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| |
Amine-reactive Dyes and Related Reagents
Product Specification
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Catelog Number :
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90000 |
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Product Name :
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|
5-
(and-6)-Carboxyrhodamine 110, hydrochloride (5(6)-CR 110) |
Description:
For some applications, 5-(and-6)-carboxyrhodamine is a
superior alternative to the widely used green fluorescent
dye 5-(and-6)-carboxyfluorescein. The dye is insensitive to
pH between 4 and 9 and is much more photostable than
fluorescein. See spectra and photostability data under # 90081.
See Figure 7.5 for spectra.
- lex\lem(MeOH)
= 502/524 nm.
- Orange red solid soluble in H2O (pH>6),
DMF or DMSO.
- Store at 4°C and protect from light.
- C21H15ClN2O5.
- Mwt: 410.81
|
Chemical Structure of 90000
Reference: |
|
Catelog Number :
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90041 |
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Product Name :
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5(6)-CFDA, SE
(5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester) |
Description:
5(6)-CFDA, SE is a useful fluorescent tracer that can
passivley diffuse into cells and covalently label
intracellular proteins, resulting in long-term cell
labeling. The reagent itself is colorless and nonfluorescent
but becomes brightly green fluorescent once it is hydrolyzed
by intracellular esterases. The labeling has been shown to
be stable to formaldehyde or glutaldehyde fixation
condition.
- lex\lem(of
hydrolysis product at pH 7) = 495/519 nm
- White solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C29H19NO11
- Mwt: 557 ! [150347-59-4]
|
Reference:
1) J. Cell Biol. 101, 610(1985); 2) J. Cell Biol.
103, 2649(1986); 3) Cell Transplantation 3, 397(1994). |
|
Catelog Number :
|
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|
90044 |
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|
Product Name :
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|
5(6)-SFX
(6-(fluorescein-5-(and-6)-carboxamido)hexanoic acid,succinimidyl
ester) |
Description:
5(6)-SFX has a 6-atom aminohexanoyl spacer group that
separates the fluorescein dye and the amine-reactive group.
See Figure 7.4 for spectra.
- lex\lem(pH9)
= 494/521nm
- Yellow solid soluble in DMF, DMSO or pH>6.5 buffers
- Store at -20°C and protect from light
- C31H26N2O10
- Mwt:586.55
|
Reference: |
|
Catelog Number :
|
|
|
90040 |
|
|
Product Name :
|
|
|
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate,
succinimidyl ester |
Description:
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate,
succinimidyl ester is a useful fluorescent tracer that can
passively diffuse into cells and covalently label
intracellular proteins, resulting in long-term cell
labeling. The reagent itself is colorless and nonfluorescent
but becomes brightly green fluorescent once it is hydrolyzed
by intracellular esterases. The fluorescence of the dye is
relatively insensitive to pH change and the labeling has
been shown to be stable to formaldehyde or glutaldehyde
fixation condition.
- lex\lem(of
hydrolysis product at pH 7) = 504/529 nm
- White solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C29H17Cl2NO11
- Mwt: 626
|
Reference:
Development 116, 1087(1992). |
|
Catelog Number :
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|
90024 |
|
|
Product Name :
|
|
|
5-(and-6)-Carboxyfluorescein, succinimidyl ester (5(6)-FAM,SE)
(mixed isomers) |
Description:
5(6)-FAM SE is an amine-reactive green fluorescent dye
widely used for labeling proteins or other biomolecules that
contain a primary or secondary aliphatic amine. The coupling
reaction is usually carried out at pH 8-9.5. The amide
linkage from the coupling reaction is much more stable than
the thiourea linkage formed from the coupling of an amine
and an isothiocyanate. See Figure 7.4 for spectra.
- lex\lem
= 495/519 nm.
- Yellow solid soluble in DMSO or DMF.
- Store at -20°C and protect from light.
- C25H15NO9.
- Mwt: 473.4.
- [117548-22-8]
|
Chemical Structure of 90024
Reference: |
|
Catelog Number :
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|
90001 |
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Product Name :
|
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5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed
isomers (5(6)-CR 110, SE or Carboxyrhodamine 110-NHS) |
Description:
5-(and-6)-Carboxyrhodamine 110, succinimidyl ester is a
superior green fluorescent dye that readily reacts with a
primary or secondary amine. For many applications, the dye
is preferred over 5-(and-6)-carboxyfluorescein succinimidyl
ester (5(6)-FAM) or FITC because of its exceptional
photostability and fluorescence insensitivity to pH (4-9).
See spectra and photostability data under #90081. Also see # 90030,
# 90031, # 90081 and
# 90084. See Figure 7.5 for spectra.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C25H17N3O7
- Mwt:471.42
|
Chemical Structure of 90001
Reference: |
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Catelog Number :
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90002 |
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Product Name :
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5-(and-6)-Carboxyrhodamine 110-X, hydrochloride (5(6)-CR 110-X) |
Description:
5-(and-6)-Carboxyrhodamine 110-X is similar to
5-(and-6)-carboxyrhodamine 110 (# 90000).
The X linker molecule is useful to reduce the interaction
between the dye and the biomolecule to be labeled. See
Figure 7.5 for spectra and Figure 7.6 for photostability
comparison with other dyes.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in water, DMF or DMSO
- Store at 4°C and protect from light
- C28H30ClN3O6.
- Mwt:524.
|
Chemical Structure of 90002
Reference: |
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Catelog Number :
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90003 |
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Product Name :
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5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester (5(6)-CR
110-X, SE) |
Description:
5-(and-6)-Carboxyrhodamine 110-X, succinimidyl ester readily
couples to a primary or secondary amine. The dye is a
superior alternative to 5(6)-SFX because of its exceptional
phototostability and fluorescence insensitivity to pH (4-9).
See Figure 7.5 for spectra and Figure 7.6 for photostability
comparison with other dyes.
- lex\lem
(MeOH) = 502/524 nm.
- Orange red solid soluble in DMSO or DMF.
- Store at -20°C and protect from light
- C31H28N4O8
- Mwt: 584.58
|
Reference: |
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Catelog Number :
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90022 |
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Product Name :
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5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed
isomers) (5(6)-TAMRA SE) |
Description:
Carboxytetramethylrhodamine is one of the most commonly used
red fluorescent dye. The succinimidyl ester reacts readily
with primary or secondary amines under mild conditions. See
Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- e = 95,000
- Red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C29H25N3O7.
- Mwt: 527.5
- [150408-83-6]
|
Chemical Structure of 90022
Reference: |
|
Catelog Number :
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90027 |
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Product Name :
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5-(and-6)-Carboxytetramethylrhodamine, mixed isomers
(5(6)-TAMRA) |
Description:
Mixed isomers of carboxytetramethylrhodamine free acid. The
dye can be coupled to primary or secondary amines via
standard peptide chemistry. See Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solid soluble in DMSO, DMF, MeOH, or pH
>6.5 buffer
- Store at -20°C and protect from light
- C25H23ClN2O5.
- Mwt: 466.92
|
Chemical Structure of 90027
Reference: |
|
Catelog Number :
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90005 |
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Product Name :
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5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester (mixed
isomers) (5(6)-ROX, SE) |
Description:
5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester is an
amine-reactive long wavelength rhodamine dye. See Figure 7.9
for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C37H33N3O7.
- Mwt: 631.7
- [114616-32-9]
|
Chemical Structure of 90005
Reference: |
|
Catelog Number :
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|
90004 |
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Product Name :
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5-(and-6)-Carboxy-X-rhodamine, triethylammonium salt (mixed
isomers) (5(6)-ROX) |
Description:
5-(and-6)-Carboxy-X-rhodamine is a long wavelength rhodamine
dye. See Figure 7.9 for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO, DMF or MeOH
- Store at -20°C and protect from light
- C39H45N3O5.
- Mwt: 635.6
|
Chemical Structure of 90004
Reference: |
|
Catelog Number :
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51019 |
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Product Name :
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5-Carboxyfluorescein (5-FAM, single isomer) |
Description:
Free acid form of 5-carboxyfluorescein single isomer. See
Figure 7.4 for spectra.
- lex\lem(pH
9) = 492/514nm
- Yellow orange solid soluble in pH>6 water
- Store at 4°C and protect from light, especially in
solution
- C21H12O7
- Mwt: 376.3
|
Chemical Structure of 51019
Reference: |
|
Catelog Number :
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90029 |
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Product Name :
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|
5-Carboxyfluorescein, succinimidyl ester (5-FAM, SE) (single
isomer) |
Description:
Amine-reactive form of 5-carboxyfluorescein single isomer.
See Figure 7.4 for spectra.
- lex\lem(pH
9) = 495/519 nm
- Yellow orange solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C25H15NO9.
- Mwt: 473.4
- [92557-80-7]
|
Chemical Structure of 90029
Reference: |
|
Catelog Number :
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90030 |
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Product Name :
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5-Carboxyrhodamine
110, succinimidyl ester, single isomer (5-CR110, SE) |
Description:
Amine-reactive form of single isomer carboxyrhodamine 110,
useful in DNA sequencing. For many single-dye-labeling
applications, the dye is preferred over other green
fluorescent dyes including fluorescein and Alexa 488 because
of its exceptional photostability and fluorescence
insensitivity to pH (4-9). See spectra and photostability
data under #90081. See Figure 7.5 for spectra.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C25H17N3O7.
- Mwt: 471.42
|
Chemical Structure of 90030
Reference: |
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Catelog Number :
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90086 |
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Product Name :
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5-Carboxyrhodamine
110-PEO12-propionate, succinimidyl ester, single
isomer (5-CR110-PEO12, SE) |
Description:
5-CR110-PEO 12 SE has a long, water-soluble PEG
spacer between the green fluorescent rhodamine dye and the
amine-reactive SE group. See Figure 7.5 for spectra and
Figure 7.6 for photostability comparison with other dyes.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C52H70N4O20
- Mwt: 1071.13
|
Chemical Structure of 90086
Reference: |
|
Catelog Number :
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90081 |
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Product Name :
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5-Carboxyrhodamine
110-PEO4-propionate, succinimidyl ester, single
isomer (5-CR110-PEO4, SE) |
Description:
NH 5-CR110-PEO 4 SE is an excellent green
fluorescent rhodamine dye for labeling amines. The dye has
better solubility than the parent dye 5-CR110 SE and its
long PEG spacer should reduce any steric effect of the dye
on the molecule to be labeled. Carboxyrhodamine 110 and its
derivatives are, in general, significantly more photostable
than any other known green fluorescent dyes including Alexa
488. In addition, their spectra are unaffected by pH change
from 4 to 9. Unlike Alexa 488, which is readily degraded
under alkaline condition, CR110 and its derivatives are
highly stable under both acidic and basic conditions. As a
result, CR110 and its derivatives are superior alternatives
to other green fluorescent dyes for applications where only
a single dye is required for the labeling. See Figure 7.5
for spectra and Figure 7.6 for photostability comparison
with other dyes.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C36H38N4O12
- Mwt: 718.71
|
Chemical Structure of 90081
Reference: |
|
Catelog Number :
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90084 |
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Product Name :
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5-Carboxyrhodamine
110-PEO8-propionate, succinimidyl ester, single
isomer (5-CR110-PEO8, SE) |
Description:
5-CR110-PEO 8 SE is similar to 5-CR110-PEO 4
SE except that it has an even longer PEG spacer. See Figure
7.5 for spectra and Figure 7.6 for photostability comparison
with other dyes.
- lex\lem
(MeOH) = 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C44H54N4O16
- Mwt: 894.92
|
Chemical Structure of 90084
Reference: |
|
Catelog Number :
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90014 |
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Product Name :
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5-Carboxyrhodamine
110-X, succinimidyl ester (5-CR110-X,SE) |
Description:
Amine-reactive form of 5-carboxyrhodamine 110 with a
6-carbon spacer. See Figure 7.5 for spectra and Figure 7.6
for photostability comparison with other dyes.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C31H28N4O8
- Mwt: 584.58
|
Reference: |
|
Catelog Number :
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|
90012 |
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|
Product Name :
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5-Carboxyrhodamine
6G, hydrochloride, single isomer (5-CR6G) |
Description:
Free acid form of 5-carboxyrhodamine 6G single isomer. See
Figure 7.7 for spectra.
- lex\lem(MeOH)
= 520/546 nm
- Orange red solid soluble in water (pH 7), DMF or
DMSO
- Store at -20°C and protect from light
- C27H27ClN2O5.
- Mwt: 494.97
|
Chemical Structure of 90012
Reference: |
|
Catelog Number :
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90032 |
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Product Name :
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5-Carboxyrhodamine
6G, succinimidyl ester (single isomer)(5-CR6G SE) |
Description:
Amine-reactive form of 5-carboxyrhodamine 6G single isomer.
See Figure 7.7 for spectra.
- lex\lem(MeOH)
= 520/546 nm
- Orange red solid moderately soluble in DMSO
- Store at -20°C and protect from light
- C31H29ClN3O7
- Mwt: 555.6
|
Chemical Structure of 90032
Reference: |
|
Catelog Number :
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90034 |
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Product Name :
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5-Carboxytetramethylrhodamine succinimidyl ester (single isomer)
( 5-TAMRA SE) |
Description:
Amine-reactive form of 5-carboxytetramethylrhodamine single
isomer. See Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C29H25N3O7.
- Mwt: 527.5
- [150810-68-7]
|
Chemical Structure of 90034
Reference: |
|
Catelog Number :
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|
90009 |
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Product Name :
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5-Carboxytetramethylrhodamine, single isomer (5-TAMRA) |
Description:
Free acid form of 5-carboxytetramethylrhodamine single
isomer. See Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solid soluble in DMSO, DMF, or MeOH
- C25H22N2O5.
- Mwt: 430.5
- [91809-66-4]
|
Chemical Structure of 90009
Reference: |
|
Catelog Number :
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90088 |
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Product Name :
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5-Carboxytetramethylrhodamine-PEO12-propionate
succinimidyl ester (5-TAMRA-PEO12 SE) |
Description:
5-TAMRA-PEO 12 SE has a long water-soluble PEG
spacer between tetramethylrhodamine and the aminereactive SE
group. See Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C56H78N4O20
- Mwt: 1127.23
|
Chemical Structure of 90088
Reference: |
|
Catelog Number :
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90087 |
|
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Product Name :
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5-Carboxytetramethylrhodamine-PEO8-propionate
succinimidyl ester (5-TAMRA-PEO8 SE) |
Description:
5-TAMRA-PEO 8 SE has a long water-soluble PEG
spacer between tetramethylrhodamine and the amine-reactive
SE group. See Figure 7.8 for spectra.
- lex\lemem
(MeOH) = 540/565 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C48H62N4O16
- Mwt: 951.02
|
Chemical Structure of 90087
Reference: |
|
Catelog Number :
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90036 |
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Product Name :
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5-Carboxy-X-rhodamine, succinimidyl ester (single isomer)(5-ROX,
SE) |
Description:
Amine-reactive form of 5-carboxy-X-rhodamine single isomer.
See Figure 7.9 for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C37H33N3O7.
- Mwt: 631.7
- [344402-35-3]
|
Chemical Structure of 90036
Reference: |
|
Catelog Number :
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|
90006 |
|
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Product Name :
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|
5-Carboxy-X-rhodamine, triethylammonium salt (single
isomer)(5-ROX) |
Description:
5-Carboxy-X-rhodamine is a single isomer, long wavelength
rhodamine dye. See Figure 7.9 for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO, DMF or MeOH
- Store at -20°C and protect from light
- C39H45N3O5.
- Mwt: 635.6
|
Chemical Structure of 90006
Reference: |
|
Catelog Number :
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|
90090 |
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Product Name :
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|
5-Carboxy-X-rhodamine-PEO12-propionate, succinimidyl
ester (5-ROX-PEO12 SE) |
Description:
5-ROX-PEO 12 SE has an approximately 47 Angstrom
water-soluble PEG spacer that separates the red fluorescent
ROX dye and the amine-reactive SE group. See Figure 7.9 for
spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO or DMF
- Store at ?20 °C and protect from light
- C64H86N4O20
- Mwt: 1231.38
|
Chemical Structure of 90090
Reference: |
|
Catelog Number :
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|
90089 |
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Product Name :
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|
5-Carboxy-X-rhodamine-PEO8-propionate, succinimidyl
ester (5-ROX-PEO8 SE) |
Description:
5-ROX-PEO 8 SE has an approximately 32 Angstrom
water-soluble PEG spacer that separates the red fluorescent
ROX dye and the amine-reactive SE group. See Figure 7.9 for
spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C56H70N4O16
- Mwt: 1055.17
|
Chemical Structure of 90089
Reference: |
|
Catelog Number :
|
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|
90093 |
|
|
Product Name :
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|
5-FAM-PEO12
SE (5-carboxyfluorescein-PEO12 propionate
succinimidyl ester) |
Description:
5-FAM-PEO 12 SE has an approximately 47 Angstrom
water-soluble PEG spacer that separates fluorescein and the
amine-reactive SE group. See Figure 7.4 for spectra.
- lex\lem(pH9)
= 494/521 nm
- Yellow orange solid soluble in DMF, DMSO, or pH 6.5
buffer.
- Store at -20°C and protect from light
- C52H68N2O22
- Mwt: 1073.10
|
Reference: |
|
Catelog Number :
|
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|
90085 |
|
|
Product Name :
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|
|
5-FAM-PEO8
SE (5-carboxyfluorescein-PEO8 propionate succinimidyl
ester) |
Description:
5-FAM-PEO 8 SE has an approximately 32 Angstrom
water-soluble PEG spacer that separates fluorescein and the
amine-reactive SE group. See Figure 7.4 for spectra.
- lex\lem(pH9)
= 494/521 nm
- Yellow orange solid soluble in DMF, DMSO, or pH 6.5
buffer.
- Store at -20°C and protect from light
- C44H52N2O18
- Mwt:896.89
|
Reference: |
|
Catelog Number :
|
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|
90095 |
|
|
Product Name :
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|
|
5-SFX
(6-(fluorescein-5-carboxamido)hexanoic acid, succinimidyl
ester)(single isomer) |
Description:
5-SFX has a 6-atom aminohexanoyl spacer group that separates
the fluorescein dye and the amine-reactive group. See Figure
7.4 for spectra.
- lex\lem(pH9)
= 494/521nm
- Yellow solid soluble in DMF, DMSO or pH>6.5 buffers
- Store at -20°C and protect from light
- C31H26N2O10
- Mwt:586.55
|
Chemical Structure of 90095
Reference: |
|
Catelog Number :
|
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|
90023 |
|
|
Product Name :
|
|
|
6-Carboxyfluorescein, succinimidyl ester (6-FAM, SE) (single
isomer) |
Description:
Amine-reactive form of 6-carboxyfluorescein single isomer.
See Figure 7.4 for spectra.
- lex\lem(pH
9) = 495/519 nm
- Yellow orang solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C25H15NO9
- Mwt: 473.4
|
Reference:
Bioconjugate Chem. 6, 447(1995) |
|
Catelog Number :
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|
90031 |
|
|
Product Name :
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|
6-Carboxyrhodamine
110, succinimidyl ester, single isomer (6-CR110, SE) |
Description:
Amine-reactive form of single isomer carboxyrhodamine 110.
For certain applications, the dye is preferred over the
corresponding 6-carboxyfluorescein because of its
exceptional photostability and fluorescence insensitivity to
pH change (4-9). See Figure 7.5 for spectra and Figure 7.6
for photostability comparison with other dyes.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C26H21N3O7
- Mwt: 489.4
|
Reference: |
|
Catelog Number :
|
|
|
90015 |
|
|
Product Name :
|
|
|
6-Carboxyrhodamine
110-X, succinimidyl ester (6-CR110-X, SE) |
Description:
Amine-reactive form of 6-carboxyrhodamine 110 with a
6-carbon spacer. See Figure 7.5 for spectra and Figure 7.6
for photostability comparison with other dyes.
- lex\lem(MeOH)
= 502/524 nm
- Orange red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C31H28N4O8
- Mwt: 584.58
|
Reference: |
|
Catelog Number :
|
|
|
90011 |
|
|
Product Name :
|
|
|
6-Carboxyrhodamine
6G, hydrochloride, single isomer (6-CR6G) |
Description:
Free acid form of 6-carboxyrhodamine 6G single isomer. See
Figure 7.7 for spectra.
- lex\lem(MeOH)
= 520/546 nm
- Orange red solid soluble in water (pH 7), DMF or
DMSO
- Store at -20°C and protect from light
- C27H27ClN2O5.
- Mwt: 494.97
|
Chemical Structure of 90011
Reference: |
|
Catelog Number :
|
|
|
90033 |
|
|
Product Name :
|
|
|
6-Carboxyrhodamine
6G, succinimidyl ester (single isomer)(6-CR6G SE) |
Description:
Amine-reactive form of 6-carboxyrhodamine 6G single isomer.
See Figure 7.7 for spectra.
- lex\lem(MeOH)
= 520/546 nm
- Orange red solid moderately soluble in DMSO
- Store at -20°C and protect from light
- C31H29ClN3O7
- Mwt: 555.6
|
Chemical Structure of 90033
Reference: |
|
Catelog Number :
|
|
|
90035 |
|
|
Product Name :
|
|
|
6-Carboxytetramethylrhodamine succinimidyl ester (single isomer)
(6-TAMRA SE) |
Description:
Amine-reactive form of 6-carboxytetramethylrhodamine single
isomer. See Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C29H25N3O7.
- Mwt: 527.5
- [150810-69-8]
|
Chemical Structure of 90035
Reference: |
|
Catelog Number :
|
|
|
90097 |
|
|
Product Name :
|
|
|
6-Carboxytetramethylrhodamine succinimidyl ester, 90mg/mL
(0.17M) in anhydrous DMSO (6-TAMRA SE in DMSO) |
Description:
Amine-reactive form of 6-TAMRA single isomer dissolved in
anhydrous DMSO. The solution is suitable for labeling
oligonucleotides. See Figure 7.8 for spectra. Also see
#90035 for molecular infomation.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solution
- Store desiccated at -20°C and protect from light
- C29H25N3O7
- Mwt: 527.5
|
Chemical Structure of 90097
Reference: |
|
Catelog Number :
|
|
|
90008 |
|
|
Product Name :
|
|
|
6-Carboxytetramethylrhodamine, single isomer (6-TAMRA) |
Description:
Free acid form of 6-carboxytetramethylrhodamine single
isomer. See Figure 7.8 for spectra.
- lex\lem(MeOH)
= 540/565 nm
- Dark red solid soluble in DMSO, DMF, MeOH, or pH>6.5
buffers
- C25H22N2O5.
- Mwt: 430.5
- [91809-67-5]
|
Chemical Structure of 90008
Reference: |
|
Catelog Number :
|
|
|
90013 |
|
|
Product Name :
|
|
|
6-Carboxy-X-rhodamine, free acid (6-ROX, free acid) |
Description:
6-Carboxy-X-rhodamine is a single isomer, long wavelength
rhodamine dye. See Figure 7.9 for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO, DMF or MeOH
- Store at -20°C and protect from light
- C33H30N2O5
- Mwt: 534.6
|
Reference: |
|
Catelog Number :
|
|
|
90037 |
|
|
Product Name :
|
|
|
6-Carboxy-X-rhodamine, succinimidyl ester (single isomer)(6-ROX,
SE) |
Description:
Amine-reactive form of 6-carboxy-X-rhodamine single isomer.
See Figure 7.9 for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C37H33N3O7.
- Mwt: 631.7
- [216699-36-4]
|
Chemical Structure of 90037
Reference: |
|
Catelog Number :
|
|
|
90098 |
|
|
Product Name :
|
|
|
6-Carboxy-X-rhodamine, succinimidyl ester, 83mg/mL (~0.13M) in
anhydrous DMSO (6-ROX, SE in DMSO) |
Description:
Amine-reactive form of 6-ROX single isomer dissolved in
anhydrous DMSO. The solution is suitable for labeling
oligonucleotides. See Figure 7.9 for spectra. See # 90037
for molecular information. |
Reference: |
|
Catelog Number :
|
|
|
90007 |
|
|
Product Name :
|
|
|
6-Carboxy-X-rhodamine, triethylammonium salt (single
isomer)(6-ROX) |
Description:
6-Carboxy-X-rhodamine is a single isomer, long wavelength
rhodamine dye. See Figure 7.9 for spectra.
- lex\lem(MeOH)
= 568/595 nm
- Dark red solid soluble in DMSO, DMF or MeOH
- Store at -20°C and protect from light
- C39H45N3O5.
- Mwt: 635.6
|
Chemical Structure of 90007
|
|
Catelog Number :
|
|
|
51020 |
|
|
Product Name :
|
|
|
6-FAM
(6-Carboxyfluorescein) |
Description:
Free acid form of 6-carboxyfluorescein single isomer. See
Figure 7.4 for spectra.
- lex\lem(pH
9) = 495/519 nm
- Yellow orange solid soluble in water (pH>6), DMF or
DMSO
- Store at 4°C and protect from light
- C21H12O7
- Mwt: 376.3
|
Reference: |
|
Catelog Number :
|
|
|
90094 |
|
|
Product Name :
|
|
|
6-JOE, SE |
Description:
6-JOE SE is a single isomer fluorescein derivative that has
been one of the traditional fluorophores used in automated
DNA sequencing. 1
- lex\lem(pH9)
= 522/550 nm
- Orange solid soluble in DMF, or DMSO
- Store at -20°C and protect from light
- C27H17Cl2NO11
- Mwt: 602.33
|
Reference:
1) Genome Res. 6, 1170(1996). |
|
Catelog Number :
|
|
|
90096 |
|
|
Product Name :
|
|
|
6-SFX
(6-(fluorescein-6-carboxamido)hexanoic acid, succinimidyl
ester)(single isomer) |
Description:
6-SFX has a 6-atom aminohexanoyl spacer group that separates
the fluorescein dye and the amine-reactive group. See Figure
7.4 for spectra.
- lex\lem
(pH9) = 494/521nm
- Yellow solid soluble in DMF, DMSO or pH>6.5 buffers
- Store at -20°C and protect from light
- C31H26N2O10
- Mwt:586.55
|
Reference: |
|
Catelog Number :
|
|
|
90077 |
|
|
Product Name :
|
|
|
AMCA-X, SE
(6-((7-amino-4-methylcoumarin-3-acetyl) amino)hexanoic acid,
succinimidyl ester) |
Description:
AMCA-X SE is an amine-reactive, UV-excitable, blue
fluorescent dye.
- lex\lem(MeOH)
= 353/442 nm
- White solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- C22H25N3O7
- Mwt: 443.45
|
Chemical Structure of 90077
Reference:
1) Angew Chem Int Ed Engl 40, 402(2001); 2) J Biol
Chem 276, 25236(2001); 3) J Immunol 164, 5236(2000). |
|
Catelog Number :
|
|
|
90025 |
|
|
Product Name :
|
|
|
BD405, SE |
Description:
Reference: |
|
Catelog Number :
|
|
|
90091 |
|
|
Product Name :
|
|
|
Eosin-5-isothiocyanate |
Description:
Eosin-5-isothiocyanate is a good photosensitizer and can be
used to selectively label amines. It has a quantum yield of
0.57 for singlet oxygen generation, which makes it useful
for photoconversion of electron-rich materials such as
diaminobenzidine (DAB) or fluorescent dyes into highly
electron-dense materials for high resolution electron
microscopy studies. 1,2 Additionally, eosin is a
good fluorescence acceptor for fluorescein (FAM), dansyl and
coumarin dyes in fluorescence resonance energy transfer
(FRET).
- lex\lem(pH9)
= 521/544 nm
- e = 95,000
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C21H7Br4NO5S
- Mwt: 704.96
|
Reference:
1) J. Cell Biol. 126, 901(1994); 2) J. Cell Biol.
126, 877(1994). |
|
Catelog Number :
|
|
|
90020 |
|
|
Product Name :
|
|
|
FITC (isomer I)
(fluorescein-5-isothiocyanate) |
Description:
FITC is an amine-reactive fluorescein derivative for
labeling proteins or other biomolecules containing primary
amines.
- lex\lem=
492/520nm
- e = 75,000
- Orange solid soluble in DMF or DMSO
- Store at -20? and protect from light, especially in
solution
- C21H11NO5S.
- Mwt: 389.4
- 3326-32-7]
|
Chemical Structure of 90020
Reference:
Cherry, N.B., et al. Stain Technol. 44, 179(1965). |
|
Catelog Number :
|
|
|
90092 |
|
|
Product Name :
|
|
|
Fluorescamine |
Description:
Fluorescamine is nonfluorescent until it reacts with primary
amines. The reaction is completed in miniseconds and can be
used to detect amine-containing compounds including peptides
and proteins (Figure 7.10). Unreacted fluorescamine is
rapidly converted to a nonfluorescent product by reaction
with water, making amine detection highly specific.
- lex\lem(CH3CN)
= 381/470 nm (for reaction product with amines)
- Off-white solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C17H10O4
- Mwt: 278.26
|
Reference:
1) Clin. Chim. Data 157, 73(1986); 2) J. Lipid Res.
27, 792(1986); 3) J. Chromatography 502, 427(1990). |
|
Catelog Number :
|
|
|
90045 |
|
|
Product Name :
|
|
|
NBD Chloride
(4-chloro-7-nitrobenzo-2-oxa-1,3-diazole) |
Description:
NBD chloride is nonfluorescent until it reacts with primary
or secondary amines to produce a fluorescent product. NBD
chloride has been extensively used as a derivatizing reagent
for chromatography analysis of amino acids and low molecular
weight amines.
- lex\lem(MeOH)
= 465/535 nm (for reaction product with primary amines);
485/540 nm (for reaction product with secondary amines)
- Yellow solid soluble in DMF
- Store at -20°C and protect from light
- C6H2ClN3O3
- Mwt: 199.55
|
Reference:
1) Biochem. J. 108, 155(1968); 2) Anal. Biochem. 116,
471(1981); 3) Anal. Chim. Acta 290, 3(1994). |
|
Catelog Number :
|
|
|
90046 |
|
|
Product Name :
|
|
|
NBD Fluoride
(4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole) |
Description:
NBD fluoride is similar to NBD chloride (# 90045)
except that the former is much more reactive toward amines.
Both reagents are nonfluorescent until they react with
primary or secondary amines to produce a fluorescent
product. NBD chloride and NBD fluoride have been extensively
used as derivatizing reagents for chromatography analysis of
amino acids and low molecular weight amines.
- lex\lem(MeOH)
= 465/535 nm (for reaction product with primary amines);
485/540 nm (for reaction product with secondary amines)
- Yellow solid soluble in DMF
- Store at -20°C and protect from light
- C6H2FN3O3
- Mwt:183
|
Reference:
1) Anal. Chim. Acta 130, 377(1981); 2) Biochem. J.
108, 155(1968); 3) Anal. Biochem. 116, 471(1981); 4) Anal. Chim. Acta
290, 3(1994). |
|
Catelog Number :
|
|
|
90021 |
|
|
Product Name :
|
|
|
Sulforhodamine 101
sulfonyl chloride (Texas Red sulfonyl chloride) |
Description:
Sulforhodamine 101 sulfonyl chloride (also called Texas Red
sulfonyl chloride) is a popular long wavelength
amine-reactive rhodamine dye. Because the dye is quite
unstable in water, reactions with amines in an aqueous
solution must be carried out at low temperature (over ice or
at 4°C) and usually at pH 8.5. Biotium developed
sulforhodamine 101-PEO 2 SE (# 90039)
as a superior alternative to sulforhodamine 101 sulfonyl
chloride because the dye has a water-soluble spacer group
and is also much more stable.
- lex\lem=
583/603nm
- Dark purple solid soluble in DMF (Caution: Do not
dissolve in DMSO)
- Store at -20°C and protect from moisture and light
- 31H29ClN2O6S2.
- Mwt: 625.16
- [82354-19-6]
|
Chemical Structure of 90021
Reference:
J. Immunol. Meth. 50, 193(1982). |
|
Catelog Number :
|
|
|
90100 |
|
|
Product Name :
|
|
|
Sulforhodamine
101-PEO12-propionic acid succinimydyl ester
(SR101-PEO12 SE, or Texas Red-PEO12 SE) |
Description:
SR101-PEO 12 SE has an approximately 47 Angstrom
water-soluble PEG spacer that separates the dye from the
amine-reactive SE group. See Figure 7.12 for spectra.
- lex\lem=
583/603nm
- Dark purple solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C62H86N4O22S2
- Mwt: 1303.49
|
Reference: |
|
Catelog Number :
|
|
|
90039 |
|
|
Product Name :
|
|
|
Sulforhodamine
101-PEO2-propionic acid succinimydyl ester (SR101-PEO2-P
SE, or Texas Red-PEO2-P SE) |
Description:
SR101-PEO 2 SE has a water-soluble PEG spacer that
separates the dye from the aminereactive SE group. The dye
is a superior alternative to the popular amine-reactive dye
sulforhodamine 101 sulfonyl chloride (also called Texas
RedTM sulfonyl chloride). The water-soluble spacer should
minimize the interaction between the dye and the biomolecule
to be labeled. See Figure 7.12 for spectra.
- lex\lem=
583/603nm
- Dark purple solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C42H46N4O12S2
- Mwt: 862.26
|
Reference: |
|
Catelog Number :
|
|
|
90099 |
|
|
Product Name :
|
|
|
Sulforhodamine
101-PEO8-propionic acid succinimydyl ester (SR101-PEO8
SE, or Texas Red-PEO8 SE) |
Description:
SR101-PEO 8 SE has an approximately 32 Angstrom
watersoluble PEG spacer that separates the dye from the
aminereactive SE group. See Figure 7.12 for spectra.
- lex\lem=
583/603nm
- Dark purple solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C54H70N4O18S2
- Mwt: 1127.28
|
Reference: |
|
Catelog Number :
|
|
|
90042 |
|
|
Product Name :
|
|
|
Sulforhodamine
B-EO-propionic acid succinimydyl ester (SRB-EO-P SE) |
Description:
Reference: |
|
Catelog Number :
|
|
|
90102 |
|
|
Product Name :
|
|
|
Sulforhodamine
B-PEO12-propionic acid succinimydyl ester (SRB-PEO12
SE) |
Description:
SR101-PEO 12 SE has an approximately 47 Angstrom
water-soluble PEG spacer that separates the dye from the
amine-reactive SE group.
- lex\lem=
560/580nm
- Dark purple solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C58H86N4O22S2
- Mwt: 1255.45
|
Reference: |
|
Catelog Number :
|
|
|
90043 |
|
|
Product Name :
|
|
|
Sulforhodamine
B-PEO2-propionic acid succinimydyl ester (SRB-PEO2-P
SE) |
Description:
SRB-PEO 2 SE has a water-soluble PEG spacer that
separates the dye sulforhodamine B (also called Lisamine
Rhodamine) from the amine-reactive SE group. The dye is a
superior alternative to the popular amine-reactive dye
sulforhodamine B sulfonyl chloride. The water-soluble spacer
should minimize the interaction between the dye and the
biomolecule to be labeled.
- lex\lem=
560/580nm
- Dark purple solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C38H46N4O12S2
- Mwt: 814.92
|
Reference: |
|
Catelog Number :
|
|
|
90101 |
|
|
Product Name :
|
|
|
Sulforhodamine
B-PEO8-propionic acid succinimydyl ester (SRB-PEO8
SE) |
Description:
SR101-PEO 8 SE has an approximately 32 Angstrom
water-soluble PEG spacer that separates the dye from the
amine-reactive SE group.
- lex\lem=
560/580nm
- Dark purple solid soluble in DMF or DMSO
- Store at -20°C and protect from light, especially in
solution
- C50H70N4O18S2
- Mwt: 1079.24
|
Reference: |
|
Catelog Number :
|
|
|
90018 |
|
|
Product Name :
|
|
|
Tetramethylrhodamine 5-isothiocyanate chloride (5-TRITC,
Gisomer) |
Description:
5-TRITC is spectrally similar to TAMRA ( #90022,
#90034
and #90035).
The isothiocyanate reactive group reacts with primary or
secondary amines to form a thiourea bond linkage.
- lex\lem=
544/572nm
- Dark purple solid soluble in DMF
- Store at -20°C and protect from moisture and light
- C25H22ClN3O3S
- Mwt: 479.98
|
Reference: |
|
Catelog Number :
|
|
|
90019 |
|
|
Product Name :
|
|
|
Tetramethylrhodamine 6-isothiocyanate chloride (6-TRITC,
Risomer) |
Description:
6-TRITC is spectrally similar to TAMRA (# 90022,
# 90034
and # 90035). The isothiocyanate
reactive group reacts with primary or secondary amines to
form a thiourea bond linkage.
- lex\lem=
544/572nm
- Dark purple solid soluble in DMF
- Store at -20°C and protect from moisture and light
- C25H22ClN3O3S
- Mwt: 479.98
|
Reference: |
|
Catelog Number :
|
|
|
90017 |
|
|
Product Name :
|
|
|
Tetramethylrhodamine isothiocyanate chloride (5(6)-TRITC,mixed
isomers) |
Description:
5(6)-TRITC is spectrally similar to TAMRA (# 90022,
# 90034 and # 90035).The
isothiocyanate reactive group reacts with primary or
secondary amines to form a thiourea bond linkage.
- lex\lem=
544/572nm
- Dark purple solid soluble in DMF
- Store at -20°C and protect from moisture and light
- C25H22Cl N3O3S
- Mwt: 479.98
|
Reference: |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45