Nucleotides
ACGU + T Mix ( 50x Solution)
dATP
dCTP
dGTP
dITP
dNTP Mix
dNTP Set
dTTP
dUTP
dUTP Mix
NTP Mix (100µmol)
NTP Set (4x25µmol)
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| ACGU + T Mix ( 50x
Solution) |
20 µmol
|
40mM
|
BIO-39045
|
1
|
|
|
- Features
-
- Prevents carry-over
of amplified products
into subsequent
amplifications.
- PCR grade.
- Convenient
pre-optimised, premixed
solution.
- ACGU & T mix
contains dATP, dCTP,
dGTP, dUTP and dTTP.
- Product Description
Bioline ACGU + T mix enables
users to employ the
efficient strategy of
incorporating dUTP into
amplification reactions.
By doing so, dUTP replaces
dTTP in amplified products.
Before a subsequent DNA
amplification is commenced,
treatment with Uracil DNA
Glycosylase (UDGase) will
result in the selective
cleavage of the Uracil base
in the dUTP-containing DNA,
therefore preventing
carry-over contaminations.
- Applications
-
- Storage Conditions
ACGU + T mix can be stored
for 12 months at -20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage, aliquoting
is recommended.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dATP
|
25µmol
|
100mM as 1 x 250µl
|
BIO-39036
|
1
|
|
|
- Features
• Ultra-pure: >99%
triphosphate by HPLC.
• Enhanced storage life.
• Convenient solutions at pH
7.5.
- Description
Ready-to-use molecular-grade
individual dNTP solutions
for use in DNA
polymerisation reactions,
DNA labelling and sequencing
processes
- Applications
PCR, Long PCR, Real-Time
PCR, dideoxy sequencing,
mutagenesis and cDNA
sequencing.
- High dNTP purity for
long range PCR
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Quality Control Tests
Quality control by
HPLC for maximum purity
and minimal di- and
mono-phosphate content.
All batches are
tested in standard
labelling reactions.
Storage
Conditions
Bioline dNTPs can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage,
aliquoting is
recommended
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
 max
pH 7.5 |
259nm |
272nm |
252nm |
 at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dCTP |
25µmol
|
100mM as 1 x 250µl
|
BIO-39038
|
1
|
|
|
- Features
• Ultra-pure: >99%
triphosphate by HPLC.
• Enhanced storage life.
• Convenient solutions at pH
7.5.
- Description
Ready-to-use molecular-grade
individual dNTP solutions
for use in DNA
polymerisation reactions,
DNA labelling and sequencing
processes
- Applications
PCR, Long PCR, Real-Time
PCR, dideoxy sequencing,
mutagenesis and cDNA
sequencing.
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Quality Control Tests
Quality control by
HPLC for maximum purity
and minimal di- and
mono-phosphate content.
All batches are
tested in standard
labelling reactions.
Storage
Conditions
Bioline dNTPs can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage,
aliquoting is
recommended
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dGTP |
25µmol
|
100mM as 1 x 250µl
|
BIO-39037
|
1
|
|
|
- Features
• Ultra-pure: >99%
triphosphate by HPLC.
• Enhanced storage life.
• Convenient solutions at pH
7.5.
- Description
Ready-to-use molecular-grade
individual dNTP solutions
for use in DNA
polymerisation reactions,
DNA labelling and sequencing
processes
- Applications
PCR, Long PCR, Real-Time
PCR, dideoxy sequencing,
mutagenesis and cDNA
sequencing.
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Quality Control Tests
Quality control by
HPLC for maximum purity
and minimal di- and
mono-phosphate content.
All batches are
tested in standard
labelling reactions.
Storage
Conditions
Bioline dNTPs can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage,
aliquoting is
recommended
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dITP |
25µmol
|
100mM
|
BIO-39032
|
1
|
|
|
- Features
• Ultra-pure: >99%
triphosphate by HPLC.
• Enhanced storage life.
• Convenient solutions at pH
7.5.
- Description
Ready-to-use molecular-grade
individual dNTP solutions
for use in DNA
polymerisation reactions,
DNA labelling and sequencing
processes
- Applications
PCR, Long PCR, Real-Time
PCR, dideoxy sequencing,
mutagenesis and cDNA
sequencing.
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Quality Control Tests
Quality control by
HPLC for maximum purity
and minimal di- and
mono-phosphate content.
All batches are
tested in standard
labelling reactions.
Storage
Conditions
Bioline dNTPs can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage,
aliquoting is
recommended
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dNTP Mix
|
10µmol (1ml vol.)
|
10 mM
|
BIO-39044
|
1
|
|
|
| dNTP Mix |
4 x 5µmol
|
40mM
|
BIO-39043
|
1
|
|
|
| dNTP Mix
|
50µmol dNTP
|
100mM as 1 x 500µl
|
BIO-39028
|
1
|
|
|
| dNTP Mix
|
200µmol dNTP
|
100mM as 4 x 500µl
|
BIO-39029
|
1
|
|
|
| dNTP Mix
|
100µmol (10x1ml)
|
10 mM
|
BIO-39053
|
1
|
|
|
| dNTP Mix
|
100µmol (20x1ml)
|
10 mM
|
BIO-39054
|
1
|
|
|
- Product Description
- A ready-to-use
molecular-grade dNTP mix
containing four dNTPs. The
mix is designed to save
hands-on time for
researchers and reduces the
possibility of contamination
by reducing pipetting.
- Features
- • High purity: >99% by
HPLC
• Convenient solution at pH
7.5
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide. |
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dNTP Set |
4 x 25µmol
|
100mM
|
BIO-39025
|
1
|
|
|
| dNTP Set |
4 x 100µmol
|
100mM
|
BIO-39026
|
1
|
|
|
| dNTP Set |
4 x 500µmol
|
100mM
|
BIO-39027
|
1
|
|
|
- Product Description
Ready-to-use molecular-grade
individual dNTP solutions,
supplied as sets (dATP,
dCTP, dGTP, dTTP) for use in
DNA polymerisation
reactions, DNA labelling and
sequencing processes.
- Features
• High purity: >99% by HPLC
• Convenient solution at pH
7.5
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dTTP |
25µmol
|
100mM as 1 x 250µl
|
BIO-39039
|
1
|
|
|
- Features
• Ultra-pure: >99%
triphosphate by HPLC.
• Enhanced storage life.
• Convenient solutions at pH
7.5.
- Description
Ready-to-use molecular-grade
individual dNTP solutions
for use in DNA
polymerisation reactions,
DNA labelling and sequencing
processes
- Applications
PCR, Long PCR, Real-Time
PCR, dideoxy sequencing,
mutagenesis and cDNA
sequencing.
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Quality Control Tests
Quality control by
HPLC for maximum purity
and minimal di- and
mono-phosphate content.
All batches are
tested in standard
labelling reactions.
Storage
Conditions
Bioline dNTPs can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage,
aliquoting is
recommended
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dUTP |
25µmol
|
100mM as 1 x 250µl
|
BIO-39035
|
1
|
|
|
- Features
• Ultra-pure: >99%
triphosphate by HPLC.
• Enhanced storage life.
• Convenient solutions at pH
7.5.
- Description
Ready-to-use molecular-grade
individual dNTP solutions
for use in DNA
polymerisation reactions,
DNA labelling and sequencing
processes
- Applications
PCR, Long PCR, Real-Time
PCR, dideoxy sequencing,
mutagenesis and cDNA
sequencing.
- High dNTP purity for
long range PCR
|
|
|
Lane 1:
HyperLadder I (top band
= 10 kb)
Lane 2: Amplification of
a 15 kb DNA fragment
Lane 3: Amplification of
a 20 kb DNA fragment
Impurities in PCR
reagents can often
inhibit PCR reactions.
This inhibition is often
observed in Long range
PCR reactions.
To demonstrate the
purity of Bioline’s
dNTPs, a long distance
20kb PCR was performed
as a stringent
(functional) test for
reagent quality and
visualized by
electrophoresis on a 1%
agarose gel in 1x TAE
stained with ethidium
bromide.
|
Quality Control Tests
Quality control by
HPLC for maximum purity
and minimal di- and
mono-phosphate content.
All batches are
tested in standard
labelling reactions.
Storage
Conditions
Bioline dNTPs can be
stored for 12 months at
-20°C in a
constant-temperature
freezer.
Avoid multiple
freeze-thawing. For
long-term usage,
aliquoting is
recommended
|
Physical constants, spectral and HPLC analysis
| |
dATP-Li4
|
dCTP-Li4
|
dGTP-Li4
|
|
Formula |
C10H12N5O12P3Li4 |
C10H12N3O13P3Li4 |
C10H12N5O13P3Li4 |
|
Molecular weight |
514.916g/mol |
490.891g/mol |
530.916g/mol |
|
max
pH 7.5 |
259nm |
272nm |
252nm |
|
at
max |
15.4 E x mmol
-1
x cm-1 |
9.1 E x mmol-1
x cm-1 |
13.7 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.80 ± 0.03 |
0.82 ± 0.03 |
1.15 ± 0.05 |
|
A 280
/A 260 |
0.15 ± 0.02 |
0.98 ± 0.05 |
0.67 ± 0.03 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>99% |
>99%
|
| |
dTTP-Li4
|
dITP-Li4
|
dUTP-Li4
|
|
Formula |
C10H13N2O14P3Li4 |
C10H12N4O13P3Li4 |
C9H12N2O14P3Li4 |
|
Molecular weight |
505.903g/mol |
516.909g/mol |
492.884g/mol |
|
max
pH 7.5 |
267nm |
249nm |
262nm |
|
at
max |
9.6 E x mmol-1
x cm-1 |
12.2 E x mmol
-1
x cm-1 |
10.0 E x mmol
-1
x cm-1 |
|
A 250
/A 260 |
0.65 ± 0.03 |
1.68 ± 0.04 |
0.75 ± 0.03 |
|
A 280
/A 260 |
0.70 ± 0.02 |
0.25 ± 0.02 |
0.38 ± 0.02 |
|
Concentration |
100mM ±2% |
100mM ±2% |
100mM ±2% |
|
pH of solution |
7.50 |
7.50 |
7.50 |
|
Purity (HPLC area) |
>99% |
>98% |
>98% |
HPLC Profile of >99% pure
dNTPs
dATP
dGTP
dCTP
dTTP
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| dUTP Mix |
50x Sol.
|
N/A
|
BIO-39041
|
1
|
|
|
- Features
-
- PCR grade.
- Conveniently
pre-optimised, premixed
solutions.
- Description
dUTP Mix is a 50x solution
which contains 10mM each of
dA, dC, dG and 20mM dUTP.
- Applications
-
- Storage Conditions
dUTP Mix can be stored for
12 months at -20C in a
constant-temperature
freezer. Avoid multiple
freeze-thawing. For
long-term usage, aliquoting
is recommended.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
|
NTP Mix
(100µmol) |
1ml
|
100mM
|
BIO-39050
|
1
|
|
|
- Features
-
- Suitable for in
vitro transcription.
- 98% pure by HPLC.
- Supplied as 100mM
solutions.
- Description
Bioline NTPs
(Ribonucleoside-5’
tri-phosphates) are
manufactured by Bioline and
are tested in functional
assays with Bioline’s RNA
polymerases.
- Applications
-
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| NTP Set (4x25µmol) |
4 x 250µl
|
100mM
|
BIO-39052
|
1
|
|
|
- Features
-
- Suitable for in
vitro transcription.
- 98% pure by HPLC.
- Supplied as 100mM
solutions.
- Description
Bioline NTPs
(Ribonucleoside-5’
tri-phosphates) are
manufactured by Bioline and
are tested in functional
assays with Bioline’s RNA
polymerases.
- Applications
-
|
|
|
|
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45