Description:

Coelenterazine h is a synthetic derivative of coelenterazine, but the luminescence intensity of its aequorin complex is more than 10 times higher than that of the aequorin formed from native coelenterazine. See Table 6.3 for comparison.
  • Yellow solid soluble in MeOH or EtOH.
  • Store at -20°C and protect from light.
  • C26H21N3O2.
  • Mwt: 407.
  • [50909-86-9]

Chemical Structure of 10111

Reference:

1) Biochem. J. 261, 913(1989); 2) Cell Calcium 12, 635(1991); 3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10113-1
Product Name :   
     Coelenterazine hcp

Description:

See #10113 for product description.

Chemical Structure of 10113-1
Catelog Number :   
    10113
Product Name :   
     Coelenterazine hcp

Description:

Coelenterazine hcp is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is 190 times higher than that of the aequorin complex formed from native coelenterazine while the response time to calcium is faster. See Table 6.3 for comparison.
  • Yellow solid soluble in MeOH or EtOH.
  • Store at -20°C and protect from light.
  • C25H25N3O2.
  • Mwt: 399.49

Chemical Structure of 10113

Reference:

1) Biochem. J. 261, 913(1989); 2) Cell Calcium 12, 635(1991); 3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10113-2
Product Name :   
     Coelenterazine hcp

Description:

See #10113 for product description.

Chemical Structure of 10113-2
Catelog Number :   
    10121
Product Name :   
     Coelenterazine i

Description:

Coelenterazine i is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is about 3% that of the aequorin formed from native coelenterazine and the response time to calcium is the slowest of all coelenterazine analogs. See Table 6.3 for comparison.
  • Yellow solid soluble in MeOH or EtOH.
  • Store at -20°C and protect from light.
  • C26H20IN3O2.
  • Mwt: 533.36

Reference:

1) Biochem. J. 261, 913(1989); 2) Cell Calcium 12, 635(1991); 3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10121-1
Product Name :   
     Coelenterazine i

Description:

See #10121 for product description.
Catelog Number :   
    10121-2
Product Name :   
     Coelenterazine i

Description:

See #10121 for product description.
Catelog Number :   
    10116
Product Name :   
     Coelenterazine ip

Description:

Coelenterazine ip is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is almost 50 times higher than that of the aequorin formed from native coelenterazine while its response time to calcium is much slower than the latter. See Table 6.3 for comparison.
  • Yellow solid soluble in MeOH or EtOH.
  • Store at -20°C and protect from light.
  • C23H23N3O3.
  • Mwt: 389.45.

Reference:

1) Biochem. J. 261, 913(1989);

2) Cell Calcium 12, 635(1991);
3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10116-2
Product Name :   
     Coelenterazine ip

Description:

See #10116 for product description.
Catelog Number :   
    10116-1
Product Name :   
     Coelenterazine ip

Description:

See #10116 for product description.
Catelog Number :   
    10115
Product Name :   
     Coelenterazine n

Description:

Coelenterazine n is a synthetic derivative of coelenterazine. The luminescence intensity of its aequorin complex is the weakest of all coelenterazine analogs and its response time to calcium is also much slower than that of native coelenterazine (See Table 6.3 for comparison). Coelenterazine n is reported to be a very useful low-sensitivity coelenterazine.
  • Yellow solid soluble in MeOH or EtOH.
  • Store at -20°C and protect from light.
  • C30H23N3O2.
  • Mwt: 457.52

Chemical Structure of 10115

Reference:

1) Biochem. J. 261, 913(1989); 2) Cell Calcium 12, 635(1991); 3) Cell Calcium, 14, 373 (1993).
Catelog Number :   
    10115-2
Product Name :   
     Coelenterazine n

Description:

See #10115 for product description.

Chemical Structure of 10115-2
Catelog Number :   
    10115-1
Product Name :   
     Coelenterazine n

Description:

See #10115 for product description.

Chemical Structure of 10115-1
Catelog Number :   
    10125-2
Product Name :   
     Coelenterazine 400a (also known as DeepBlue CTM)

Description:

See #10125 for product description.
Catelog Number :   
    10125
Product Name :   
     Coelenterazine 400a (also known as DeepBlue CTM)

Description:

Colenterazine 400a, also called DeepBlueC, is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor.
  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C26H21N3O
  • Mwt: 391.46

Reference:

1) Deep Blue C is a trademark of BioSignal Packard.
Catelog Number :   
    10125-1
Product Name :   
     Coelenterazine 400a (also known as DeepBlue CTM)

Description:

See #10125 for product description.

Reference:

1) Deep Blue C is a trademark of BioSignal Packard.
Catelog Number :   
    10124-2
Product Name :   
     Coelenterazine e

Description:

See #10124 for product description.

Chemical Structure of 10124-2

Reference:

1) Biochem. Biophys. Res. Commun. 233, 349(1997);

2) Biochem. J. 251, 405(1988).
Catelog Number :   
    10123
Product Name :   
     Coelenterazine Sampler Kit

Description:

For researchers wishing to screen for the appropriate coelenterazine derivatives to use, we offer this kit that contains 25ug each of nine coelenterazine analogs: coelenterazine, coelenterazine cp, coelenterazine f, coelenterazine fcp, coelenterazine h, coelenterazine hcp, coelenterazine i, coelenterazine ip, and coelenterazine n.
Catelog Number :   
    10122
Product Name :   
     Coelenterazine, 2-methyl analog (Methyl Coelenterazine)

Description:

Methyl coelenterazine has been reported to be a superior antioxidant for cells against reactive oxygen species (ROS) such as singlet oxygen and superoxide anion.1 The coelenterazine derivative is membrane-permeant, nontoxic and highly reactive toward ROS. As oxidative stress is believed to be a mediator of apoptosis,2 methyl coelenterazine should be another important tool for apopotosis study.
  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C20H17N3O2
  • Mwt: 331.37 Please also see our native coelenterazine (#10110) and other coelenterazine derivatives. Coelenterazine products have been used to detect superoxide and peroxynitrite via chemiluminescence.3,4

Reference:

1) Biochem. Pharmacol. 60, 471(2000); 2) Immunol. Today 15, 7(1994); 3)Anal. Biochem. 206, 273(1992); 4) Circ.Res. 84, 1203(1999).
Catelog Number :   
    10122-1
Product Name :   
     Coelenterazine, 2-methyl analog (Methyl Coelenterazine)

Description:

See #10122 for product description.

Reference:

 

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Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07