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Alkanethiosulfonate and Thiosulfate Probes for Selective Thiol Labeling
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Catelog Number :
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91032 |
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Product Name :
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2-((4-Azido-2,3,5,6-tetrafluorobenzoyl)amino)ethyl
methanethiosulfonate |
Description:
2-((4-Azido-2,3,5,6-tetrafluorobenzoyl)amino)ethyl
methanethiosulfonate is a cross-linker containing a
thiol-reactive MTS group and a photoaffinity label
4-azido-2,3,5,6-tetrafluorobenzene (ATFB). ATFB is known to
be a superior photoaffinity label that undergoes highly
efficient C H bond insertion on UV photolysis.1 Figure 7.2
illustrates the mechanism of ATFB photoaffinity labeling.
- White solid soluble in DMF or DMSO.
- Store at -20°C and protect from light.
- C10H8F4N4O3S2
- Mwt: 372.32
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Chemical Structure of 91032
Reference:
1) J. Org. Chem. 55, 3640(1990). |
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Catelog Number :
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91044 |
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Product Name :
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3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate |
Description:
3,6,9,12-Tetraoxatetradecane-1,14-diyl
dimethanethiosulfonate is a water soluble MTS cross-linker.
Stock solution made in anhydrous DMF is good for at least
two months if stored at -20°C. However, stock solution made
in water or a buffer should be used immediately.
- Off-white solid soluble in DMF or water
- Store desiccated at -20°C and protect from light
- C12H26O8S4
- Mwt: 426.57
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Chemical Structure of 91044
Reference: |
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Catelog Number :
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91043 |
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Product Name :
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3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate |
Description:
3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a
water soluble MTS cross-linker. Stock solution made in
anhydrous DMF is good for at least two months if stored at
-20°C. However, stock solution made in water or a buffer
should be used immediately.
- Off-white solid soluble in DMF or water
- Store desiccated at -20°C and protect from light
- C10H22O7S4
- Mwt: 382.52
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Chemical Structure of 91043
Reference: |
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Catelog Number :
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91042 |
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Product Name :
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3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate |
Description:
3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water
soluble MTS cross-linker. Stock solution made in anhydrous
DMF is good for at least two months if stored at -20°C.
However, stock solution made in water or a buffer should be
used immediately.
- Off-white liquid soluble in DMF or water
- Store desiccated at -20°C and protect from light
- C8H18O6S4
- Mwt: 338.49
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Chemical Structure of 91042
Reference: |
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Catelog Number :
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91031 |
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Product Name :
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AEAETS
(2-aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) |
Description:
AEAETS is a doubly positively charged alkanethiosulfonate.
- White solid soluble in water, DMSO and DMF.
- Store at -20°C and protect from light.
- C4H14Cl2N2O2S2
- Mwt: 257
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Chemical Structure of 91031
Reference:
1)J. Gen. Physiol. 115, 93(2000); 2) Meth. Enzymol.
293, 123(1998). |
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Catelog Number :
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91045 |
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Product Name :
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BisEA
(bis-(2-(methanethiosulfonato)ethyl)amine, hydrochloride) |
Description:
BisEA is a positively charged MTS cross-linker.
- Off-white solid soluble in DMF or DMSO
- Store desiccated at -20°C
- C6H16ClNO4S4
- Mwt: 329.91
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Chemical Structure of 91045
Reference:
1) Hastrup, Karlin, & Javitch. Proc. Natl. Acad. Sci.
USA, 98(18), 10055(2001). |
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Catelog Number :
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91034 |
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Product Name :
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CPMTS
(7-diethylamino-4-methyl-3-(4-(4-(methanethiosulfonato)butanoyl)amino)phenylcoumarin) |
Description:
Biotium developed CPMTS as an alternative to the widely used
thiol-reactive coumarin dye CPM (# 91010).
CPMTS and CPM have nearly identical absorption and emission
spectra. However, CPMTS contains a MTS group, which could
make the dye more advantageous than CPM for sulfhydryl
labeling because of the high selectivity and reactivity of
MTS reagents toward thiol groups.
- Off-white solid soluble in DMF or DMSO
- Store desiccated at -20°C and protect from light
- C25H30N2O5S2
- Mwt: 502.65
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Chemical Structure of 91034
Reference: |
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Catelog Number :
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91033 |
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Product Name :
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MTS-AMCA |
Description:
AMCA (7-amino-4-methylcoumarin-3-acetic acid) is one of the
most widely used UV-excitable, blue fluorescent dye.
MTS-AMCA readily labels a thiol with AMCA.
- lex\lem=
353/442 nm
- Off-white solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C15H18N2O5S2.
- Mwt: 370.45
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Chemical Structure of 91033
Reference: |
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Catelog Number :
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91026 |
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Product Name :
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MTSBS
(4-sulfonatobutyl methanethiosulfonate sodium salt) |
Description:
MTSBS is a negatively charged MTS reagent.
- White solid soluble in water, DMSO or DMF
- Store desiccated at -20°C
- C5H11NaO5S3
- Mwt: 270
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Chemical Structure of 91026
Reference: |
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Catelog Number :
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91035 |
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Product Name :
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MTS-CR110
(MTS-5-(and-6)-carboxyrhodamine 110, mixed isomers) |
Description:
MTS-CR110 is a green fluorescent thiol-reactive dye. CR110
(5-(and-6)- carboxyrhodamine 110) has absorption and
emission spectra (502/524 nm) similar to those of
fluorescein (495/519 nm) but its photostability is far
better than that of the latter. Thus, MTS-CR110 is a
superior green fluorescent dye for labeling thiols.
- lex\lem(MeOH)
= 502/524 nm
- Orange solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C24H21N3O6S2.
- Mwt: 511
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Chemical Structure of 91035
Reference: |
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Catelog Number :
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91036 |
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Product Name :
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MTS-CR110-X
(MTS-5-(and-6)-carboxyrhodamine 110-X, mixed isomers) |
Description:
MTS-CR110-X is a green fluorescent thiol-reactive dye. It is
similar to MTSCR110 (# 91035) except
that the former has a X spacer molecule between the dye and
the MTS reactive group. The linker molecule may enhance
fluorescence when the dye is attached to proteins.
- lex\lem(MeOH)
= 502/524 nm
- Orange solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C30H32N4O7S2.
- Mwt: 624
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Chemical Structure of 91036
Reference: |
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Catelog Number :
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91022 |
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Product Name :
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MTSEA (2-aminoethyl
methanethiosulfonate hydrobromide) |
Description:
MTSEA is a positively charged MTS reagent.
- Off-white solid soluble in water, DMSO or DMF
- Store desiccated at -20°C
- C3H10BrNO2S2
- MWt: 236.15
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Chemical Structure of 91022
Reference:
1) Biochemistry 33, 6840(1994);
2) Science 258, 307(1992). |
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Catelog Number :
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90064 |
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Product Name :
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MTSEA biotin
(2-((biotinoyl)amino)ethyl methanethiosulfonate) |
Description:
MTSEA biotin selectively labels thiols under mild condition.
Please also see our TS biotin ethylenediamine (# 92010),
TS biotin-X ethylenediamine (# 92011)
and TS biotin- XX ethylenediamine (# 92012),
all of which allow selective labeling of cell surface
thiols.
- White solid soluble in DMF, or DMSO at =10 mg/mL
- Store desiccated at -20°C
- C13H23N3O4S3
- Mwt: 381.52
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Chemical Structure of 90064
Reference:
1) J Membr Biol, 180(1), 83 (2001); 2) J Neurosci,
21(1), 67 (2001); 3) J Biol Chem, 2000 Oct 31; 4) J Neurosci, 19(12),
4847 (1999); 5) J Biol Chem, 273(20), 12675 (1998) |
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Catelog Number :
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90064-1 |
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Product Name :
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MTSEA biotin
(2-((biotinoyl)amino)ethyl methanethiosulfonate) |
Description:
See # 91064 for product description. |
Chemical Structure of 90064-1
Reference: |
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Catelog Number :
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90065-1 |
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Product Name :
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MTSEA biotin-X
(MTSEA BIOTINCAP, or
2-((6-((biotinoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate)) |
Description:
See # 90065 for product description. |
Chemical Structure of 90065-1
Reference: |
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Catelog Number :
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90065 |
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Product Name :
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MTSEA biotin-X
(MTSEA BIOTINCAP, or
2-((6-((biotinoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate)) |
Description:
MTSEA biotin-X selectively labels thiols under mild
condition. The X-spacer should facilitate biotin-avidin
interaction. Please also see TS biotin ethylenediamine (# 92010),
TS biotin-X ethylenediamine (# 92011)
and TS biotin-XX ethylenediamine (# 92012),
all of which allow selective labeling of cell surface
thiols.
- White solid soluble in DMF, or DMSO at =10 mg/mL
- Store desiccated at -20°C
- C19H34N4O5S3
- Mwt: 494.68
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Chemical Structure of 90065
Reference:
1) J Membr Biol, 180(1), 83 (2001); 2) J Neurosci,
21(1), 67 (2001); 3) J Biol Chem, 2000 Oct 31; 4) J Neurosci, 19(12),
4847 (1999); 5) J Biol Chem, 273(20), 12675 (1998) |
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Catelog Number :
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90066 |
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Product Name :
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MTSEA biotin-XX
(MTSEA BIOTINCAPCAP, or
2-((6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate) |
Description:
O MTSEA biotin-XX selectively labels thiols under mild
condition. The XX-linker should facilitate biotin-avidin
interaction. Please also see TS biotin ethylenediamine (# 92010),
TS biotin-X ethylenediamine (# 92011)
and TS biotin-XX ethylenediamine (# 92012),
all of which allow selective labeling of cell surface
thiols.
- White solid soluble in DMF, or DMSO at =10 mg/mL
- Store desiccated at -20°C
- C25H45N5O6S3
- Mwt: 607.7
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Chemical Structure of 90066
Reference:
1) J Membr Biol, 180(1), 83 (2001); 2) J Neurosci,
21(1), 67 (2001); 3) J Biol Chem, 2000 Oct 31; 4) J Neurosci, 19(12),
4847 (1999); 5) J Biol Chem, 273(20), 12675 (1998) |
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Catelog Number :
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90066-1 |
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Product Name :
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MTSEA biotin-XX
(MTSEA BIOTINCAPCAP, or
2-((6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate) |
Description:
See # 90066 for product description. |
Chemical Structure of 90066-1
Reference: |
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Catelog Number :
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91023 |
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Product Name :
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MTSEH
(2-hydroxyethyl methanethiosulfonate) |
Description:
MTSEH is a neutral MTS reagent.
- Colorless liquid soluble in DMSO or DMF
- Store desiccated at -20°C and protect from light
- C3H8O3S2
- Mwt: 156
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Chemical Structure of 91023
Reference:
1)I. Gen. Physiol. 115, 93(2000); 2) Meth. Enzymol.
293, 123(1998). |
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Catelog Number :
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91020 |
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Product Name :
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MTSES
(2-sulfonatoethyl methanethiosulfonate sodium salt) |
Description:
MTSES is a negatively charged MTS reagent.
- White solid soluble in DMSO
- Store desiccated at -20°C
- C3H7NaO5S3
- MWt: 242.21
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Chemical Structure of 91020
Reference:
1) Biochemistry 33, 6840(1994); 2) Science 258,
307(1992). |
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Catelog Number :
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91021 |
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Product Name :
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MTSET
(2-(trimethylammonium)ethyl methanethiosulfonate, Bromide) |
Description:
MTSET is a positively charged MTS reagent.
- Off-white solid soluble in water or DMSO
- Store desiccated at -20°C
- C6H16BrNO2S2
- MWt: 278.23
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Chemical Structure of 91021
Reference:
1) Biochemistry 33, 6840(1994); 2) Science 258,
307(1992). |
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Catelog Number :
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91024 |
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Product Name :
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MTSPA
(3-aminopropyl methanethiosulfonate hydrobromide) |
Description:
MTSPA is a positively charged MTS reagent.
- White solid soluble in water, DMSO or DMF
- Store desiccated at -20°C and protect from light
- C4H12BrNO2S2
- Mwt: 250.15
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Chemical Structure of 91024
Reference: |
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Catelog Number :
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91025 |
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Product Name :
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MTSPS
(3-sulfonatopropyl methanethiosulfonate sodium salt) |
Description:
MTSPS is a negatively charged MTS reagent.
- White solid soluble in water, DMSO or DMF
- Store desiccated at -20°C and protect from light
- C4H9NaO5S3
- Mwt: 256
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Chemical Structure of 91025
Reference: |
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Catelog Number :
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91027 |
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Product Name :
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MTSPT
(3-trimethylammoniumpropyl methanethiosulfonate bromide salt) |
Description:
MTSPT is a positively charged MTS reagent.
- White solid soluble in water, DMSO or DMF
- Store desiccated at -20°C and protect from light
- C7H18BrNO2S2
- Mwt: 292
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Chemical Structure of 91027
Reference: |
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Catelog Number :
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91038 |
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Product Name :
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MTS-Rhodamine 6G |
Description:
MTS-Rhodamine 6G is a fast-reacting and highly selective
thiol-reactive rhodamine dye.
- lex\lem(MeOH)
= 520/546 nm
- Orange red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C30H33N3O6S2.
- Mwt: 595
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Chemical Structure of 91038
Reference: |
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Catelog Number :
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91037 |
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Product Name :
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MTS-ROX , mixed
isomers (MTS-5(6)-carboxy-X-rhodamine) |
Description:
MTS-ROX is a fast-reacting and highly selective
thiol-reactive rhodamine dye.
- lex\lem(MeOH)
= 568/595 nm
- Dark Red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C36H37N3O6S2.
- Mwt: 671.8
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Chemical Structure of 91037
Reference: |
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Catelog Number :
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91048 |
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Product Name :
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MTS-Sulforhodamine
101 (MTS-Texas Red) |
Description:
MTS-sulforhodamine 101 is a fast-reacting and highly
selective thiol-reactive rhodamine dye.
- lex\lem(MeOH)
= 583/603 nm
- Dark Red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C34H37N3O8S4.
- Mwt: 743.94
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Reference: |
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Catelog Number :
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91030 |
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Product Name :
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MTS-TAMRA , mixed
isomers (MTS 5(6)-carboxytetramethylrhodamine) |
Description:
MTS-TAMRA is a fast-reacting and highly selective
thiol-reactive label that permits one to attach the popular
fluorescent dye carboxytetramethylrhodamine to a thiol
group.
- lex\lem(MeOH)
= 540/565 nm
- Red solid soluble in DMF or DMSO
- Store at -20°C and protect from light
- C28H29N3O6S2.
- Mwt: 567
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Chemical Structure of 91030
Reference: |
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Catelog Number :
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92010 |
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Product Name :
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TS Biotin
ethylenediamine (biotin ethylenediamine thiosulfate,sodium salt) |
Description:
TS biotin ethylenediamine is a highly selective thiol
labeling probe. Similar to the MTS group, TS (thiosulfate)
group is capable of reacting with thiols at nearly neutral
pH. However, unlike the MTS group , which is neutral, TS is
negatively charged and thus probes bearing a TS group are
ideal for specifically labeling thiols exposed at the
exterior cell surface because charged probes are less likely
to enter cells.
- Off-white liquid soluble in DMSO or water
- Store desiccated at -20°C
- C14H23N4NaO6S3
- Mwt: 462.54
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Chemical Structure of 92010
Reference: |
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Catelog Number :
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92011 |
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Product Name :
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TS Biotin-X
ethylenediamine (biotin-X ethylenediamine thiosulfate,sodium
salt) |
TS biotin ethylenediamine is a highly selective thiol
labeling probe. Similar to the MTS group, TS (thiosulfate)
group is capable of reacting with thiols at nearly neutral
pH. However, unlike the MTS group , which is neutral, TS is
negatively charged and thus probes bearing a TS group are
ideal for specifically labeling thiols exposed at the
exterior cell surface because charged probes are less likely
to enter cells. The X spacer should facilitate the
interaction between biotin and avidin or streptavidin.
- Off-white liquid soluble in DMSO or water
- Store desiccated at -20°C
- C20H34N5NaO7S3
- Mwt: 575.70
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Chemical Structure of 92011
Reference: |
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Catelog Number :
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92012 |
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Product Name :
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TS Biotin-XX
ethylenediamine (biotin-XX ethylenediamine thiosulfate, sodium
salt) |
Description:
TS biotin ethylenediamine is a highly selective thiol
labeling probe. Similar to the MTS group, TS (thiosulfate)
group is capable of reacting with thiols at nearly neutral
pH. However, unlike the MTS group , which is neutral, TS is
negatively charged and thus probes bearing a TS group are
ideal for specifically labeling thiols exposed at the
exterior cell surface because charged probes are less likely
to enter cells. The XX spacer should facilitate the
interaction between biotin and avidin or streptavidin.
- Off-white liquid soluble in DMSO or water
- Store desiccated at -20°C
- C26H45N6NaO8S3
- Mwt: 688.86
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Chemical Structure of 92012
Reference: |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45