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Biotin Derivatives Product Specification

Catelog Number :

90073

Product Name :

ARP (N-(Aminooxyacetyl)-N'-(D-biotinoyl)hydrazine, trifluoroacetic acid salt)

Description:

ARP reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups.^1,2 The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes. ARP can freely cross the cell membranes, thus allowing detection of abasic sites in living cells.³ ARP is suitable for use in microplate assays.

White solid soluble in DMSO.
Store at -20°C.
C₁₄H₂₂F₃N₅O₆S.
Mwt: 445.41.

Chemical Structure of 90073

Reference:

1) Biochemistry 32, 8276-8283 (1993); 2) Biochemistry 31, 3703-3708 (1992); 3) Proc Natl Acad Sci U S A., 97, 686-691 (2000).

Catelog Number :

90055

Product Name :

Biocytin (e-biotinoyl ?-lysine)

Description:

Biocytin is a very useful cellular tracer that can be introduced into cells by microinjection.

White solid soluble in water or DMSO.
Stable at room temperature.
C₁₆H₂₈N₄O₄S.
Mwt: 372.48.
[576-19-2]

Chemical Structure of 90055

Reference:

1) Anat Embryol 199, 149 (1998); 2)Brain Res 782, 113 (1998); 3)Brain Res Devel Brain Res 106, 79 (1998); 4) Exp Brain Res 119, 375 (1998).

Catelog Number :

90060

Product Name :

Biocytin hydrazide

Description:

Biocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. Biocytin hydrazide can also be used to label glycoproteins or other carbohydrate molecules

White solid soluble in DMSO.
Store at 4°C
C₁₆H₃₀N₆O₃S.
Mwt = 386.51.
[102743-85-1]

Chemical Structure of 90060

Reference:

1) Glycoconjugate J. 9, 21(1992); 2) Anal Biochem 171, 1(1988); 3) Anal. Biochem. 170, 271 (1988); 4)Biochem. Biophys. Res. Comm. 136, 80(1986).

Catelog Number :

90079

Product Name :

Biotin cadaverine, free base (N-(5-aminopentyl)biotinamide)

Description:

Biotin cadaverine free base can be directly coupled to activated carboxylic acid groups without using an additional base. Also see biotin-X cadaverine free base (90080).

Chemical Structure of 90079

Reference:

Catelog Number :

90063

Product Name :

Biotin cadaverine, trifluoroacetate salt (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt)

Description:

Biotin cadaverine (90063), biotin-X cadaverine(90061), and biotin ethylenediamine(90057) can be coupled to carboxylic acids, DNA and other biomolecules.

White solid soluble in DMSO.
Store at 4°C
C₁₇H₂₉N₄O₄S.
Mwt = 442.5.

Chemical Structure of 90063

Reference:

1) J. Histochem. Cytochem. 38, 377(1990); 2) Annals NY Acad. Sci. 463, 214(1984); 3) Biochem. J. 251,935(1988).

Catelog Number :

90071

Product Name :

Biotin ethylenediamine (free base form)

Description:

Biotin ethylenediamine in free base form. White solid soluble in DMSO.

Store at -20°C.
C₁₂H₂₂N₄O₂S.
Mwt: 286.

Chemical Structure of 90071

Reference:

Catelog Number :

90059

Product Name :

Biotin ethylenediamine iodoacetamide (N-(biotinoyl)-N'-(iodoacetyl)ethylenediamine)

Description:

Biotin ethylenediamine iodoacetamide is readily coupled to thiols.

Off-white solid soluble in DMF.
Store at -20°C
C₁₄H₂₃IN₄O₃S.
Mwt = 454.33.

Chemical Structure of 90059

Reference:

1) J Biol Chem 274, 4722 (1999); 2) Biochem. J. 251, 935(1988).

Catelog Number :

90057

Product Name :

Biotin ethylenediamine, hydrobromide (equivalent to Neurobiotin^TM)

Description:

Biotin ethylenediamine, hydrobromide (identical to Neurobiotin) is a useful anterograde and transneuronal tracers.

White solid soluble in DMSO.
Store at -20°C
C₁₂H₂₃BrN₄O₂S.
Mwt: 367.

Chemical Structure of 90057

Reference:

1) J. Neurosci. 14, 3805((1994);
2) J. Neurosci. Meth. 41, 31(1992);
3) J. Neurosci.. Meth. 37, 141(1991).

Catelog Number :

90075

Product Name :

Biotin ethylenediamine, hydrochloride (functionally equivalent to Neurobiotin^TM)

Description:

Biotin ethylenediamine, hydrochloride is similar to biotin ethylenedimine, hydrobromide (90057).

Reference:

Catelog Number :

90074

Product Name :

Biotin NTA (biotin nitrilotriacetic acid, tripotassium salt; also called BNTA)

Description:

Biotin NTA is used to detect histidine-tagged proteins (for as little as less than 0.1 pmol) immobilized on nitrocellulose membranes. Biotin NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe.

White solid soluble in water
Store at 4°C
C₂₀H₃₆N₄O₈S
Mwt: 488.56

Chemical Structure of 90074

Reference:

1) Anal. Biochem. 236, 101 (1996); 2) Anal. Biochem. 229, 119 (1995).

Catelog Number :

90050

Product Name :

Biotin,succinimidyl ester (biotin SE)

Description:

Biotin SE is readily coupled to a primary or secondary amine under mild conditions.

White solid soluble in DMSO and moderately soluble in DMF.
Store at -20°C
C₁₄H₁₉N₃O₅S.
Mwt: 341.38

Chemical Structure of 90050

Reference:

1) Bioconjugate Chem 8, 552 (1997); 2) Bioconjugate Chem 8, 545 (1997); 3) J Immunol 159, 5871 (1997).

Catelog Number :

40035

Product Name :

Biotin-11-CTP, 10 mM in pH 7.5 Tris-HCl buffer (Biotin-11-cytidine-5'-triphosphate, tetralithium salt),

Description:

Biotin-11-CTP can be enzymatically incorporated into RNA

Colorless solution
Store at -20°C and protect from light
C₂₈H₄₂N₇O₁₇P₃SLi₄
Mwt: 901.42

Chemical Structure of 40035

Reference:

Catelog Number :

40036

Product Name :

Biotin-11-dCTP, 1 mM in pH 7.5 Tris-HCl buffer (Biotin-11-2'-deoxycytidine-5'-triphosphate, tetralithium salt),

Description:

Biotin-11-dCTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3'-end terminal labeling. The number ?11? is the number of atoms in the linker between biotin and dCTP.

Colorless solution
Store at -20°C and protect from light
C28H42N7O16P3SLi4
Mwt: 885.42

Chemical Structure of 40036

Reference:

Catelog Number :

40029

Product Name :

Biotin-11-dUTP, 1 mM in pH 7.5 Tris-HCl buffer (Biotin-11-2'-deoxyuridine-5'-triphosphate, tetralithium salt)

Description:

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3'-end terminal labeling. The number '11' is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-16-dUTP (40022 and 40022-1) and biotin-20-dUTP (40030 and 40030-1). The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, the longer the linker, the better biotin can interact with avidin or streptavidin.

Colorless solution

Store at -20°C and protect from light

C₂₈H₄₁N₆O₁₇P₃Sli₄

MWt: 886.5

Chemical Structure of 40029

Reference:

Catelog Number :

40029-1

Product Name :

Biotin-11-dUTP, lyophilized powder (Biotin-11-2'-deoxyuridine-5'-triphosphate, tetralithium salt),

Description:

This product is essentially the same as 40029 except that it is is a lyophilized solid that is more suitable for long term storage. The product contains lyophilized TE buffer, so you only need to add an appropriate amount of deionized H₂O to reconstitute the solution. For more product information, see 40029.

White solid soluble in H₂O

Store at -20°C and protect from light

C₂₈H₄₁N₆O₁₇P₃Sli₄

MWt: 886.5

Chemical Structure of 40029-1

Reference:

Catelog Number :

40033

Product Name :

Biotin-11-UTP, 10 mM in pH 7.5 Tris-HCl buffer (Biotin-11-uridine-5'-triphosphate, tetralithium salt)tetralithium salt)

Description:

Biotin-11-UTP can be enzymatically incorporated into RNA.

Colorless solution

Store at -20°C and protect from light

C₂₈H₄₁N₆O₁₈P₃SLi₄

Mwt: 902.5

Chemical Structure of 40033

Reference:

Catelog Number :

90083

Product Name :

Biotin-12 SE (4-((6-((biotinoyl)amino)hexanoyl)amino)butyric acid succinimidyl ester)

Description:

Biotin-12 SE is an amine-reactive biotin derivative with a long spacer group. This product can be conjugated to AA-dUTP or AA-UTP to make biotin-16-dUTP or biotin-16-UTP, two important products used in genomic studies.

White solid soluble in DMSO or DMF
Store at 4°C
C₂₄H₃₇N₅O₇S
Mwt: 539.64

Chemical Structure of 90083

Reference:

Catelog Number :

40022

Product Name :

Biotin-16-dUTP, 1 mM in pH 7.5 Tris-HCl buffer (Biotin-16-2'-deoxyuridine-5'-triphosphate, tetralithium salt)

Description:

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling.¹ The terminal deoxynucleotidyl transferase (TDT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies.^3,4 The number '16' is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-11-dUTP (#40029 and #40029-1) and biotin-20-dUTP (#40030 and #40030-1). The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, the longer the linker, the better biotin can interact with avidin or streptavidin.

Colorless solution
Store at -20°C and protect from light
C₃₂H₄₈N₇O₁₈P₃Sli₄
MWt: 971.5

Chemical Structure of 40022

Reference:

1) Anal. Biochem. 193, 2(1991); 2) J. Cell. Biol. 119, 493(1992); 3) NeuroReport, 7, 61(1995); 4) Biotechniques 19, 800(1995)

Catelog Number :

40022-1

Product Name :

Biotin-16-dUTP, lyophilized powder (Biotin-16-2'-deoxyuridine-5'-triphosphate, tetralithium salt)

Description:

This product is essentially the same as 40022 except that it is is a lyophilized solid that is more suitable for long term storage. The product contains lyophilized TE buffer, so you only need to add an appropriate amount of deionized H₂O to reconstitute the solution. For more product information, see 40022.

White solid soluble in H₂O
Store at -20°C and protect from light
C₃₂H₄₈N₇O₁₈P₃Sli₄
Mwt: 971.5

Chemical Structure of 40022-1

Reference:

Catelog Number :

40023

Product Name :

Biotin-16-UTP, 10 mM in pH 7.5 Tris-HCl buffer (Biotin-16-uridine-5'-triphosphate, tetralithium salt),

Description:

Biotin-16-UTP can be enzymatically incorporated into RNA.

Colorless solution
Store at -20°C and protect from light
C₃₂H₄₈N₇O₁₉P₃Sli₄
MWt: 987.51

Chemical Structure of 40023

Reference:

Catelog Number :

40030

Product Name :

Biotin-20-dUTP, 1 mM in pH 7.5 Tris-HCl buffer (Biotin-20-2′-deoxyuridine-5′-triphosphate,tetralithium salt)

Description:

This product is similar to biotin-11-dUTP (40029) and biotin-16-dUTP (40022) except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Colorless solution
Store at -20°C and protect from light
C₃₃H₅₁N₆O₂₁P₃SLi₄
Mwt: 1020.54

Chemical Structure of 40030

Reference:

Catelog Number :

40030-1

Product Name :

Biotin-20-dUTP, lyophilized powder (Biotin-20-2'-deoxyuridine-5'-triphosphate, tetralithium salt)

Description:

This product is essentially the same as 40030 except that it is is a lyophilized solid that is more suitable for long term storage. The product contains lyophilized TE buffer, so you only need to add an appropriate amount of deionized H₂O to reconstitute the solution. For more product information, see 40030.

White solid soluble in H₂O
Store at -20°C and protect from light
C₃₃H₅₁N₆O₂₁P₃SLi₄
Mwt: 1020.54

Chemical Structure of 40030-1

Reference:

Catelog Number :

40034

Product Name :

Biotin-20-UTP, 10 mM in pH 7.5 Tris-HCl buffer (Biotin-20-uridine-5'-triphosphate, tetralithium salt)

Description:

This product is similar to biotin-11-UTP (40033 ) and biotin-16-UTP (40023 ) except that it has a longer and watersoluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Colorless solution

Store at -20°C and protect from light

C₃₃H₅₁N₆O₂₂P₃SLi₄

Mwt: 1036.26

Chemical Structure of 40034

Reference:

Catelog Number :

90062

Product Name :

Biotin-4-fluorescein

Description:

Biotin-4-fluorescein has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biofluids. The biotin binding to avidin and streptavidin is high affinity, fast and noncooperative. Also see Fluorescein biotin (80019 ). Please also see biotin-rhodamine 110 (80022 ) for superior photostability.

l_ex\l_em = 494/523 nm.
Yellow solid soluble in DMSO, or pH>7 buffers.
Store at -20°C and protect from light.
C₃₃H₃₂N₄O₈S.
Mwt. = 644.7

Chemical Structure of 90062

Reference:

1)Biochim Biophys Acta 1427, 33 (1999); 2) Biochim Biophys Acta 1427, 44 (1999).

Catelog Number :

90078

Product Name :

Biotin-PEO₂-PPO₂-amine, trifluoroacetic acid salt

Description:

Biotin-PEO₂-PPO₂-amine is a water soluble biotin derivative with an amine group.

White solid soluble in water or DMSO
Store at -20°C
C₂₂H₃₉N₄O₇S
Mwt: 560.63

Chemical Structure of 90078

Reference:

Catelog Number :

90067

Product Name :

Biotin-PEO₃-amine

Description:

Biotin-PEO₃-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

White solid soluble in water or DMSO.
Store at -20°C.
C₁₆H₃₀N₄O₄S.
Mwt: 374.5.

Chemical Structure of 90067

Reference:

Biochemistry 21, 978(1982).

Catelog Number :

90068

Product Name :

Biotin-PEO₄-amine

Description:

Biotin-PEO₄-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

White solid soluble in water or DMSO.
Store at -20°C.
C₂₀H₃₅F₃N₄O₇S.
Mwt: 532.58.

Chemical Structure of 90068

Reference:

Biochemistry 21, 978(1982).

Catelog Number :

90111

Product Name :

Biotin-PEO₄-hydrazide

Description:

Biotin-PEO₄-hydrazide is a water-soluble biotin derivative capable of reacting with aldehydes or ketones to form hydrazones.

White solid soluble in water.
Store at 4°C.
C₂₁H₃₉N₅O₇S.
Mwt: 505.63.

Chemical Structure of 90111

Reference:

Catelog Number :

90112

Product Name :

Biotin-PEO₄-maleimide

Description:

Biotin-PEO₄-maleimide is a water soluble biotin derivative that can be readily coupled to thiols.

White solid soluble in water or DMSO
Store at -20°C
C₂₇H₄₃N₅O₈S
Mwt: 597.28

Chemical Structure of 90112

Reference:

Catelog Number :

90069

Product Name :

Biotin-PEO₄-Propionate Succinimidyl Ester

Description:

Biotin-PEO₄-propionate succinimidyl ester is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.

White solid soluble in water or DMSO.
Store at -20°C.
C₂₅H₄₀N₄O₁₀S.
Mwt: 588.7.

Chemical Structure of 90069

Reference:

Catelog Number :

80022

Product Name :

Biotin-rhodamine 110

Description:

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biofluids. In addition, biotin-rhodamine 110 can be used as a polar tracer to study the morphology of cells, similar to the use of Lucifer Yellow cadaverine biotin-X ( 80017). The dye rhodamine 110 (or carboxyrhodamine 110) has absorption and emission wavelengths similar to those of fluorescein (See Figures 7.4 and 7.5). However, the spectra and fluorescent quantum yield of rhodamine 110 are relatively unaffected by pH change (pH 4-9), whereas the fluorescence of fluorescein is significantly reduced at acidic pH. Moreover, rhodamine 110 is much more photostable than fluorescein (See Figure 7.6), making biotin-rhodamine 110 a better choice for studies where prolonged exposure to light may be necessary.

l_ex\l_em(MeOH) = 502/524 nm
Orange red solid soluble in DMF or DMSO
Store at -20°C and protect from light, especially in solution
C₄₁H₅₀N₆O₉S
Mwt: 802.94

Chemical Structure of 80022

Reference:

Catelog Number :

90080

Product Name :

Biotin-x cadaverine, free base (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, free base)

Description:

Biotin-X cadaverine can be coupled directly to activated carboxylic acid esters without the use of a base.

White solid soluble in DMSO
Store at -20°C
C₂₁H₃₉N₅O₃S
Mwt: 441.63

Chemical Structure of 90080

Reference:

Catelog Number :

90061

Product Name :

Biotin-X cadaverine, trifluoroacetate salt (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, trifluoroacetic acid salt)

Description:

Biotin-X cadaverine trifluoroacetic acid salt can be coupled to activated carboxylic acid esters or sulfonyl chlorides in the presence of a base or in a basic buffer. Also see biotin-X cadaverine free base (90080).

Tan solid soluble in DMSO
Store at -20°C
White solid soluble in DMSO.
Store at -20°C
C₂₃H₄₀F₃N₅O₅S.
Mwt = 555.65.

Chemical Structure of 90061

Reference:

1) J. Histochem. Cytochem. 38, 377(1990); 2) Annals NY Acad. Sci. 463, 214(1984); 3) Biochem. J. 251, 935(1988).

Catelog Number :

90056

Product Name :

Biotin-X NTA (or Biotin-X nitrilotriacetic acid, tripotassium salt)

Description:

Biotin-X NTA is used to detect histidine-tagged proteins (for as little as less than 0.1 pmol) immobilized on nitrocellulose membranes. Biotin-X NTA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe.

White solid soluble in water.
Store at 4°C
C₂₆H₄₀K₃N₅O₉S.
Mwt: 715.98

Chemical Structure of 90056

Reference:

1) Anal. Biochem. 236, 101 (1996); 2) Anal. Biochem. 229, 119 (1995).

Catelog Number :

90053

Product Name :

Biotin-X, free acid (6-((biotinoyl)amino)hexanoic acid)

Description:

Biotin-X free acid can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

White solid soluble in DMSO and moderately soluble in DMF.
Stable at room temperature.
C₁₆H₂₇N₃O₄S.
Mwt: 357.47.
[72040-64-3]

Chemical Structure of 90053

Reference:

1) Anal Chem 67, 1014 (1995);
2) A detailed protocol for making mixed anhydrides is provided upon request with purchase of the product.

Catelog Number :

90051

Product Name :

Biotin-X, succinimidyl ester (6-((biotinoyl)amino)hexanoic acid succinimidyl ester)

Description:

Biotin-X succinimidyl ester is readily coupled to a primary or secondary amine under mild conditions.

White solid soluble in DMSO and moderately soluble in DMF.
Store at -20°C
C₂₀H₃₀N₄O₆S.
Mwt: 454.54.
[72040-63-2]

Chemical Structure of 90051

Reference:

1) J Biol Chem 274, 21878 (1999);2) J Biol Chem 273, 793 (1998); 3) Meth Mol Biol 80, 173 (1998).

Catelog Number :

90058

Product Name :

Biotin-X-C₅-maleimide (N-(5-(6-((biotinoyl)amino)hexanoyl)amino)pentylmaleimide)

Description:

Biotin-X-C₅-maleimide is readily and selectively coupled to thiols.

White solid soluble in DMSO, or DMF.
Store at -20°C
C₂₇H₃₉N₅O₇S.
Mwt. = 521.67.

Chemical Structure of 90058

Reference:

Catelog Number :

90054

Product Name :

Biotin-XX, free acid (6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoic acid)

Description:

Biotin-XX free acid can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

White solid soluble in DMSO and moderately soluble in DMF.
Stable at room temperature.
C₂₂H₃₈N₄O₅S.
Mwt: 470.

Chemical Structure of 90054

Reference:

A detailed protocol for making mixed anhydrides is provided upon request with purchase of the product.

Catelog Number :

90076

Product Name :

Biotin-XX, SSE (6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoic acid sulfosuccinimidyl ester)

Description:

Biotin-XX SSE is a water-soluble, amine-reactive biotin derivative. The long XX arm facilitates interaction between biotin and avidin or streptavidin.

White solid soluble in water and moderately soluble in DMSO
Store at -20°C
C₂₆H₄₀N₅NaO₁₀S₂
Mwt: 669.74

Chemical Structure of 90076

Reference:

1)J Biol Chem 267, 5847 (1992).

Catelog Number :

90052

Product Name :

Biotin-XX, succinimidyl ester (6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester)

Description:

Biotin-XX succinimidyl ester is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.

White solid soluble in DMSO and moderately soluble in DMF.
Store at -20°C.
C₂₆H₄₁N₅O₇S.
Mwt: 567.7.
[89889-52-1]

Chemical Structure of 90052

Reference:

1) Bioconjugate Chem 10, 32 (1999); 2) J Cell Biol 144, 99 (1999); 3) Anal. Chem. 70, 1242 (1998); 4) Meth Mol Biol 80, 173 (1998).

Catelog Number :

00017

Product Name :

Biotin-XX-a-Bungarotoxin

Description:

Biotinylated a-bungarotoxin is useful in the affinity column isolation of the nicotinic AChR using an avidin or streptavidin agarose.¹ Nicotinic AChRs labeled with biotin-XX-a-bungarotoxin can be localized using enzymeor fluorophore-labeled conjugates of avidin or streptavidin.^2,3

White lyophilized solid soluble in water
Store at -20°C and protect from light
Mwt: ~8400

Reference:

1) J Cell Biol 125, 661-668 (1994); 2) J Biol Chem 268, 25108(1993); 3) Proc Natl Acad Sci U S A 77, 4823(1980)

Catelog Number :

90072

Product Name :

D(+)-Biotin

Description:

White solid soluble in DMSO or DMF

Store at 4°C

C₁₀H₁₆N₂O₃S

Mwt: 244.31

[58-85-5]

Chemical Structure of 90072

Reference:

Catelog Number :

51022

Product Name :

Flubida-2

Description:

Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidinchimera proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by endoesterases. Flubida-2 is membrane-permeable and thus can be delivered into cells via simple incubation with the probe in a buffer.

Off-white solid soluble in DMSO
Store at 4°C
C₄₃H₄₈N₄O₉S
Mwt: 812.93

Chemical Structure of 51022

Reference:

Chemistry & Biology 7, 197(2000).

Catelog Number :

80019

Product Name :

Fluorescein biotin (5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein)

Description:

Fluorescein biotin can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins. Also see biotin-rhodamine 110 (80022) and biotin-4-fluorescein (90062) in Section 8.

l_ex\l_em= 494/518 nm
Yellow solid soluble in DMSO, or DMF
Store at -20°C and protect from light
C₄₂H₅₀N₆O₈S₂
Mwt: 831
[134759-22-1]

Chemical Structure of 80019

Reference:

1) Biochim Biophys Acta 1381, 203 (1998);
2) Clin.Chem. 40, 2112(1994).

Catelog Number :

80017

Product Name :

Lucifer Yellow Cadaverine Biotin-X, dipotassium salt

Description:

This fluorescent biotin derivative is a very useful tracer for studying neuronal morphology. The biotin portion of the molecule allows researchers to amplify the fluorescent signal using standard biotin-avidin or biotin-streptavidin biochemistry. The staining can tolerate conditions of aldehyde fixation and sectioning, and washing with detergent.

l_ex\l_em= 428/532 nm.
Yellow solid soluble in water.
Store at -20°C and protect from light.
C₃₃H₄₂K₂N₆O₁₁S₃.
Mwt: 873.

Chemical Structure of 80017

Reference:

1) J. Neurosci. Meth. 53, 23(1994);2) J. Neurosci. Meth. 46, 59(1993).

Catelog Number :

90064

Product Name :

MTSEA biotin (2-((biotinoyl)amino)ethyl methanethiosulfonate)

Description:

MTSEA biotin selectively labels thiols under mild condition. Please also see our TS biotin ethylenediamine (#92010), TS biotin-X ethylenediamine (#92011) and TS biotin- XX ethylenediamine (#92012), all of which allow selective labeling of cell surface thiols.

White solid soluble in DMF, or DMSO at =10 mg/mL
Store desiccated at -20°C
C₁₃H₂₃N₃O₄S₃
Mwt: 381.52

Chemical Structure of 90064

Reference:

1) J Membr Biol, 180(1), 83 (2001); 2) J Neurosci, 21(1), 67 (2001); 3) J Biol Chem, 2000 Oct 31; 4) J Neurosci, 19(12), 4847 (1999); 5) J Biol Chem, 273(20), 12675 (1998)

Catelog Number :

90064-1

Product Name :

MTSEA biotin (2-((biotinoyl)amino)ethyl methanethiosulfonate)

Description:

See #91064 for product description.

Chemical Structure of 90064-1

Reference:

Catelog Number :

90065-1

Product Name :

MTSEA biotin-X (MTSEA BIOTINCAP, or 2-((6-((biotinoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate))

Description:

See #90065 for product description.

Chemical Structure of 90065-1

Reference:

Catelog Number :

90065

Product Name :

MTSEA biotin-X (MTSEA BIOTINCAP, or 2-((6-((biotinoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate))

Description:

MTSEA biotin-X selectively labels thiols under mild condition. The X-spacer should facilitate biotin-avidin interaction. Please also see TS biotin ethylenediamine (#92010), TS biotin-X ethylenediamine (#92011) and TS biotin-XX ethylenediamine (#92012), all of which allow selective labeling of cell surface thiols.

White solid soluble in DMF, or DMSO at =10 mg/mL
Store desiccated at -20°C
C₁₉H₃₄N₄O₅S₃
Mwt: 494.68

Chemical Structure of 90065

Reference:

1) J Membr Biol, 180(1), 83 (2001); 2) J Neurosci, 21(1), 67 (2001); 3) J Biol Chem, 2000 Oct 31; 4) J Neurosci, 19(12), 4847 (1999); 5) J Biol Chem, 273(20), 12675 (1998)

Catelog Number :

90066

Product Name :

MTSEA biotin-XX (MTSEA BIOTINCAPCAP, or 2-((6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate)

Description:

O MTSEA biotin-XX selectively labels thiols under mild condition. The XX-linker should facilitate biotin-avidin interaction. Please also see TS biotin ethylenediamine (#92010), TS biotin-X ethylenediamine (#92011) and TS biotin-XX ethylenediamine (#92012), all of which allow selective labeling of cell surface thiols.

White solid soluble in DMF, or DMSO at =10 mg/mL
Store desiccated at -20°C
C₂₅H₄₅N₅O₆S₃
Mwt: 607.7

Chemical Structure of 90066

Reference:

1) J Membr Biol, 180(1), 83 (2001); 2) J Neurosci, 21(1), 67 (2001); 3) J Biol Chem, 2000 Oct 31; 4) J Neurosci, 19(12), 4847 (1999); 5) J Biol Chem, 273(20), 12675 (1998)

Catelog Number :

90066-1

Product Name :

MTSEA biotin-XX (MTSEA BIOTINCAPCAP, or 2-((6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate)

Description:

See #90066 for product description.

Chemical Structure of 90066-1

Reference:

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Biotin Derivatives Product Specification

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Copyright © 2005 Gentaur BVBA
Last modified: feb-07