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Apoptosis Assays
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Catelog Number :
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30017 |
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Product Name :
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Apoptosis &
Necrosis Quantification Kit |
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Kit Components:
- FITC-Annexin V
- Ethidium homodimer III
- 5X binding buffer
Description:
Apoptosis and necrosis are two major
processes by which cells die. Apoptosis is an active,
genetically regulated disassembly of the cell from within.
Disassembly creates changes in the phospholipid content of
the cytoplasmic membrane outer leaflet. Phosphatidylserine
(PS) is translocated from the inner to the outer surface of
the cell for phagocytic cell recognition. The human
anticoagulant, annexin V, is a 35 kD Ca2+-dependent
phospholipid protein with a high affinity for PS. Annexin V
labeled with fluorescein (FITC) (labs/lem=
492/ 514 nm) can identify apoptotic cells in green by
binding to PS exposed on the outer leaflet. Necrosis
normally results from a severe cellular insult. Both
internal organelle and plasma membrane integrity are lost,
resulting in spilling of cytosolic and organellar contents
into the surrounding environment. Ethidium homodimer III
(EtD-III) is a highly positivelycharged nucleic acid probe,
which is impermeant to live cells or apoptotic cells, but
stains necrotic cells with red fluorescence (labs/lem(with
DNA) = 528/617 nm). EthD-III is a superior alternative to
propidium iodide (PI) or ethidium homodimer I used in some
of our competitors?kits because of its significantly higher
affinity for DNA and higher fluorescence quantum yield.
Apoptosis & Necrosis Quantification Kit provides a
convenient assay for quantifying apoptotic (green) and
necrotic (red) cells within the same cell population by flow
cytometry or fluorescence microscopy.
Features:
- Simple: Simultaneous detections of both apoptotic
and necrotic cells in a single assay.
- Fast: A single 15-30 min staining step.
- Sensitive: EthD-III is a superior alternative to
both PI and EthD-I for staining necrotic cells.
Spectra
For spectra of fluorescein and EthD-III.
Related Products:
- JC-1 Mitochondrial Membrane Potential Detection Kit
- Staurosporine
- Apoptotic, Necrotic & Healthy Cells Quantification Kit
Also see our extensive list of fluorogenic and luminogenic
caspase substrates in Section 6F.
Reference:
1) FEBS Lett. 203, 99(1986); 2) Biosci.
Rep. 7, 289(1987). |
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Catelog Number :
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30018 |
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Product Name :
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Apoptotic, Necrotic
& Healthy Cells Quantification Kit |
|
Kit Components:
- FITC-Annexin V
- Ethidium Homodimer III
- Hoechst 33342
- 5X binding Buffer
Description:
Apoptosis and necrosis are two major
processes by which cells die. Apoptosis is an active,
genetically regulated disassembly of the cell from within.
Disassembly creates changes in the phospholipid content of
the cytoplasmic membrane outer leaflet. Phosphatidylserine
(PS) is translocated from the inner to the outer surface of
the cell for phagocytic cells recognition. The human
anticoagulant, annexin V, is a 35 kD Ca2+-dependent
phospholipids protein with a high affinity for PS. Annexin V
labeled with a fluorophore such as FITC (fluorescein) can
identify apoptotic cells by binding to PS exposed on the
outer leaflet. Necrosis normally results from a severe
cellular insult. Both internal organelle and cytoplasmic
membrane integrity are lost, resulting in spilling of
cytosolic and organellar contents into the surrounding
environment. Our ethidium homodimer III (EthD-III) is a
highly charged nucleic acid probe, which is impermeant to
live cells and apoptotic cells but stains necrotic cells
with red fluorescence (labs/lem(with
DNA) = 528/617 nm). EthD-III is a superior alternative to
propidium iodide (PI) or ethidium homodimer I used in some
of our competitors'kits because of its significantly higher
affinity for DNA and higher fluorescence quantum yield. The
kit also includes a membrane-permeant blue fluorescent DNA
dye Hoechst 33342 (labs/lem(with
DNA) = 350/461nm) for quantifying healthy cell population
(3,4). The kit allows simultaneous quantification
of apoptotic (green), necrotic (red) and healthy cells
(blue) within the same cell population by flow cytometry or
fluorescence microscopy.
Features:
- Simple: Simultaneous detections of apoptotic,
necrotic and healthy cells in a single assay.
- Fast: A single 15-30 min staining step.
- Sensitive: EthD-III is a superior alternative to
both PI and EthD-I for staining necrotic cells.
Spectra:
For spectra of fluorescein, EthD-III and Hoechst 33342
Related Products:
- JC-1 Mitochondrial Membrane Potential Detection Kit
- Staurosporine
- MCB Glutathione Detection Kit
Also see our extensive list of fluorogenic and luminogenic
caspase substrates.
Reference:
1) FEBS Lett. 203, 99(1986); 2) Biosci.
Rep. 7, 289(1987); 3)Science 300, 91(2003); 4) J Neurosci
Methods. 36, 229(1991). |
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Catelog Number :
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30010-3 |
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Product Name :
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Caspase-3
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-DEVD-amino-D-luciferin
- Enzyme inhibitor Ac-DEVD-CHO
Description:
Caspase-3 is a cell-death protease
involved in the execution phase of apoptosis (1,2).
Caspase-3 Luminescent Assay Kit provides a homogenous assay
system for fast and ultra-sensitive detection of caspase-3
activity in purified enzyme or mammalian cell systems. This
kit utilizes Ac- DEVD-amino-D-luciferin in combination with
Firefly luciferase to detect caspase-3 activity. Upon
substrate cleavage at the Cterminal side of the DEVD peptide
sequence by caspase-3, amino-D-luciferin is released.
Amino-D-luciferin is a synthetic derivative of the natural
luciferase substrate D-luciferin and is also accepted by the
enzyme as a substrate, capable of generating bioluminescence
in the presence of oxygen, ATP and Mg2+. Thus,
amino-D-luciferin released from the caspase-3 activity is
subsequently oxidized via Firefly luciferase activity with
emission of light, the intensity of which is proportional to
the caspase-3 activity.
The history of detecting peptidase activities via
bioluminescence goes back a decade ago when Monsees and
colleagues first demonstrated the principle of coupling
peptidase activity to Firefly luciferase activity by the use
of amino-D-luciferin (3,4). The authors showed
that virtually any bioluminogenic peptidase substrates could
be made by conjugating a known peptidase-recognition amino
acid or peptide sequence to the amine group of
amino-D-luciferin, a method similar to how the widely used
chromogenic and fluorogenic peptidase substrates are
constructed. Therefore, considering the superior sensitivity
of bioluminescence-based detections, we have developed a
line of caspase and other peptidase substrates using the
same peptide sequences used in our chromogenic and
fluorogenic caspase substrates (See Assay Principle below).
Under optimal conditions, it is possible to detect <1 ng of
a peptidase by using a bioluminogenic substrate.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
Staurosporine Caspase-3 DEVD-R110
Fluorometric HTS Assay Kit
Caspase-3 DEVD-R110 Fluorometric and
Colorimetric Assay Kit (#30009-1 and #30009-2)
Reference:
1) Cell Death Diff. 6, 99(1999); 2) J.
Biol. Chem.. 274, 11549(1999); 3) Anal. Biochem. 221, 329(1994);
4) J. Am. Chem. Soc. 88, 2015(1966); |
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|
Catelog Number :
|
|
|
30010-1 |
|
|
Product Name :
|
|
|
Caspase-3
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-DEVD-amino-D-luciferin
- Enzyme inhibitor Ac-DEVD-CHO
Description:
Caspase-3 is a cell-death protease
involved in the execution phase of apoptosis (1,2).
Caspase-3 Luminescent Assay Kit provides a homogenous assay
system for fast and ultra-sensitive detection of caspase-3
activity in purified enzyme or mammalian cell systems. This
kit utilizes Ac- DEVD-amino-D-luciferin in combination with
Firefly luciferase to detect caspase-3 activity. Upon
substrate cleavage at the Cterminal side of the DEVD peptide
sequence by caspase-3, amino-D-luciferin is released.
Amino-D-luciferin is a synthetic derivative of the natural
luciferase substrate D-luciferin and is also accepted by the
enzyme as a substrate, capable of generating bioluminescence
in the presence of oxygen, ATP and Mg2+. Thus,
amino-D-luciferin released from the caspase-3 activity is
subsequently oxidized via Firefly luciferase activity with
emission of light, the intensity of which is proportional to
the caspase-3 activity.
The history of detecting peptidase activities via
bioluminescence goes back a decade ago when Monsees and
colleagues first demonstrated the principle of coupling
peptidase activity to Firefly luciferase activity by the use
of amino-D-luciferin (3,4). The authors showed
that virtually any bioluminogenic peptidase substrates could
be made by conjugating a known peptidase-recognition amino
acid or peptide sequence to the amine group of
amino-D-luciferin, a method similar to how the widely used
chromogenic and fluorogenic peptidase substrates are
constructed. Therefore, considering the superior sensitivity
of bioluminescence-based detections, we have developed a
line of caspase and other peptidase substrates using the
same peptide sequences used in our chromogenic and
fluorogenic caspase substrates (See Assay Principle below).
Under optimal conditions, it is possible to detect <1 ng of
a peptidase by using a bioluminogenic substrate.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
Staurosporine (#00025) Caspase-3 DEVD-R110
Fluorometric HTS Assay Kit (#30009-3, #30009-4 and #30009-5)
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
(#30009-1 and #30009-2)
Reference:
1) Cell Death Diff. 6, 99(1999); 2) J.
Biol. Chem.. 274, 11549(1999); 3) Anal. Biochem. 221, 329(1994);
4) J. Am. Chem. Soc. 88, 2015(1966); |
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|
Catelog Number :
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30010-2 |
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|
Product Name :
|
|
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Caspase-3
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-DEVD-amino-D-luciferin
- Enzyme inhibitor Ac-DEVD-CHO
Description:
Caspase-3 is a cell-death protease
involved in the execution phase of apoptosis (1,2).
Caspase-3 Luminescent Assay Kit provides a homogenous assay
system for fast and ultra-sensitive detection of caspase-3
activity in purified enzyme or mammalian cell systems. This
kit utilizes Ac- DEVD-amino-D-luciferin in combination with
Firefly luciferase to detect caspase-3 activity. Upon
substrate cleavage at the Cterminal side of the DEVD peptide
sequence by caspase-3, amino-D-luciferin is released.
Amino-D-luciferin is a synthetic derivative of the natural
luciferase substrate D-luciferin and is also accepted by the
enzyme as a substrate, capable of generating bioluminescence
in the presence of oxygen, ATP and Mg2+. Thus,
amino-D-luciferin released from the caspase-3 activity is
subsequently oxidized via Firefly luciferase activity with
emission of light, the intensity of which is proportional to
the caspase-3 activity.
The history of detecting peptidase activities via
bioluminescence goes back a decade ago when Monsees and
colleagues first demonstrated the principle of coupling
peptidase activity to Firefly luciferase activity by the use
of amino-D-luciferin (3,4). The authors showed
that virtually any bioluminogenic peptidase substrates could
be made by conjugating a known peptidase-recognition amino
acid or peptide sequence to the amine group of
amino-D-luciferin, a method similar to how the widely used
chromogenic and fluorogenic peptidase substrates are
constructed. Therefore, considering the superior sensitivity
of bioluminescence-based detections, we have developed a
line of caspase and other peptidase substrates using the
same peptide sequences used in our chromogenic and
fluorogenic caspase substrates (See Assay Principle below).
Under optimal conditions, it is possible to detect <1 ng of
a peptidase by using a bioluminogenic substrate.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
Staurosporine (#00025) Caspase-3 DEVD-R110
Fluorometric HTS Assay Kit (#30009-3, #30009-4 and #30009-5)
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
(#30009-1 and #30009-2)
Reference:
1) Cell Death Diff. 6, 99(1999); 2) J.
Biol. Chem.. 274, 11549(1999); 3) Anal. Biochem. 221, 329(1994);
4) J. Am. Chem. Soc. 88, 2015(1966); |
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|
Catelog Number :
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30008-1 |
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Product Name :
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Caspase-3 DEVD-R110
Fluorometric & Colorimetric Assay Kit |
|
Kit Components:
Cell lysis buffer
Assay buffer
Enzyme substrate (Ac-DEVD)2-R110
Enzyme inhibitor Ac-DEVD-CHO
R110
Description:
Caspase-3 is an active cell-death
protease involved in the execution phase of apoptosis,
during which cells undergo morphological changes such as DNA
fragmentation, chromatin condensation, and apoptotic body
formation (1, 2). Caspase-3 DEVD-R110
Fluorometric and Colorimetric Assay Kit provides a simple
assay system for fast and highly sensitive detection of
caspase-3 activity either by fluorescence or absorbance in
mammalian cells. The fluorogenic and chromogenic substrate
(Ac-DEVD)2-R110 contains two DEVD tetrapeptides and is
completely hydrolyzed by the enzyme in two successive steps
(3, 4). Cleavage of the first DEVD peptide
results in the monopeptide Ac-DEVD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second DEVD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Assay Principle below).Although fluorometric
detection of the end products is preferred because of the
superior sensitivity, detection by absorbance is also
possible. In fact, the extinction coefficient of R110 is 10
times higher than that of p-nitroaniline (pNA), a dye
commonly used in chromogenic substrates, making R110- based
substrates significantly more sensitive than pNA-based
substrates, even by colorimetric detection.
The assay kit includes Ac-DEVD-CHO, which is a caspase-3
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-3 activity.
Features:
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
yellow colored and highly green fluorescent rhodamine 110
(R110) product. The long wave- lengths of R110 excitation
and emission minimize cellular autofluorescence.
- Versatile: Compatible with both fluorometric and
colorimetric detection systems.
Related Products:
Staurosporine (#00025 )
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit (#30009-3,
#30009-4 and #30009-5)
Caspase-3 Bioluminescent Assay Kit (# 30010-1, #30010-2 and
#30010-3)
Reference:
1) Cell Death Diff. 6, 99(1999);
2) J. Biol. Chem.. 274, 11549(1999); 3) J. Biol. Chem.
275, 288(2000); 4) Biochemistry, 38, 13906(1999)
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Catelog Number :
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30008-2 |
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Product Name :
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Caspase-3 DEVD-R110
Fluorometric & Colorimetric Assay Kit |
|
Kit Components:
Cell lysis buffer
Assay buffer
Enzyme substrate (Ac-DEVD)2-R110
Enzyme inhibitor Ac-DEVD-CHO
R110
Description:
Caspase-3 is an active cell-death
protease involved in the execution phase of apoptosis,
during which cells undergo morphological changes such as DNA
fragmentation, chromatin condensation, and apoptotic body
formation (1, 2). Caspase-3 DEVD-R110
Fluorometric and Colorimetric Assay Kit provides a simple
assay system for fast and highly sensitive detection of
caspase-3 activity either by fluorescence or absorbance in
mammalian cells. The fluorogenic and chromogenic substrate
(Ac-DEVD)2-R110 contains two DEVD tetrapeptides and is
completely hydrolyzed by the enzyme in two successive steps
(3, 4). Cleavage of the first DEVD peptide
results in the monopeptide Ac-DEVD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second DEVD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Assay Principle below).Although fluorometric
detection of the end products is preferred because of the
superior sensitivity, detection by absorbance is also
possible. In fact, the extinction coefficient of R110 is 10
times higher than that of p-nitroaniline (pNA), a dye
commonly used in chromogenic substrates, making R110- based
substrates significantly more sensitive than pNA-based
substrates, even by colorimetric detection.
The assay kit includes Ac-DEVD-CHO, which is a caspase-3
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-3 activity.
Features:
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
yellow colored and highly green fluorescent rhodamine 110
(R110) product. The long wave- lengths of R110 excitation
and emission minimize cellular autofluorescence.
- Versatile: Compatible with both fluorometric and
colorimetric detection systems.
Related Products:
Staurosporine (#00025 )
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit (#30009-3,
#30009-4 and #30009-5)
Caspase-3 Bioluminescent Assay Kit (# 30010-1, #30010-2 and
#30010-3)
Reference:
1) Cell Death Diff. 6, 99(1999);
2) J. Biol. Chem.. 274, 11549(1999); 3) J. Biol. Chem.
275, 288(2000); 4) Biochemistry, 38, 13906(1999)
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Catelog Number :
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30009-1 |
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Product Name :
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Caspase-3 DEVD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
Cell lysis/assay buffer
Enzyme substrate (Ac-DEVD)2-R110
Enzyme inhibitor Ac-DEVD-CHO
R110
Description:
Caspase-3 DEVD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-3 activity by fluorescence in
enzymatic reaction or mammalian cells. The fluorogenic
substrate (Ac-DEVD)2-R110 contains two DEVD
tetrapeptides and is completely hydrolyzed by the enzyme in
two successive steps 3, 4. Cleavage of the first
DEVD peptide results in the monopeptide Ac-DEVD-R110
intermediate, which has absorption and emission wavelengths
similar to those of R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second DEVD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
The assay kit includes Ac-DEVD-CHO, which is a caspase-3
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-3 activity.
Features:
HTS-compatible: Single-step homogenus assay
specifically designed for HTS-based detection.
Fast: Fast enzyme kinetics.
Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize cellular
autofluorescence.
Related Products:
Staurosporine (#00025)
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
(#30009-1 and #30009-2)
Caspase-3 Bioluminescent Assay Kit (#30010-1, #30010-2 and
#30010-3)
Reference:
1) Cell Death Diff. 6, 99(1999);
2) J. Biol. Chem.. 274, 11549(1999); 3) J. Biol. Chem.
275, 288(2000); 4) Biochemistry, 38, 13906(1999)
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Catelog Number :
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30009-2 |
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Product Name :
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Caspase-3 DEVD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
Cell lysis/assay buffer
Enzyme substrate (Ac-DEVD)2-R110
Enzyme inhibitor Ac-DEVD-CHO
R110
Description:
Caspase-3 DEVD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-3 activity by fluorescence in
enzymatic reaction or mammalian cells. The fluorogenic
substrate (Ac-DEVD)2-R110 contains two DEVD
tetrapeptides and is completely hydrolyzed by the enzyme in
two successive steps 3, 4. Cleavage of the first
DEVD peptide results in the monopeptide Ac-DEVD-R110
intermediate, which has absorption and emission wavelengths
similar to those of R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second DEVD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
The assay kit includes Ac-DEVD-CHO, which is a caspase-3
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-3 activity.
Features:
HTS-compatible: Single-step homogenus assay
specifically designed for HTS-based detection.
Fast: Fast enzyme kinetics.
Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize cellular
autofluorescence.
Related Products:
Staurosporine (#00025)
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
(#30009-1 and #30009-2)
Caspase-3 Bioluminescent Assay Kit (#30010-1, #30010-2 and
#30010-3)
Reference:
1) Cell Death Diff. 6, 99(1999);
2) J. Biol. Chem.. 274, 11549(1999); 3) J. Biol. Chem.
275, 288(2000); 4) Biochemistry, 38, 13906(1999)
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Catelog Number :
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30009-3 |
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Product Name :
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Caspase-3 DEVD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
Cell lysis/assay buffer
Enzyme substrate (Ac-DEVD)2-R110
Enzyme inhibitor Ac-DEVD-CHO
R110
Description:
Caspase-3 DEVD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-3 activity by fluorescence in
enzymatic reaction or mammalian cells. The fluorogenic
substrate (Ac-DEVD)2-R110 contains two DEVD
tetrapeptides and is completely hydrolyzed by the enzyme in
two successive steps 3, 4. Cleavage of the first
DEVD peptide results in the monopeptide Ac-DEVD-R110
intermediate, which has absorption and emission wavelengths
similar to those of R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second DEVD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
The assay kit includes Ac-DEVD-CHO, which is a caspase-3
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-3 activity.
Features:
HTS-compatible: Single-step homogenus assay
specifically designed for HTS-based detection.
Fast: Fast enzyme kinetics.
Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize cellular
autofluorescence.
Related Products:
Staurosporine (#00025)
Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit
(#30009-1 and #30009-2)
Caspase-3 Bioluminescent Assay Kit (#30010-1, #30010-2 and
#30010-3)
Reference:
1) Cell Death Diff. 6, 99(1999);
2) J. Biol. Chem.. 274, 11549(1999); 3) J. Biol. Chem.
275, 288(2000); 4) Biochemistry, 38, 13906(1999)
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Catelog Number :
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30013-3 |
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Product Name :
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Caspase-8
Bioluminescent Assay Kit
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|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-IETD-amino-D-luciferin
- Enzyme inhibitor Ac-IETD-CHO
Description:
Caspase-8 is the most upstream caspase
in the CD95/Fas apoptotic pathway and is activated by the
signaling pathways for CD95/Fas and TNF. The ultra-sensitive
Caspase-8 Bioluminescent Assay Kit is based on the same
principle as the Caspase-3 Bioluminescent Assay Kit is based
on (See description for #30010-1 and #30010-2). The kit
employs the substrate Ac-IETD-amino-D-luciferin in
combination with firefly luciferase to detect caspase-8. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-8 activity in mammalian
cells.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
- Staurosporine (#00025)
- Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay
Kit (#30012-1 and #30012-2)
- Caspase-8 DEVD-R110 Fluorometric HTS Assay Kit
(#30012-3, #30012-4 and #30012-5)
Reference:
1) Biochemistry, 38, 13906(1999). |
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Catelog Number :
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30013-2 |
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Product Name :
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|
|
Caspase-8
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-IETD-amino-D-luciferin
- Enzyme inhibitor Ac-IETD-CHO
Description:
Caspase-8 is the most upstream caspase
in the CD95/Fas apoptotic pathway and is activated by the
signaling pathways for CD95/Fas and TNF. The ultra-sensitive
Caspase-8 Bioluminescent Assay Kit is based on the same
principle as the Caspase-3 Bioluminescent Assay Kit is based
on (See description for #30010-1 and #30010-2). The kit
employs the substrate Ac-IETD-amino-D-luciferin in
combination with firefly luciferase to detect caspase-8. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-8 activity in mammalian
cells.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
- Staurosporine (#00025)
- Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay
Kit (#30012-1 and #30012-2)
- Caspase-8 DEVD-R110 Fluorometric HTS Assay Kit
(#30012-3, #30012-4 and #30012-5)
Reference:
1) Biochemistry, 38, 13906(1999). |
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Catelog Number :
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30013-1 |
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Product Name :
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|
Caspase-8
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-IETD-amino-D-luciferin
- Enzyme inhibitor Ac-IETD-CHO
Description:
Caspase-8 is the most upstream caspase
in the CD95/Fas apoptotic pathway and is activated by the
signaling pathways for CD95/Fas and TNF. The ultra-sensitive
Caspase-8 Bioluminescent Assay Kit is based on the same
principle as the Caspase-3 Bioluminescent Assay Kit is based
on (See description for #30010-1 and #30010-2). The kit
employs the substrate Ac-IETD-amino-D-luciferin in
combination with firefly luciferase to detect caspase-8. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-8 activity in mammalian
cells.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
- Staurosporine (#00025)
- Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay
Kit (#30012-1 and #30012-2)
- Caspase-8 DEVD-R110 Fluorometric HTS Assay Kit
(#30012-3, #30012-4 and #30012-5)
Reference:
1) Biochemistry, 38, 13906(1999). |
|
|
Catelog Number :
|
|
|
30011-1 |
|
|
Product Name :
|
|
|
Caspase-8 IETD-R110
Fluorometric and Colorimetric Assay Kit |
|
Kit Components:
Cell lysis buffer
Assay buffer
Enzyme substrate (Ac-IETD)2-R110
Enzyme inhibitor Ac-IETD-CHO
R110
Description:
Caspase-8 is the most upstream caspase
in the CD95/Fas apoptotic pathway and is activated by the
signaling pathways for CD95/Fas and TNF. Caspase-8
Fluorometric and Colorimetric Assay Kit provides a simple
assay system for fast and sensitive detection of caspase-8
activity either by fluorescence or absorbance in mammalian
cells. The kit includes the fluorogenic substrate (Ac-IETD)2-R110,
the enzyme inhibitor Ac-IETD-CHO as a negative control, R110
as a standard and optimized lysis and assay buffers. The
substrate (Ac-IETD)2-R110 contains two IETD
tetrapeptides and is completely hydrolyzed by the enzyme in
a two-step process. Cleavage of the first IETD results in
the monopeptide Ac- IETD-R110 intermediate, which has
absorption and emission wavelengths similar to those of R110
(lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second IETD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).Although fluorometric
detection of the end products is preferred because of the
superior sensitivity, detection by absorbance is also
possible. In fact, the extinction coefficient of R110 is 10
times higher than that of p-nitroaniline (pNA), a dye
commonly used in chromogenic substrates, making R110- based
substrates significantly more sensitive than pNA-based
substrates, even by colorimetric detection.
Features:
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
yellow colored and highly green fluorescent rhodamine 110
(R110) product. The long wave lengths of R110 excitation and
emission minimize cellular autofluorescence.
- Versatile: Compatible with both fluorometric and
colorimetric detection systems.
Related Products:
Staurosporine (#00025)
Caspase-8 DEVD-R110 Fluorometric HTS Assay Kit (#30012-3,
#30012-4 and #30012-5)
Caspase-8 Bioluminescent Assay Kit (#30013-1, #30013-2 and
#30013-3)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30011-2 |
|
|
Product Name :
|
|
|
Caspase-8 IETD-R110
Fluorometric and Colorimetric Assay Kit |
|
Kit Components:
Cell lysis buffer
Assay buffer
Enzyme substrate (Ac-IETD)2-R110
Enzyme inhibitor Ac-IETD-CHO
R110
Description:
Caspase-8 is the most upstream caspase
in the CD95/Fas apoptotic pathway and is activated by the
signaling pathways for CD95/Fas and TNF. Caspase-8
Fluorometric and Colorimetric Assay Kit provides a simple
assay system for fast and sensitive detection of caspase-8
activity either by fluorescence or absorbance in mammalian
cells. The kit includes the fluorogenic substrate (Ac-IETD)2-R110,
the enzyme inhibitor Ac-IETD-CHO as a negative control, R110
as a standard and optimized lysis and assay buffers. The
substrate (Ac-IETD)2-R110 contains two IETD
tetrapeptides and is completely hydrolyzed by the enzyme in
a two-step process. Cleavage of the first IETD results in
the monopeptide Ac- IETD-R110 intermediate, which has
absorption and emission wavelengths similar to those of R110
(lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second IETD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).Although fluorometric
detection of the end products is preferred because of the
superior sensitivity, detection by absorbance is also
possible. In fact, the extinction coefficient of R110 is 10
times higher than that of p-nitroaniline (pNA), a dye
commonly used in chromogenic substrates, making R110- based
substrates significantly more sensitive than pNA-based
substrates, even by colorimetric detection.
Features:
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
yellow colored and highly green fluorescent rhodamine 110
(R110) product. The long wave lengths of R110 excitation and
emission minimize cellular autofluorescence.
- Versatile: Compatible with both fluorometric and
colorimetric detection systems.
Related Products:
Staurosporine (#00025)
Caspase-8 DEVD-R110 Fluorometric HTS Assay Kit (#30012-3,
#30012-4 and #30012-5)
Caspase-8 Bioluminescent Assay Kit (#30013-1, #30013-2 and
#30013-3)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30012-3 |
|
|
Product Name :
|
|
|
Caspase-8 IETD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
Cell lysis/assay buffer
Enzyme substrate (Ac-IETD)2-R110
Enzyme inhibitor Ac-IETD-CHO
R110
Description:
Caspase-8 IETD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-8 activity by fluorescence in
enzymatic reaction or mammalian cells. The fluorogenic
substrate (Ac-IETD)2-R110 contains two IETD
tetrapeptides and is completely hydrolyzed by the enzyme in
two successive steps. Cleavage of the first IETD peptide
results in the monopeptide Ac-IETD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second IETD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).The assay kit
includes Ac-IETD-CHO, which is a caspase-8 inhibitor and can
be used as a negative control. Also, R110 is provided in the
kit for generating a standard curve, which can be used for
quantifying caspase-8 activity.
Features:
- HTS-compatible: Single-step homogenous assay
specifically designed for HTS-based detection.
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize
cellular autofluorescence.
Related Products:
Staurosporine (#00025)
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit
(#30012-1 and #30012-2)
Caspase-8 Bioluminescent Assay Kit (#30013-1, #30013-2 and
#30013-3)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30012-1 |
|
|
Product Name :
|
|
|
Caspase-8 IETD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
Cell lysis/assay buffer
Enzyme substrate (Ac-IETD)2-R110
Enzyme inhibitor Ac-IETD-CHO
R110
Description:
Caspase-8 IETD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-8 activity by fluorescence in
enzymatic reaction or mammalian cells. The fluorogenic
substrate (Ac-IETD)2-R110 contains two IETD
tetrapeptides and is completely hydrolyzed by the enzyme in
two successive steps. Cleavage of the first IETD peptide
results in the monopeptide Ac-IETD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (lex\lem
= 496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second IETD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).The assay kit
includes Ac-IETD-CHO, which is a caspase-8 inhibitor and can
be used as a negative control. Also, R110 is provided in the
kit for generating a standard curve, which can be used for
quantifying caspase-8 activity.
Features:
- HTS-compatible: Single-step homogenous assay
specifically designed for HTS-based detection.
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize
cellular autofluorescence.
Related Products:
Staurosporine (#00025)
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit
(#30012-1 and #30012-2)
Caspase-8 Bioluminescent Assay Kit (#30013-1, #30013-2 and
#30013-3)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30012-2 |
|
|
Product Name :
|
|
|
Caspase-8 IETD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
Cell lysis/assay buffer
Enzyme substrate (Ac-IETD)2-R110
Enzyme inhibitor Ac-IETD-CHO
R110
Description:
Caspase-8 IETD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-8 activity by fluorescence in
enzymatic reaction or mammalian cells. The fluorogenic
substrate (Ac-IETD)2-R110 contains two IETD
tetrapeptides and is completely hydrolyzed by the enzyme in
two successive steps. Cleavage of the first IETD peptide
results in the monopeptide Ac-IETD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (lex\lem=
496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second IETD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).The assay kit
includes Ac-IETD-CHO, which is a caspase-8 inhibitor and can
be used as a negative control. Also, R110 is provided in the
kit for generating a standard curve, which can be used for
quantifying caspase-8 activity.
Features:
- HTS-compatible: Single-step homogenous assay
specifically designed for HTS-based detection.
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize
cellular autofluorescence.
Related Products:
Staurosporine (#00025)
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit
(#30012-1 and #30012-2)
Caspase-8 Bioluminescent Assay Kit (#30013-1, #30013-2 and
#30013-3)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30016-3 |
|
|
Product Name :
|
|
|
Caspase-9
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-LEHD-amino-D-luciferin
- Enzyme inhibitor Ac-LEHD-CHO
Description:
Caspase-9 is an upstream caspase for
mitochondria- specific apoptosis pathway and is activated
via the mitochondrial release of cytochrome c to the
cytosol. The ultra-sensitive Caspase-9 Bioluminescent Assay
Kit is based on the same principle as the Caspase-3
Bioluminescent Assay Kit is based on (See description for
#30010-1 and #30010-2). The kit employs the substrate
Ac-LEHD-amino-D-luciferin in combination with Firefly
luciferase to detect the peptidase. The kit provides a
homogenous assay system for fast and highly sensitive
detection of caspase-9 activity in mammalian cells.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
Staurosporine (#00025)
Caspase-9 LEHD-R110 Fluorometric and Colorimetric Assay Kit
(#30015-1 and #30015-2)
Caspase-9 LEHD-R110 Fluorometric HTS Assay Kit (#30016- 3
and #30016-4)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30016-1 |
|
|
Product Name :
|
|
|
Caspase-9
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-LEHD-amino-D-luciferin
- Enzyme inhibitor Ac-LEHD-CHO
Description:
Caspase-9 is an upstream caspase for
mitochondria- specific apoptosis pathway and is activated
via the mitochondrial release of cytochrome c to the
cytosol. The ultra-sensitive Caspase-9 Bioluminescent Assay
Kit is based on the same principle as the Caspase-3
Bioluminescent Assay Kit is based on (See description for
#30010-1 and #30010-2). The kit employs the substrate
Ac-LEHD-amino-D-luciferin in combination with Firefly
luciferase to detect the peptidase. The kit provides a
homogenous assay system for fast and highly sensitive
detection of caspase-9 activity in mammalian cells.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
Staurosporine (#00025)
Caspase-9 LEHD-R110 Fluorometric and Colorimetric Assay Kit
(#30015-1 and #30015-2)
Caspase-9 LEHD-R110 Fluorometric HTS Assay Kit (#30016- 3
and #30016-4)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30016-2 |
|
|
Product Name :
|
|
|
Caspase-9
Bioluminescent Assay Kit
|
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate Ac-LEHD-amino-D-luciferin
- Enzyme inhibitor Ac-LEHD-CHO
Description:
Caspase-9 is an upstream caspase for
mitochondria- specific apoptosis pathway and is activated
via the mitochondrial release of cytochrome c to the
cytosol. The ultra-sensitive Caspase-9 Bioluminescent Assay
Kit is based on the same principle as the Caspase-3
Bioluminescent Assay Kit is based on (See description for
#30010-1 and #30010-2). The kit employs the substrate
Ac-LEHD-amino-D-luciferin in combination with Firefly
luciferase to detect the peptidase. The kit provides a
homogenous assay system for fast and highly sensitive
detection of caspase-9 activity in mammalian cells.
Features:
- Ultra-sensitive: Detectable with fewer than 100
cells with excellent signal-to-noise ratio.
- Simple: Single-step homogenous assay.
- HTS-compatible: The extended-glow signal is
compatible with HTS format.
Related Products:
Staurosporine (#00025)
Caspase-9 LEHD-R110 Fluorometric and Colorimetric Assay Kit
(#30015-1 and #30015-2)
Caspase-9 LEHD-R110 Fluorometric HTS Assay Kit (#30016- 3
and #30016-4)
Reference:
1) Biochemistry, 38, 13906(1999).
|
|
|
Catelog Number :
|
|
|
30014-1
|
|
|
Product Name :
|
|
|
Caspase-9 LEHD-R110
Fluorometric and Colorimetric Assay Kit |
|
Kit Components:
- Cell lysis buffer
- Assay buffer
- Enzyme substrate (Ac-LEHD)2-R110
- Enzyme inhibitor Ac-LEHD-CHO
- R110
Description:
Caspase-9 is an upstream caspase for
mitochondria- specific apoptosis pathway and is activated
via the mitochondrial release of cytochrome c to the
cytosol. Caspase- 9 Fluorometric and Colorimetric Assay Kit
provides a simple assay system for fast and sensitive
detection of caspase-9 activity either by fluorescence or
absorbance in mammalian cells. The kit includes the
fluorogenic substrate (Ac-LEHD))2- R110, the
enzyme inhibitor Ac-LEHD-CHO as a negative control, R110 as
a standard and optimized lysis and assay buffers. The
substrate (Ac-LEHD))2-R110 contains two LEHD
tetrapeptides and is completely hydrolyzed by the enzyme in
a two-step process. Cleavage of the first LEHD peptide
results in the monopeptide Ac-LEHD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (labs/lem
= 496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second LEHD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
Although fluorometric detection of the end products is
preferred because of the superior sensitivity, detection by
absorbance is also possible. In fact, the extinction
coefficient of R110 is 10 times higher than that of
p-nitroaniline (pNA), a dye commonly used in chromogenic
substrates, making R110- based substrates significantly more
sensitive than pNA-based substrates, even by colorimetric
detection.
Features:
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
yellow colored and highly green fluorescent rhodamine 110
(R110) product. The long wave- lengths of R110 excitation
and emission minimize cellular autofluorescence.
- Versatile: Compatible with both fluorometric and
colorimetric detection systems.
Related Products:
Staurosporine (#00025) Caspase-9 LEHD-R110
Fluorometric HTS Assay Kit (#30015-3, #30015-4 and #30015-5)
Caspase-9 Bioluminescent Assay Kit (#30016-1, #30016-2 and
#30016-3)
Reference:
1) Biochemistry, 38, 13906(1999). |
|
|
Catelog Number :
|
|
|
30014-2 |
|
|
Product Name :
|
|
|
Caspase-9 LEHD-R110
Fluorometric and Colorimetric Assay Kit |
|
Kit Components:
- Cell lysis buffer
- Assay buffer
- Enzyme substrate (Ac-LEHD)2-R110
- Enzyme inhibitor Ac-LEHD-CHO
- R110
Description:
Caspase-9 is an upstream caspase for
mitochondria- specific apoptosis pathway and is activated
via the mitochondrial release of cytochrome c to the
cytosol. Caspase- 9 Fluorometric and Colorimetric Assay Kit
provides a simple assay system for fast and sensitive
detection of caspase-9 activity either by fluorescence or
absorbance in mammalian cells. The kit includes the
fluorogenic substrate (Ac-LEHD))2- R110, the
enzyme inhibitor Ac-LEHD-CHO as a negative control, R110 as
a standard and optimized lysis and assay buffers. The
substrate (Ac-LEHD))2-R110 contains two LEHD
tetrapeptides and is completely hydrolyzed by the enzyme in
a two-step process. Cleavage of the first LEHD peptide
results in the monopeptide Ac-LEHD-R110 intermediate, which
has absorption and emission wavelengths similar to those of
R110 (labs/lem
= 496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second LEHD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
Although fluorometric detection of the end products is
preferred because of the superior sensitivity, detection by
absorbance is also possible. In fact, the extinction
coefficient of R110 is 10 times higher than that of
p-nitroaniline (pNA), a dye commonly used in chromogenic
substrates, making R110- based substrates significantly more
sensitive than pNA-based substrates, even by colorimetric
detection.
Features:
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
yellow colored and highly green fluorescent rhodamine 110
(R110) product. The long wave- lengths of R110 excitation
and emission minimize cellular autofluorescence.
- Versatile: Compatible with both fluorometric and
colorimetric detection systems.
Reference:
1) Biochemistry, 38, 13906(1999). |
|
|
Catelog Number :
|
|
|
30015-1 |
|
|
Product Name :
|
|
|
Caspase-9 LEHD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate (Ac-LEHD)2-R110
- Enzyme inhibitor Ac-LEHD-CHO
- R110
Description:
Caspase-9 LEHD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-9 activity by fluorescence in
mammalian cells. The fluorogenic substrate (Ac-LEHD)2-R110
contains two LEHD tetrapeptides and is completely hydrolyzed
by the enzyme in two successive steps. Cleavage of the first
LEHD peptide results in the monopeptide Ac-LEHDR110
intermediate, which has absorption and emission wavelengths
similar to those of R110 (labs/lem
= 496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second LEHD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
The assay kit includes Ac-LEHD-CHO, which is a caspase-9
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-9 activity.
Features:
HTS-compatible: Single-step homogenous assay
specifically designed for HTS-based detection.
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize
cellular autofluorescence.
Related Products:
- Staurosporine (#00025)
- Caspase-9 LEHD-R110 Fluorometric and Colorimetric Assay
Kit (#30015-1 and #30015-2)
- Caspase-9 Bioluminescent Assay Kit (#30016-1, #30016-2
and #30016-3)
Reference:
1) Biochemistry, 38, 13906(1999). |
|
|
Catelog Number :
|
|
|
30015-2 |
|
|
Product Name :
|
|
|
Caspase-9 LEHD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate (Ac-LEHD)2-R110
- Enzyme inhibitor Ac-LEHD-CHO
- R110
Description:
Caspase-9 LEHD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-9 activity by fluorescence in
mammalian cells. The fluorogenic substrate (Ac-LEHD)2-R110
contains two LEHD tetrapeptides and is completely hydrolyzed
by the enzyme in two successive steps. Cleavage of the first
LEHD peptide results in the monopeptide Ac-LEHDR110
intermediate, which has absorption and emission wavelengths
similar to those of R110 (labs/lem
= 496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second LEHD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
The assay kit includes Ac-LEHD-CHO, which is a caspase-9
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-9 activity.
Features:
HTS-compatible: Single-step homogenous assay
specifically designed for HTS-based detection.
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize
cellular autofluorescence.
Related Products:
- Staurosporine (#00025)
- Caspase-9 LEHD-R110 Fluorometric and Colorimetric Assay
Kit (#30015-1 and #30015-2)
- Caspase-9 Bioluminescent Assay Kit (#30016-1, #30016-2
and #30016-3)
Reference:
1) Biochemistry, 38, 13906(1999). |
|
|
Catelog Number :
|
|
|
30015-3 |
|
|
Product Name :
|
|
|
Caspase-9 LEHD-R110
Fluorometric HTS Assay Kit |
|
Kit Components:
- Cell lysis/assay buffer
- Enzyme substrate (Ac-LEHD)2-R110
- Enzyme inhibitor Ac-LEHD-CHO
- R110
Description:
Caspase-9 LEHD-R110 Fluorometric HTS
Assay Kit is specifically designed for HTS-based assays. The
kit provides a homogenous assay system for fast and highly
sensitive detection of caspase-9 activity by fluorescence in
mammalian cells. The fluorogenic substrate (Ac-LEHD)2-R110
contains two LEHD tetrapeptides and is completely hydrolyzed
by the enzyme in two successive steps. Cleavage of the first
LEHD peptide results in the monopeptide Ac-LEHDR110
intermediate, which has absorption and emission wavelengths
similar to those of R110 (labs/lem
= 496/520 nm) but has only about 10% of the fluorescence of
the latter. Hydrolysis of the second LEHD peptide releases
the dye R110, leading to a substantial fluorescence increase
(See Figure B3 for assay principle).
The assay kit includes Ac-LEHD-CHO, which is a caspase-9
inhibitor and can be used as a negative control. Also, R110
is provided in the kit for generating a standard curve,
which can be used for quantifying caspase-9 activity.
Features:
HTS-compatible: Single-step homogenous assay
specifically designed for HTS-based detection.
- Fast: Fast enzyme kinetics.
- Sensitive: The enzymatic reaction forms intensely
green fluorescent rhodamine 110 (R110) product. The long
wavelengths of R110 excitation and emission minimize
cellular autofluorescence.
Related Products:
- Staurosporine (#00025)
- Caspase-9 LEHD-R110 Fluorometric and Colorimetric Assay
Kit (#30015-1 and #30015-2)
- Caspase-9 Bioluminescent Assay Kit (#30016-1, #30016-2
and #30016-3)
Reference:
1) Biochemistry, 38, 13906(1999). |
|
|
Catelog Number :
|
|
|
30001 |
|
|
Product Name :
|
|
|
JC-1 mitochondrial
membrane potential detection kit |
|
Kit Components:
1. 500 uL 100X JC-1
2. 10 mL 10X buffer
Description:
The loss of mitochondrial membrane
potential is a hallmark for apoptosis. It is an early event
preceding phosphatidylserine externalization and coinciding
with caspase activation 1-2. JC-1 Mitochondrial
Membrane Potential Detection Kit is used to measure
mitochondrial membrane potential changes in cells. In
non-apoptotic cells, JC-1 accumulates as aggregates in the
mitochondrial membranes, resulting in red fluorescence (590
nm). The brightness of red fluorescence is proportional to
the potential and varies among different cell types.
However, in apoptotic and necrotic cells, which have
diminished mitochondrial membrane potential,JC-1 exists in
the green fluorescent (529 nm) monomeric form 3-5.
This kit provides a step-by-step protocol and ready-to-use
reagents for performing >100 assays for use on flow
cytometers, fluorescence microscopes and fluorometric plate
readers.
Features:
- Fast and simple: 15 minutes of incubation with
the dye, followed by washing and measurement.
- Cell membrane-permeable: Direct detection of
apoptosis and mitochondrial membrane potential on live
cells.
- Versatile: Detection compatible with flow
cytometers, fluorescence microscopes and fluorometric plate
readers.
Related Products:
Staurosporine
Also see our extensive list of fluorogenic and bioluminogenic
caspase substrates.
Reference:
1) Exp Hematol. 31, 815(2003); 2) Br J
Haematol. 108, 574(2000); 3) Cytometry 29, 97(1997); 4) FEBS
Lett 411, 77(1997); 5) J Neurochem 70, 66(1998). |
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Catelog Number :
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30019 |
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Product Name :
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MCB Glutathione
Detection Kit |
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Kit Components:
- Cell lysis buffer
- Monochlorobimane (MCB)
- GST postive control
Description:
Diminished cellular glutathione (GSH)
level occurs at the early stage of mitochondrion-associated
apoptosis pathway due to GSH efflux. GSH depletion further
leads to cytochrome c release and caspase 3 induction (1,2)
MCB Glutathione Detection Kit utilizes a thiol-reactive dye
monochlorobimane (MCB), which is essentially monfluorescent
until it reacts with a thiol to form a blue fluorescent
product (labs/lem
= 380/461 nm). By incubating cellular lysate with MCB, the
intensity of the fluorescent signal generated from the assay
reflects the amount of GSH present in the cells.
Fluorescence can easily be detected using a fluorometer or
96- well fluorometric plate reader.
Features:
- Simple: Compatible with a standard or
fluorescence plate reader.
- Fast: Around 1 hour assay time.
Related Products:
- Apoptosis & Necrosis Quantification Kit
- Apoptotic, Necrotic & Healthy Cells Quantification Kit
- JC-1 Mitochondrial Membrane Potential Detection Kit
- Staurosporine
Also see our extensive list of fluorogenic and luminogenic
caspase substrates.Reference:
1) FASEB J. 12(6), 479(1998); 2)
Biochem. Soc. Trans. 28, 56(2000). |
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