HybriPol DNA
Polymerase
AccuSure DNA Polymerase
AccuSure Mix
ACCUZYME DNA Polymerase,
high-fidelity
ACCUZYME Mix
BioMix Red, ready-to-go format
BioMix, ready-to-go format
BIOTAQ Core Kit
BIOTAQ
DNA Polymerase, regular applications, very robust
BIOTAQ RED DNA Polymerase, regular applications, very robust
BIO-X-ACT
Long DNA Polymerase, difficult applications
BIO-X-ACT Long Mix
BIO-X-ACT
Short DNA Polymerase, difficult applications
BIO-X-ACT Short Mix
Diamond DNA Polymerase,
high specificity
Diamond Mix
HybriPol DNA Polymerase
IMMOLASE DNA Polymerase
ImmoMix Red,
ready-to-go, heat-activated
ImmoMix, ready-to-go,
heat-activated
Klenow Fragment
MangoTaq DNA Polymerase
T4 DNA Polymerase,in-vitro
mutagenesis
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| PRODUCT |
SIZE
|
CONC.
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CAT NO.
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QUANTITY
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HybriPol DNA Polymerase |
2500u
|
5u/µl
|
BIO-21081
|
1
|
|
|
- Features
Active at a wide range of Mg
templates
Fusion protein with
different properties to Taq
- Description
HybriPol DNA Polymerase is a
novel thermostable DNA
polymerase created via the
fusion of two proteins.
HybriPol leaves an A’
overhang such that the
primer extension product is
suitable for effective
integration into TA cloning
vectors.
Specificity and performance
of HybriPol can be increased
with the use of 2x Poly-Mate
(not supplied, see
associated products), which
is designed for GC or AT
rich DNA, “dirty” templates
or sequences with difficult
melting profiles.
- Unit Definition
One unit will incorporate
10nmoles of dNTPs in 30min
at 72°C
- Storage Conditions
HybriPol can be stored at
–20°C in a constant
temperature freezer for
12months
- Notes
Purchase of this product
does not convey a license to
perform any patented
process.
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| PRODUCT |
SIZE
|
CONC.
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CAT NO.
|
QUANTITY
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AccuSure DNA
Polymerase |
250u
|
2.5u/µl
|
BIO-21068
|
1
|
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|
AccuSure DNA
Polymerase |
500u
|
2.5u/µl
|
BIO-21069
|
1
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|
- Features
-
- Extremely
high-fidelity.
-
Heat-activated.
- Very high
specificity.
- Fragments up
to 3Kb.
-
- Description
AccuSure is a
heat-activated
polymerase
possessing 5’ - 3’
and 3’ - 5’
proof-reading
activities, which
prevents
mis-incorporations
during primer
extension reactions.
AccuSure polymerises
regions of DNA such
as secondary
structures or
microsatellites,
which can be
problematic with
other polymerases.
In conjunction with
2x PolyMate,
AccuSure offers
enhanced specificity
in cases of
difficult melting
profiles.
- Unit
Definition
One unit is defined
as the amount of
enzyme that
incorporates
10nmoles of dNTPs
into acid-insoluble
form in 30min at
72°C.
- Storage
Conditions
AccuSure can be
stored for 12 months
at -20°C in a
constant-temperature
freezer
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
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| AccuSure Mix |
100 reactions
|
N/A
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BIO-25029
|
1
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| AccuSure Mix |
500 reactions
|
N/A
|
BIO-25030
|
1
|
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- Features
-
- Convenient ready-to-go format.
- Minimal handling, all
pre-optimised solutions.
- Coupled high-fidelity
and high specificity in a
single polymerase.
- Heat-activated.
- Description
AccuSure Mix is a 2x reagent
mix which combines great
convenience and high
performance in a single
product.
Since AccuSure Mix is
heat-activated, reactions
can be set up at room
temperature without the risk
of non-specific product
formation.
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72ºC.
- Storage Conditions
Long-term: 6 months at -20ºC
in a constant-temperature
freezer.
Short-term: 2 weeks at +4ºC
- Extended Stability
AccuSure Mix was stored at
+20ºC over a 5 week period
and tested daily. No
detectable loss of activity
was evidenced. However, due
to the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| ACCUZYME DNA
Polymerase, high-fidelity |
250u
|
2.5u/µl
|
BIO-21051
|
1
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| ACCUZYME DNA
Polymerase, high-fidelity |
500u
|
2.5u/µl
|
BIO-21052
|
1
|
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|
- Features
-
- ≥47-fold higher fidelity than Taq.
- Produces blunt ends.
- Processes up to 5Kb with
high-fidelity.
- Description
ACCUZYME is an enzyme
possessing 5’ - 3’ DNA
polymerase and 3’ - 5’
proof-reading exonuclease
activities, offering
extremely high-fidelity.
ACCUZYME is supplied with
10x buffer containing MgSO4
which provides optimal final
reaction conditions (2mM
Mg2+) for most experiments.
In order to allow
optimisation of reaction
conditions, additional MgCl2
is also provided.
When specificity and yield
of ACCUZYME DNA Polymerase
are essential, these can be
improved with the use of 2x
PolyMate (not supplied),
which is designed to amplify
GC- or AT-rich, “dirty”
templates or sequences with
a difficult melting profile.
ACCUZYME is recommended for
applications which require
extremely high-fidelity
and/or blunt ending.
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72°C
- Storage Conditions
ACCUZYME can be stored for
12 months at -20°C in a
constant-temperature
freezer.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
|
QUANTITY
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| ACCUZYME Mix |
100 reactions
|
N/A
|
BIO-25027
|
1
|
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| ACCUZYME Mix |
500 reactions
|
N/A
|
BIO-25028
|
1
|
|
|
- Features
-
- Convenient
pre-mixed, pre-optimised
solutions.
- Same high-fidelity
and specificity as
ACCUZYME.
- Suitable for
high-throughput
applications.
-
- Description
ACCUZYME Mix offers
high-fidelity and
high-specificity in a
convenient, ready-to-go, 2x
single polymerase mix.
Since ACCUZYME Mix is
pre-mixed, hands-on time is
dramatically reduced and the
scope for contamination is
lower.
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72ºC.
- Storage Conditions
Long-term: 6 months at -20ºC
in a constant-temperature
freezer.
Short-term: 2 weeks at +4ºC.
- Extended Stability
ACCUZYME Mix was stored at
+20ºC over a 5 week period
and tested daily. No
detectable loss of activity
was evidenced. However, due
to the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| BioMix Red,
ready-to-go format |
100 reactions
|
1.5mM
|
BIO-25005
|
1
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| BioMix Red,
ready-to-go format |
500 reactions
|
1.5mM
|
BIO-25006
|
1
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|
- Features
- Convenient ready-to-go mix coupled with red dye.
- Reproducible results.
- Reduced risk of
contamination.
- Pre-mixed, pre-optimised
solutions
- Description
BioMix Red is a ready-to-go
2x reaction mix containing
BIOTAQ Red DNA Polymerase,
to which the user adds only
water, template and primers.
BioMix Red also contains
MgCl2 (1.5mM) and ultra-pure
dNTPs manufactured by
Bioline, delivering
outstanding performance.
BioMix Red dramatically
reduces the time required to
set up reactions, thereby
reducing the risk of
contamination
- Storage Conditions
Long-term: 6 months at -20ºC
in a constant-temperature
freezer.
Short term: 2 weeks at +4°C
- Extended Stability
BioMix Red was stored at
+20ºC over a 5 week period
and tested daily. No
detectable loss of activity
was evidenced. However, due
to the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| BioMix, ready-to-go
format |
100 reactions
|
1.5mM
|
BIO-25011
|
1
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| BioMix, ready-to-go
format |
500 reactions
|
1.5mM
|
BIO-25012
|
1
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- Features
-
- Same high performance as BIOTAQ
- Convenient, ready-to-go.
- Reduced risk of
contamination.
- Reproducible results.
- Pre-mixed, pre-optimised
solutions.
- Description
BioMix is a ready-to-go 2x
reaction mix containing
BIOTAQ DNA Polymerase, to
which the user adds only
water, template and primers.
BioMix also contains MgCl2
(1.5mM) and ultra-pure dNTPs
manufactured by Bioline,
delivering outstanding
performance. BioMix
dramatically reduces the
time required to set up
reactions, thereby reducing
the risk of contamination
- Storage Conditions
Long-term: 6 months at -20ºC
in a constant- temperature
freezer.
Short term: 2 weeks at +4°C
- Extended Stability
BioMix was stored at +20ºC
over a 5 week period and
tested daily. No detectable
loss of activity was
evidenced. However, due to
the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| BIOTAQ Core Kit |
500u
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N/A
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BIO-21071
|
1
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- Features
-
- Complete set of
individual optimised
components to perform
polymerase assays.
- Contains ultra-pure
dNTPs manufactured by
Bioline.
- Ideal for new labs
setting up new
protocols.
- Product Description
The BIOTAQ Core Kit contains
all the reagents necessary
to perform polymerase assays
on a wide range of DNA
templates. The Core Kit
contains our widely used
BIOTAQ which offers
dependable high-performance
in routine or
high-throughput assays.
- Components
-
- 500 Units of BIOTAQ
DNA Polymerase
- 2 x 1ml 10x NH4
Buffer (No Mg2+)
- 1ml 50mM MgCl2
Solution
- 1ml 40mM dNTP Mix
1ml 2x PolyMate Additive
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72°C.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| BIOTAQ DNA Polymerase,
regular applications, very robust |
500u
|
5u/µl
|
BIO-21040
|
1
|
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| BIOTAQ DNA Polymerase,
regular applications, very robust |
2500u
|
5u/µl
|
BIO-21060
|
1
|
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|
- Features
• Robust performance in a
wide range of applications.- • Leaves ’A’ overhang.
- • Excellent
performance/price.
- • Separate MgCl2
solution supplied.
- Description
BIOTAQ DNA Polymerase is a
highly purified thermostable
DNA polymerase offering very
high yield over a wide range
of templates and is the
ideal choice for most
assays. BIOTAQ is a robust
preparation and consistently
delivers high yields with
minimal background. BIOTAQ
possesses 5’-3’ exonuclease
activity.
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72°C.
- Storage Conditions
BIOTAQ can be stored for 12
months at -20°C in a
constant-temperature
freezer.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| BIOTAQ RED DNA
Polymerase, regular applications, very
robust |
500u
|
1u/µl
|
BIO-21041
|
1
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| BIOTAQ RED DNA
Polymerase, regular applications, very
robust |
2500u
|
1u/µl
|
BIO-21061
|
1
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|
- Features
• The same high performance
as BIOTAQ DNA Polymerase.
- • Direct loading onto
agarose gels.
• Highly suited to
high-throughput
applications.
• Easy visual recognition.
• Separate MgCl2
solution supplied.
- • Leaves ’A’ overhang.
- Description
BIOTAQ Red DNA Polymerase
contains our widely used
BIOTAQ, and an inert red
dye.
BIOTAQ Red is an ideal
choice for high-throughput
applications, since it
combines a lower Taq
concentration with a dye to
facilitate easy recognition.
When the reaction is
complete, a sample of the
reaction mix can be loaded
directly onto agarose gels,
without the need for loading
buffer, since the sample
will be sufficiently dense
to sink to the bottom of the
gel unassisted.
BIOTAQ Red possesses 5’-3’
exonuclease activity
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72°C.
- Storage Conditions
BIOTAQ Red can be stored for
12 months at -20°C in a
constant-temperature
freezer.
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| PRODUCT |
SIZE
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CONC.
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CAT NO.
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QUANTITY
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| BIO-X-ACT Long DNA
Polymerase, difficult applications |
250u
|
4u/µl
|
BIO-21049
|
1
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| BIO-X-ACT Long DNA
Polymerase, difficult applications |
500u
|
4u/µl
|
BIO-21050
|
1
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|
- Product Description
- BIO-X-ACT DNA
Polymerases are a
high-performance proprietary
complex of enzymes and
additives specifically
designed for difficult or
problematic applications
requiring high processivity
with high-fidelity. The
unique composition of
different enzymatic
activities delivers a
17-fold increase in fidelity
in comparison with regular
Taq polymerase and is
therefore suitable for
reactions where accuracy is
important. Two versions of
BIO-X-ACT are available,
depending on the length of
the anticipated fragment:
BIO-X-ACT Long DNA
Polymerase and
BIO-X-ACT Short DNA
Polymerase
BIO-X-ACT Long DNA
Polymerase is recommended
for longer Genomic DNA
fragments of between 2Kb and
20Kb, or 30Kb Lambda DNA
fragments. With Lambda DNA
as the template, the best
performance is achieved in
the 2-20Kb range. BIO-X-ACT
long is our original widely
used BIO-X-ACT formulation
BIO-X-ACT possesses 5'-3'
DNA polymerase activity and
3'-5' proof-reading activity
which prevents
mis-incorporations of
nucleotides during a
reaction.
BIO-X-ACT is supplied with
its own unique buffer and
also with "HiSpec Additive",
which reduces smears,
primer-dimers and spurious
bands that are associated
with difficult GC-rich and
repetitive sequences. The
Additive is included with
all pack sizes of BIO-X-ACT.
- Features
- • Suited to difficult
templates
• High processivity, high
performance
• Long-range applications
• >17-fold higher fidelity
than Taq
• Leaves 'A' overhang
• Eliminates non-specific
artefacts
- Applications
- Long-Region
Applications:
Optimal composition of
different enzymatic
activities enables BIO-X-ACT
Long to span the primer
extension over long regions
and demonstrate high
processivity by reducing
premature strand termination
and template degradation.
Using long primers at
elevated Mg++
concentrations, >30Kb or
20Kb products can be
achieved from Lambda
templates or genomic DNA,
respectively.
General BIO-X-ACT
Applications:
• High Performance:
BIO-X-ACT provides a high
yield and specificity, even
with 'dirty' DNA or
difficult templates with an
unfavourable nucleotide
composition. In contrast to
standard 3'-5' proof-reading
polymerases, BIO-X-ACT can
be used in combination with
degenerate or non-perfect
matching primers.
• Direct Cloning: BIO-X-ACT
is recommended for direct
gene cloning without the
need to verify the sequence
prior to expression.
BIO-X-ACT leaves an ‘A’
overhang such that the
extended products are
suitable for effective
integration into TA cloning
vectors, even from difficult
templates.
• High Fidelity: BIO-X-ACT
is a mix of polymerases that
possesses a 5'-3' DNA
polymerase activity and
3'-5' proof- reading
activity which reduces
misincorporations during
primer extension. This
combination of properties
provides a >17 fold higher
fidelity than Taq. In
contrast with other
proof-reading enzymes,
BIO-X-ACT does not degrade
primers.
• High Specificity:
BIO-X-ACT is supplied with a
vial of a unique specificity
enhancer. 5x Hi-Spec
Additive helps to prevent
the formation of false
background bands and
smearing, especially on
difficult templates. Hi-Spec
Additive should be used at
1.0-2.0x final concentration
- the optimal amount
required should be
determined for each
individual experiment.
Hi-Spec Additive may also
alter the ideal annealing
temperature for primers -
some optimization may be
required.
Specificity and yield of the
BIO-X-ACT Polymerases can be
increased with the use of
2x PolyMate Additive
which is the improved
version of Hi-Spec Additive
designed for cases of GC-,
AT- rich, ‘dirty’ templates,
or sequences with a
difficult melting profile
when yield and specificity
are in demand.
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
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| BIO-X-ACT Long Mix |
100 reactions
|
N/A
|
BIO-25023
|
1
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| BIO-X-ACT Long Mix |
500 reactions
|
N/A
|
BIO-25024
|
1
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- Features
-
- A proprietary
reaction mix,
ready-to-go for
problematic templates.
- Minimal handling.
- Pre-mixed,
pre-optimised solution.
- Up to 20Kb genomic
templates.
-
- Description
A ready-to-go mix for
problematic templates
requiring high-fidelity ≤
20Kb. BIO-X-ACT Long Mix is
the ideal choice for the
user who has problematic
templates and wishes to
minimise handling or the
possibility of
contamination.
- Storage Conditions
Long-term: 6 months at -20°C
in a constant-temperature
freezer.
Short-term: 2 weeks at +4°C
- Extended Stability
BIO-X-ACT Long Mix was
stored at +20ºC over a 5
week period and tested
daily. No detectable loss of
activity was evidenced.
However, due to the
potential of microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
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| BIO-X-ACT Short DNA
Polymerase, difficult applications |
250u
|
4u/µl
|
BIO-21064
|
1
|
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| BIO-X-ACT Short DNA
Polymerase, difficult applications |
500u
|
4u/µl
|
BIO-21065
|
1
|
|
|
- Product Description
- BIO-X-ACT DNA
Polymerases are a
high-performance proprietary
complex of enzymes and
additives specifically
designed for difficult or
problematic applications
requiring high processivity
with high-fidelity. The
unique composition of
different enzymatic
activities delivers a
17-fold increase in fidelity
in comparison with regular
Taq polymerase and is
therefore suitable for
reactions where accuracy is
important. Two versions of
BIO-X-ACT are available,
depending on the length of
the anticipated fragment:
BIO-X-ACT Short DNA
Polymerase and
BIO-X-ACT Long DNA
Polymerase
BIO-X-ACT Short DNA
Polymerase is recommended
for short Genomic DNA
fragments of up to 2Kb, or
up to 5Kb on Lambda DNA.
With Lambda DNA as the
template, the best
performance is achieved
within the 100bp to 3Kb
range.
BIO-X-ACT possesses 5'-3'
DNA polymerase activity and
3'-5' proof-reading activity
which prevents
mis-incorporations of
nucleotides during a
reaction.
BIO-X-ACT is supplied with
its own unique buffer and
also with "HiSpec Additive",
which reduces smears,
primer-dimers and spurious
bands that are associated
with difficult GC-rich and
repetitive sequences. The
Additive is included with
all pack sizes of BIO-X-ACT.
- Features
- • Suited to difficult
templates
• High processivity, high
performance
• >17-fold higher fidelity
than Taq
• Leaves 'A' overhang
• Eliminates non-specific
artefacts
- Applications
- Applications for
BIO-X-ACT Short:
BIO-X-ACT Short is a newer
member of the BIO-X-ACT
family of polymerases, and
is designed specifically for
short-region applications of
<2Kb.
General BIO-X-ACT
Applications:
• High Performance:
BIO-X-ACT provides a high
yield and specificity, even
with 'dirty' DNA or
difficult templates with an
unfavourable nucleotide
composition. In contrast to
standard 3'-5' proof-reading
polymerases, BIO-X-ACT can
be used in combination with
degenerate or non-perfect
matching primers.
• Direct Cloning: BIO-X-ACT
is recommended for direct
gene cloning without the
need to verify the sequence
prior to expression.
BIO-X-ACT leaves an ‘A’
overhang such that the
extended products are
suitable for effective
integration into TA cloning
vectors, even from difficult
templates.
• High Fidelity: BIO-X-ACT
is a mix of polymerases that
possesses a 5'-3' DNA
polymerase activity and
3'-5' proof- reading
activity which reduces
misincorporations during
primer extension. This
combination of properties
provides a >17 fold higher
fidelity than Taq. In
contrast with other
proof-reading enzymes,
BIO-X-ACT does not degrade
primers.
• High Specificity:
BIO-X-ACT is supplied with a
vial of a unique specificity
enhancer. 5x Hi-Spec
Additive helps to prevent
the formation of false
background bands and
smearing, especially on
difficult templates. Hi-Spec
Additive should be used at
1.0-2.0x final concentration
- the optimal amount
required should be
determined for each
individual experiment.
Hi-Spec Additive may also
alter the ideal annealing
temperature for primers -
some optimization may be
required.
Specificity and yield of the
BIO-X-ACT Polymerases can be
increased with the use of
2x Poly-Mate Additive
which is the improved
version of Hi-Spec Additive
designed for cases of GC-,
AT- rich, ‘dirty’ templates,
or sequences with a
difficult melting profile
when yield and specificity
are in demand.
|
|
|
|
|
|
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| BIO-X-ACT Short Mix |
100 reactions
|
N/A
|
BIO-21025
|
1
|
|
|
| BIO-X-ACT Short Mix |
500 reactions
|
N/A
|
BIO-21026
|
1
|
|
|
- Features
-
- A proprietary
ready-to-go enzyme mix,
for problematic
templates.
- Minimal handling.
- Pre-mixed,
pre-optimised solution.
- Suited to short
genomic templates up to
3Kb.
- Description
A ready-to-go mix for
problematic templates
requiring high-fidelity ≤3
Kb.
BIO-X-ACT Short Mix is the
ideal choice for the user
who has a problematic
template and wishes to
minimise handling or the
possibility of
contamination.
BIO-X-ACT is available in
two versions for short and
long templates respectively.
- Storage Conditions
Long-term: 6 months at -20°C
in a constant-temperature
freezer.
Short-term: 2 weeks at +4°C.
- Extended Stability
BIO-X-ACT Short Mix was
stored at +20ºC over a 5
week period and tested
daily. No detectable loss of
activity was evidenced.
However, due to the
potential of microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided.
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Diamond DNA
Polymerase, high specificity |
250u
|
5u/µl
|
BIO-21058
|
1
|
|
|
| Diamond DNA
Polymerase, high specificity |
500u
|
5u/µl
|
BIO-21059
|
1
|
|
|
- Product Description
- Diamond DNA Polymerase
offers unrivalled
specificity and is designed
to cope with difficult
templates, such as high GC%
regions and microsatellites.
Its unique active site
confirmation means that only
correctly matched bases can
be extended, virtually
eliminating the possibility
of any base substitutions in
the reaction product. It is
ideal for applications where
specificity is crucial and
is particularly well suited
to primer extension of
Single Nucleotide
Polymorphism (SNP) markers.
Diamond DNA Polymerase is
capable of extending through
regions that other
polymerases find difficult,
such as inverted tandem
repeats and those with high
amounts of secondary
structure. Tests have
demonstrated that BIOLASE
Diamond has the lowest
signal/noise ratio and
almost no ‘stutter’ when
compared with other
commercially available
enzymes.
- Features
- • Very low signal/noise
ratio
• High Specificity
• High Sensitivity
- Applications
- • Multiplex, SNP
detection, genotyping,
secondary structures
• Suitable for applications
possessing GC-rich regions
or repeats, such as
microsatellites
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Diamond Mix |
100 reactions
|
N/A
|
BIO-25031
|
1
|
|
|
| Diamond Mix |
500 reactions
|
N/A
|
BIO-25032
|
1
|
|
|
- Features
-
- The same performance
as Diamond, but in a
convenient ready-to-go
format.
- Extremely low
signal/noise ratio.
- Description
Extremely high specificity
in a ready-to-go mix.
Diamond Mix is a ready-to-go
2x product for users seeking
extremely high specificity
in a range of difficult
templates. Diamond Mix is
highly suited to SNP
detection.
- Storage Conditions
Long-term: 6 months at -20°C
in a constant-temperature
freezer.
Short-term: 2 weeks at +4°C
- Extended Stability
Diamond Mix was stored at
+20ºC over a 5 week period
and tested daily. No
detectable loss of activity
was evidenced. However, due
to the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided.
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| HybriPol DNA
Polymerase |
1000u
|
5u/µl
|
BIO-21080
|
1
|
|
|
- Features
Active at a wide range of Mg
templates
Fusion protein with
different properties to Taq
- Description
HybriPol DNA Polymerase is a
novel thermostable DNA
polymerase created via the
fusion of two proteins.
HybriPol leaves an A’
overhang such that the
primer extension product is
suitable for effective
integration into TA cloning
vectors.
Specificity and performance
of HybriPol can be increased
with the use of 2x Poly-Mate
(not supplied, see
associated products), which
is designed for GC or AT
rich DNA, “dirty” templates
or sequences with difficult
melting profiles.
- Unit Definition
One unit will incorporate
10nmoles of dNTPs in 30min
at 72°C
- Storage Conditions
HybriPol can be stored at
–20°C in a constant
temperature freezer for
12months
- Notes
Purchase of this product
does not convey a license to
perform any patented
process.
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| IMMOLASE DNA
Polymerase |
250u
|
5u/µl
|
BIO-21046
|
1
|
|
|
| IMMOLASE DNA
Polymerase |
500u
|
5u/µl
|
BIO-21047
|
1
|
|
|
| IMMOLASE DNA
Polymerase |
5000u
|
5u/µl
|
BIO-21048
|
1
|
|
|
- Features
• Heat-activated, inactive
at room temperature.
• Ultra high specificity.
• Eliminates non-specific
reaction products.
• Leaves ’A’ overhang.
- Description
IMMOLASE is a heat-activated
form of a thermostable DNA
polymerase isolated from a
novel organism. IMMOLASE
provides improved
specificity when compared to
standard DNA polymerases and
can eliminate the presence
of non-specifics, such as
primer-dimers and mis-primed
products. IMMOLASE is
inactive at room temperature
and must therefore be
activated by heat treatment
before primer extension.
Subsequently, the reaction
can be handled according to
the user’s existing
protocols for thermostable
DNA polymerases.
- Unit Definition
One unit is defined as the
amount of enzyme that
incorporates 10nmoles of
dNTPs into acid-insoluble
form in 30min at 72°C.
- Storage Conditions
IMMOLASE can be stored for
12 months at -20°C in a
constant-temperature freezer
- Figure 1:
-
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
|
| ImmoMix Red,
ready-to-go, heat-activated |
100 reactions
|
1.5mM
|
BIO-25021
|
1
|
|
|
|
ImmoMix Red,
ready-to-go, heat-activated |
500 reactions
|
1.5mM
|
BIO-25022
|
1
|
|
|
- Features
• Contains red dye for easy
visualisation.
• The same strong
performance as ImmoMix.
• Suited to high-throughput
applications.
- Description
ImmoMix Red is a complete
ready-to-go heat-activated
2x reaction mix, which only
requires the user to add
water, template and primers
and then to pre-heat to
95°C. The mix is composed
of: IMMOLASE DNA Polymerase,
dNTPs, magnesium chloride
(1.5mM) and a non-toxic,
non-hazardous red dye,
allowing users to ensure
adequate mixing. ImmoMix Red
dramatically reduces the
amount of time needed to set
up reactions, thereby
reducing the risk of
contamination. The number of
pipetting steps is also
minimised, ensuring greater
reproducibility. Once the
reactions are completed,
they can be loaded directly
onto a gel, without the need
to add loading buffer
- Storage Conditions
Long-term: 6 months at -20°C
in a constant-temperature
freezer.
Short-term: 2 weeks at +4°C
- Extended Stability
ImmoMix Red was stored at
+20ºC over a 5 week period
and tested daily. No
detectable loss of activity
was evidenced. However, due
to the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| ImmoMix, ready-to-go,
heat-activated |
100 reactions
|
1.5mM
|
BIO-25019
|
1
|
|
|
| ImmoMix, ready-to-go,
heat-activated |
500 reactions
|
1.5mM
|
BIO-25020
|
1
|
|
|
- Features
• Heat-activated.
• Ultra high specificity.
• Pre-mixed, pre-optimised
solutions.
• Leaves ’A’ overhang.
- Description
ImmoMix is a complete
ready-to-go heat-activated
2x reaction mix, which only
requires the user to add
water, template and primers
and then to pre-heat to
95°C. The mix is composed
of: IMMOLASE DNA Polymerase,
dNTPs and magnesium chloride
(1.5mM). ImmoMix
dramatically reduces the
amount of time needed to set
up reactions, thereby
reducing the risk of
contamination. The number of
pipetting steps is also
minimised, ensuring greater
reproducibility
- Storage Conditions
Long-term: 6 months at -20°C
in a constant-temperature
freezer.
Short-term: 2 weeks at +4°C
- Extended Stability
ImmoMix was stored at +20ºC
over a 5 week period and
tested daily. No detectable
loss of activity was
evidenced. However, due to
the potential of
microbiological
contamination, please adhere
to the storage conditions
outlined above.
Repeated freeze/thaw should
be avoided.
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Klenow Fragment
|
500u
|
5u/µl
|
BIO-27029
|
1
|
|
|
- Features
• Lacks any exonuclease
activity.
• Sequencing grade.
- Description
Klenow fragment is a
proteolytic subfragment of
E.coli DNA polymerase I,
obtained by subtilising
cleavage of the whole
enzyme. The enzyme is
purified from E.coli PVG-AI
strain.
- Applications
• Synthesis of the second
strand of cDNA.
• Radiolabelling by filling
in 5’ single-stranded
extensions on
double-stranded DNA.
• Generation of blunt-ended
DNA fragments.
• Dideoxy sequencing.
- Storage Conditions
Klenow Fragment can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
|
|
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| MangoTaq DNA
Polymerase |
1000u
|
1u/µl
|
BIO-21083
|
1
|
|
|
| MangoTaq DNA
Polymerase |
2000u
|
1u/µl
|
BIO-21082
|
1
|
|
|
| MangoTaq DNA
Polymerase |
5000u
|
1u/µl
|
BIO-21078
|
1
|
|
|
- Description
MangoTaq
DNA Polymerase, from Bioline
is a new formulation of
Taq DNA Polymerase, is
supplied with a coloured 10x
Reaction Buffer which
contains two inert dyes. The
Red and Orange Dyes separate
during gel electrophoresis
and provide quick reference
points to monitor mobility
of DNA samples in the gel.
The reaction mixture
containing the inert
Red/Orange dyes can be
loaded directly onto an
agarose gel for analysis,
without the need for
separate gel-loading buffer.
MangoTaq
offers consistent results
across a wide range of DNA
templates and also leaves an
‘A’ overhang such that the
primer-extension product is
suitable for effective
integration into TA cloning
vectors. MangoTaq
is an ideal choice for
high-throughput
applications, since it
combines a lower Taq
concentration (1u/μl) with
inert colored dyes to
facilitate easy recognition.
- Features
-
- Robust performance
in a wide range of
applications
- 10x reaction buffer
contains inert red and
orange dyes which allow
direct gel-loading
- Leaves ‘A’ overhang
- Excellent
price/performance
- Highly suited to
high-throughput
applications
- Separate MgCl2supplied
|
TBE
Buffer (5, 10, 15
and 20 μl of
amplified reactions,
in wells
prior to
electrophoresis)
|
 |
Red and orange
dyes after
electrophoresis.
Above amplification
reactions subjected
to electrophoresis. |
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| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| T4 DNA
Polymerase,in-vitro mutagenesis |
250u
|
|
BIO-27034
|
1
|
|
|
| T4 DNA
Polymerase,in-vitro mutagenesis |
500u
|
|
BIO-27035
|
1
|
|
|
- Features
• Possesses 5’-3’ DNA
polymerase and 3’-5’
proof-reading exonuclease
activity.
• Lacks 5’-3’ exonuclease
activity.
- Description
T4 DNA Polymerase catalyses
the synthesis of DNA in the
5’-3’ direction in the
presence of template and
primers. This enzyme has
3’-5’ proof-reading
exonuclease activity.
- Applications
• In vitro mutagenesis.
• Probe-labelling.
• Removal of 3’ overhang to
form blunt ends
- Storage Conditions
T4 DNA Polymerase can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
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+32 (0) 16 58 90 45
+32 (0) 16 50 90 45