Reagents for Molecular Biology
Agarose (Molecular Grade)
Agarose Tablets (Molecular
Grade)
Co-Precipitants
Half-Dye Mix
Human Genomic DNA
IPTG
Rat Genomic DNA
Sheared DNA for Hybridisation
Sure Clean
Sure Clean Plus
X-GAL
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Agarose (Molecular
Grade) |
100g
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|
BIO-41026
|
1
|
|
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| Agarose (Molecular
Grade) |
500g
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|
BIO-41025
|
1
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- Features
• Excellent value and good
clarity.
• High gel strength
(>1500g/cm2).
• Free from DNase and RNase.
- Description
Bioline agarose is a
high-purity, molecular-grade
agarose free from DNase and
RNase contamination. The
high gel strength means that
gels as low as 0.5% are
practicable.
- Applications
• Ideal for separation of a
wide size range of nucleic
acids, especially large
fragments (>10kb).
- Storage Conditions
Agarose should be stored at
room temperature in dry
conditions.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Agarose Tablets
(Molecular Grade) |
150g
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BIO-41028
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1
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| Agarose Tablets
(Molecular Grade) |
300g
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BIO-41027
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1
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- Features
-
 •
Convenient and time saving
• Greater gel-to-gel
consistency
• Safer and cleaner to use
• DNase and RNase free
- Description
- Bioline’s new no-mess
Agarose Tablets represents a
new advance in convenience
products for the laboratory.
Each tablet contains a
pre-determined amount of
agarose (0.5g agarose per
tablet), removing the need
to weigh out loose agarose
powder. Agarose Tablets
require 5-6 minutes
incubation at room
temperature in buffer prior
to heating*. This incubation
step softens and the
dissolves the tablet in the
buffer. Once dissolved,
proceed as normal, heating
the agarose solution,
preferably using a
microwave. Bioline Agarose
Tablets have the same
specification as Bioline
Molecular Grade Agarose
powder and are suitable for
all routine applications.
* The time needed to
dissolve the tablet is
dependant on the laboratory
temperature. Please, do not
microwave the tablet in the
buffer immediately, as this
will cause formation of
smaller insoluble pellets.
The room temperature
incubation step, prior to
heating, is important to
achieve a homogeneous mix.
- Applications
- • Ideal for separation
of a wide size range of
nucleic acids.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Co-Precipitants |
1.5ml
|
Colourless 5mg/ml
|
BIO-37074
|
1
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| Co-Precipitants |
1.5ml
|
Pink 5mg/ml
|
BIO-37075
|
1
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| Co-Precipitants |
1.5ml
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Orange 5mg/ml
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BIO-37076
|
1
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| Co-Precipitants |
1ml
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Glycogen 20mg/ml
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BIO-37077
|
1
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- Features
- Aids precipitation
of nucleic acids, giving
almost 100% recovery of
as low as 25bp
fragments.
- Suitable for
sequencing.
- Free of DNA, RNA or
protein.
- Supplied with buffer
for optimum performance.
- Increases pellet
mass and visibility of
sample pellet.
- Minimises pellet
loss during sub-sequent
downstream applications.
- Description
Visible DNA pellets.
Bioline has introduced a
range of Co-Precipitants:
Glycogen (Isolated from
Oyster) and Linear
Polyacrylamides (colourless,
pink and orange) to aid
recovery of nucleic acids
during salt/alcohol
precipitations. Using a
simple and rapid procedure,
Co-Precipitants can be used
for the quantitative
recovery of small amounts of
nucleic acids within dilute
solutions, and are suitable
for most applications,
including precipitation of
DNA for sequencing and after
enzymatic manipulations, as
well as the precipitation of
RNA from different sources.
Specially treated
Co-Precipitants do not
contain detectable amounts
of nucleic acids, thus
allowing the use of
precipitates for standard
and RT-PCR and other
enzymatic reactions, that
may otherwise be inhibited
by untreated polysaccharide
alternatives.
- Applications
Recovery of DNA and RNA for
sequencing and other
enzymatic treatment.
- Notes
Glycoprecipitant is free of
background polynucleotides,
DNA and RNA-binding
proteins, proteases, DNases
and RNases. However, some
enzymatic reactions may be
inhibited by the presence of
polysaccharides.
Colourless Polyacrylamide is
the only Co-Precipitant
suitable for spectral
determination of nucleic
acid within the 260nm-280nm
range.
- Storage Conditions
Co-Precipitants can be
stored for 18 months at
-20°C in a
constant-temperature
freezer.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Half-Dye Mix |
~250 templates
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BIO-36025
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1
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| Half-Dye Mix |
~1000 templates
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BIO-36026
|
1
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- Product Description
- Half-Dye Mix has been
designed to reduce the costs
involved in auto-sequencing,
by reducing the amount of
dye-terminator needed in a
reaction. Many sequencing
reactions leave up to 80% of
dye-terminators unused,
which normally require
removal prior to sequencing.
Half-Dye Mix addresses this
inefficiency, by providing
the necessary reaction
conditions to allow for a
lower proportion of
dye-terminator in the
reaction mix, hence reducing
the amount of unused dye
terminators that need
removal, and thus allowing
many more reactions from
every purchase of
dye-terminator.
It works by providing
optimal buffer conditions
which allow up to a 5-fold
dilution of dye-terminator
without any loss in
sequencing quality. Half-dye
is suitable for sequencing
of both Plasmid and PCR
templates and requires no
optimisation.
- Features
- • Reduces requirement
for BigDye(8)
• Half-Dye Mix can reduce
auto-sequencing costs
significantly
• Supplied in ready-to-use
format
- Applications
- • Autosequencing.
- Plasmid Template
-
1 in 3 dilution of Big Dye
terminator mix with Half-Dye
- PCR Template
-
1 in 5 dilution of Big Dye
terminator mix with Half-Dye
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
|
QUANTITY
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| Human Genomic DNA |
500µl
|
200ng/µl
|
BIO-35025
|
1
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- Features
-
- Highly purified.
- Fragments are >50Kb.
- Description
Human Genomic DNA is highly
purified and isolated human
placenta. The average length
of the DNA is greater than
50Kb and is suitable for
Southern blotting, genomic
library construction and as
a control template.
- Applications
-
- Southern blotting.
- Genomic library
construction.
- Control template.
- References
Sambrook, J., Fritisch, E.
F. and Maiatis, T. (1989)
Molecular Cloning: A
Laboratory Manual, 2nd ed.,
Cold Spring Harbor
Laboratory, Cold Spring
Harbor, NY, 9.14.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| IPTG |
5g
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BIO-37036
|
1
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- Features
• Induces activity of the
E.coli lac operon by binding
and inhibiting the lac
repressor.
• Offers one of the highest
levels of purity available
among commerc- ially
available IPTG (99.6% by
HPLC).
- Description
Isopropyl
b-D-thiogalactopyranoside
(IPTG) is a chemical
analogue of galactose. Genes
controlled by the lac or tac
promoter/operator sequences
are expressed to high levels
in the presence of IPTG.
- Applications
• Blue/white-colony
screening.
• Induction of lac operon
for protein expression.
- Assay
99.6% by HPLC.
- Formula
C9H18O5S
- Molecular Weight
238.3
- Storage Conditions
When stored at –20°C, in a
constant-temperature freezer
protected from light, IPTG
will remain stable.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Rat Genomic DNA |
500µl
|
200ng/µl
|
BIO-35026
|
1
|
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|
- Features
-
- Highly purified.
- Fragments are >50Kb.
- Description
Rat Genomic DNA is highly
purified and isolated from
Rattus norvegicus brain
tissue. The average length
of the DNA is greater than
50Kb and is suitable for
Southern blotting, genomic
library construction and as
a control template.
- Applications
-
- Southern blotting.
- Genomic library
construction.
- Control template.
- References
Sambrook, J., Fritisch, E.
F. and Maiatis, T. (1989)
Molecular Cloning: A
Laboratory Manual, 2nd ed.,
Cold Spring Harbor
Laboratory, Cold Spring
Harbor, NY, 9.14.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Sheared DNA for
Hybridisation |
5ml
|
10mg/ml solution in 1xTE buffer.
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BIO-37078
|
1
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|
|
- Features
-
- Free from RNase and
DNase.
- Ready-to-go
solution.
- Description
For blot blocking.
Molecular-Biology Grade
Solution of sheared DNA for
direct use as a blocking
agent in Southern and
Northern blotting
experiments in order to
prevent unspecific DNA
hybridisation. Sheared DNA
is prepared from fish sperm
(Cod), treated with SDS and
proteinase K and extracted
with Phenol/Chloroform. DNA
fragments are smaller than
1Kb.
- Applications
Southern and Northern
hybridisation.
- Quality Control Tests
Molecular weight size,
absence of DNase, RNase
activities.
- Storage Conditions
The product can be stored
for 12 months at -20ºC in a
constant-temperature
freezer.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| Sure Clean |
5ml
|
N/A
|
BIO-37042
|
1
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| Sure Clean |
25ml
|
N/A
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BIO-37046
|
1
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- Features
-
- Column-free PCR
Cleanup
- Recovery rates of up
to 98%.
- Optional* use of
Pink Co-Precipitant
which allows easy
visualization of DNA
Pellet.
- Suitable for DNA,
cDNA, or dsRNA
purification.
- Removes primers,
primer-dimers,dNTPs,
restriction enzymes
efficiently.
- Purified nucleic
acids can be used
immediately for
downstream applications
such as cloning and
sequencing.
- Description
SureClean is a novel,
inexpensive solution
which provides a
column-free method for
nucleic-acid
purification. Using a
simple and rapid
procedure, SureClean can
be used to purify or
concentrate DNA or dsRNA
from PCR reactions or
any enzymatic digests.
The simple method
combines convenience,
speed and excellent
recovery rates.
SureClean Plus
incorporates a
pink-precipitant that
offers the distinct
advantage of easy
visualisation of the
purified pellet, which
acquires a pink colour.
- Applications
-
- Purify DNA or
dsRNA
- Maximise
recovery
- Remove primers
- Resuspend
visualised product
in buffer and volume
of your choice
- Purified DNA can
be subsequently
sequenced or ligated
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
|
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| Sure Clean Plus |
5ml
|
N/A
|
BIO-37047
|
1
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| Sure Clean Plus |
25ml
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N/A
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BIO-37048
|
1
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- Features
-
- Column-free PCR
Cleanup
- Recovery rates of up
to 98%.
- Optional* use of
Pink Co-Precipitant
which allows easy
visualization of DNA
Pellet.
- Suitable for DNA,
cDNA, or dsRNA
purification.
- Removes primers,
primer-dimers,dNTPs,
restriction enzymes
efficiently.
- Purified nucleic
acids can be used
immediately for
downstream applications
such as cloning and
sequencing.
- Product
Description
SureClean is a novel,
inexpensive solution
which provides a
column-free method for
nucleic-acid
purification. Using a
simple and rapid
procedure, SureClean can
be used to purify or
concentrate DNA or dsRNA
from PCR reactions or
any enzymatic digests.
The simple method
combines convenience,
speed and excellent
recovery rates.
SureClean Plus
incorporates a
pink-precipitant that
offers the distinct
advantage of easy
visualisation of the
purified pellet, which
acquires a pink colour.
- Applications
-
- Purify DNA or
dsRNA
- Maximise
recovery
- Remove primers
- Resuspend
visualised product
in buffer and volume
of your choice
- Purified DNA can
be subsequently
sequenced or ligated
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| X-GAL |
1g
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BIO-37035
|
1
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- Features
• White, crystalline powder
yielding a blue precipitate
upon hydrolysis.
• Offers an unrivalled
purity of 99.5%, single spot
determined by TLC
- Description
5-bromo-4-chloro-3-indolyl
ß-D-galactopyranoside
(X-Gal) is a chromogenic
substrate for
ß-Galactosidase that forms
an intense blue precipitate.
It can be used in Molecular
Biology to detect the gal
gene product, and also in
Microbiology, where it is
used to detect
micro-organisms which have
ß-Galactosidase activity
(usually coliforms). It can
be combined with the
R-substrates to
differentiate between two
species of organisms on the
same plate. X-Gal is soluble
in N, N-dimethylformamide.
- Assay
98.5% single spot determined
by TLC.
- Applications
• Blue/white cloning
systems.
• Immunoblotting and
immunocyto chemical assays.
• Microbiology media and
cell culture media.
- Formula
C14H15BrINO6
- Molecular Weight
908.6
- Storage Conditions
-20ºC, in a
constant-temperature
freezer.
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45