Additives and Buffers
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 10x AccuBuffer |
3 x 1.2ml
|
|
BIO-37033
|
1
|
|
|
- Product Description
- Concentration:600mM
Tris-Cl, 60mM (NH4)2SO4,
100mM KCl,20mM MgSO4,
pH 8.3 at 25°C
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 10x ImmoBuffer |
3 x 1.2ml
|
N/A
|
BIO-37044
|
1
|
|
|
- Composition
160mM (NH4)2SO4. 1M Tris-HCI
pH 8.3, 0.1% Tween-20.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 10x KCI + MgCl2 Buffer |
3 x 1.2ml
|
N/A
|
BIO-37066
|
1
|
|
|
- Composition
500mM KCI, 100mM Tris-CI (pH
8.8 at 25°C), 15mM MgCl2, 1%
Triton X-100 15mM.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 10x NH4 + MgCl2 Buffer |
3 x 1.2ml
|
N/A
|
BIO-37064
|
1
|
|
|
- Composition
160mM (NH4)2SO4, 670mM
Tris-HCI (pH8.8 at 25°C),
0.1% Tween-20, 15mM MgCl2.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer A |
2.5ml
|
N/A
|
BIO-37052
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer A
Buffer A contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer B |
2.5ml
|
N/A
|
BIO-37053
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer B
Buffer B contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer C |
2.5ml
|
N/A
|
BIO-37054
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer C
Buffer C contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer D |
2.5ml
|
N/A
|
BIO-37055
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer D
Buffer D contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer E |
2.5ml
|
N/A
|
BIO-37056
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer E
Buffer E contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer F |
2.5ml
|
N/A
|
BIO-37057
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer F
Buffer F contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer G |
2.5ml
|
N/A
|
BIO-37058
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer G
Buffer G contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer H |
2.5ml
|
N/A
|
BIO-37059
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer H
Buffer H contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer I |
2.5ml
|
N/A
|
BIO-37060
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer I
Buffer I contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer J |
2.5ml
|
N/A
|
BIO-37061
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer J
Buffer J contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer K |
2.5ml
|
N/A
|
BIO-37062
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer K
Buffer K contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x Buffer L |
2.5ml
|
N/A
|
BIO-37063
|
1
|
|
|
- Product Description
2X PCR Optimization
Buffer L
Buffer L contains Ammonium
Sulphate, Tricine, dNTPs,
variable concentration of
MgCl2(3-10mM),
and optimised composition of
additives - PCR enhancers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 2x PolyMate Additive |
2 x 1.2ml
|
|
BIO-37041
|
1
|
|
|
- Product Description
-
PolyMate is a
special 2x additive
for use in reactions
involving any
thermostable DNA
Polymerase. PolyMate
provides an
optimized
composition of
reagents, leading to
a dramatic
improvement of
reaction
specificity.
PolyMate is
recommended for use
with any
thermostable DNA
Polymerases.
By making DNA more
accessible for
Polymerase and
oligonucleotides,
PolyMate acts as a
“melting” agent for
dirty and mainly
difficult templates
with high GC- or AT-
content, and
repetitive
sequences, or
templates with a
difficult melting
profile.
PolyMate does not
contain Mg, dNTPs,
or buffer
components.
|
1 2 3
4
5 6 7
PCR of a
201bp G-C Rich
fragment >66% from
Human TGF-β gene
|
Lane 1-3: |
With
PolyMate
Additive &
1.5 mM,
2.0mM & 2.5
MgCl2
respectively |
|
Lane 4: |
HyperLadder
IV |
|
Lane 5-7: |
Without
PolyMate
Additive &
1.5 mM,
2.0mM & 2.5
MgCl2
respectively |
|
- Features
-
- Dramatically
increases specificity
- Compatible with any
thermostable DNA
Polymerases
- Ideal for
"difficult" templates
- Reduces smearing and
background
- Applications
-
- Universal additive
to all types of DNA
Polymerase reactions
- DNA Polymerase
reactions where
specificity is critical
- Storage Conditions
- PolyMate Additive can be
stored at +4°C (short-term)
and at -20°C, long-term.
- Notes
- PolyMate Additive should
not be used in combination
with other PCR Additives.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| 50mM MgSO4 |
3 x 1.2ml
|
N/A
|
BIO-37065
|
1
|
|
|
- Description
50mM MgSO4, Dnase and Rnase
free.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| BioEnhance |
~100 reactions
|
|
BIO-37028
|
1
|
|
|
| BioEnhance |
~300 reactions
|
|
BIO-37029
|
1
|
|
|
- Product Description
- BioEnhance improves any
enzyme reaction by
stabilizing the native
conformation of the enzyme
quaternary structure.
BioEnhance is compatible
with any pre-optimized
incubation buffer/additive
composition and also
increases enzyme
thermostability, which leads
to improved performance.
BioEnhance also prevents
biomolecules from premature
denaturation under thermal
or other stress conditions.
By fixing the native enzyme
conformation in DNA
polymerase reactions,
BioEnhance suppresses the
accumulation of false-primed
fragments, mismatch
extensions and concatamers.
- Features
- • Simple to use.
• Compatible with all
pre-optimized incubation
buffers.
- Applications
- • Enhances any enzyme
reaction by simple addition.
• Improves enzyme
thermostability and
half-life.
- Storage Conditions
- BioEnhance will remain
stable at –20°C in a
constant-temperature
freezer.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Coloured loading
buffer, Blue |
2x 1ml
|
N/A
|
BIO-37045
|
1
|
|
|
- Description
To monitor DNA migration.
5x DNA coloured loading
buffers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Coloured loading
buffer, Orange |
2x 1ml
|
N/A
|
BIO-37069
|
1
|
|
|
- Description
To monitor DNA migration.
5x DNA coloured loading
buffers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Coloured loading
buffer, Red |
2x 1ml
|
N/A
|
BIO-37068
|
1
|
|
|
- Description
To monitor DNA migration.
5x DNA coloured loading
buffers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Coloured loading
buffer, Xylene Blue |
2x 1ml
|
N/A
|
BIO-37067
|
1
|
|
|
- Description
To monitor DNA migration.
5x DNA coloured loading
buffers.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Co-Precipitant Buffer |
2ml
|
N/A
|
BIO-37079
|
1
|
|
|
- Composition
(3M Na, 5M Ac) pH 5.2.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Hi-Spec Additive |
3 x 1.2ml
|
|
BIO-37032
|
1
|
|
|
- Product Description
- HiSpec Additive is a
newly-developed compound
from Bioline. This can
eliminate artefacts such as
background smears and
spurious bands, especially
on difficult templates such
as high GC% regions and
repetitive sequences. HiSpec
Additive provides improved
specificity on difficult
templates when used in
conjunction with BIO-X-ACT.
- Features
- • Supplied in 5x
concentration.
• Can be used in almost any
reaction.
• Improves specificity.
- Applications
- • High-specificity
primer extension.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| KCl Buffer |
3 x 1.2ml
|
|
BIO-37027
|
1
|
|
|
- Product Description
- 10x KCl Buffer consists
of: 500mM KCl, 100mM Tris-Cl
(pH 8.8 at 25°C), 15mM MgCl2,
1% Triton X-100.
- Features
- 10x KCl Buffer provides
relaible performance with
the convience of 15mM MgCl2,
which is suitable for most
applications. This makes it
ideal for high-throughput
experiments.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| MgCl2 Solution |
3 x 1.2ml
|
|
BIO-37026
|
1
|
|
|
- Product Description
- MgCl2 50mM in
water, DNase and RNase-free.
- Features
- 50mM MgCl2 is
a convenient concentration
for most molecular
experiments - use 1µl in a
50µl reaction for a final Mg2+
concentration of 1mM.
- Storage Conditions
- MgCl2 can be
stored at 4°C short term and
-20°C for the long term
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| NH4 Buffer |
3 x 1.2ml
|
|
BIO-37025
|
1
|
|
|
- Product Description
- 10x NH4
Buffer consists of: 160mM
(NH4 )2SO4, 670mM
Tris-Cl (pH 8.8 at 25°C),
0.1% Tween-20.
- Features
- 10x NH4
Buffer provides superior
yield with lower background
as compared to KCl-based
buffers. Yields may be up to
2x greater with no loss of
specificity or fidelity.
However, the ammonium
sulfate present in the
buffer may interfere with
downstream applications and
is known to inhibit T4
Polynucleotide Kinase. As a
result, products for
reactions using 10x NH4
Buffer should be purified
after the reaction.
- Storage Conditions
- NH4 Buffer
will remain stable at -20ºC
in a constant-temperature
freezer.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| OptiBuffer |
~200 reactions
|
|
BIO-37030
|
1
|
|
|
| OptiBuffer |
~600 reactions
|
|
BIO-37031
|
1
|
|
|
- Product Description
- OptiBuffer is a novel
non-tris incubation buffer
for advanced polymerase
applications where optimal
conditions are essential to
achieve a high yield and low
background. OptiBuffer
enhances enzyme
thermostability and
increases enzyme half-life
in vitro, leading to a much
improved yield/background
ratio. OptiBuffer provides
an optimal pH stability of
pH 7.8-8.0 at 70°C-72°C,
which is essential for
processivity and for
fidelity of thermostable DNA
polymerases. OptiBuffer
provides a higher buffer
capacity than Tris-HCl-based
buffers. The buffer will
enhance results with all
templates, but is
particularly useful for
difficult templates and for
the synthesis of long
regions. OptiBuffer is
simply substituted for the
incubation buffer supplied
with thermostable
polymerases. It provides the
easiest way to improve the
performance of DNA
polymerase assays.
- Features
- • Improves enzyme
thermostability and
increases enzyme half-life
in vitro.
• Replaces standard buffers,
providing higher DNA yield
without non-specific
background, particularly for
long regions.
- Applications
- • Add to any DNA
polymerase reaction for
improved results.
- Storage Conditions
- OptiBuffer will remain
stable at –20°C in a
constant-temperature
freezer.
- Notes / Protocols
- OptiBuffer is supplied
as a 10x stock solution
which is substituted for the
reaction buffer supplied
with the polymerase in use.
Before use, thaw the frozen
buffer completely and then
dissolve any salt
precipitate by brief
vortexing. Add Mg2+ to give
a final concentration of
1.5mM.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| PCR Optimization Kit |
12 x 1ml Buffers
|
N/A
|
BIO-37051
|
1
|
|
|
- Product Description
- The PCR Optimization Kit
is a complete set of 12
"ready to go" 2X reaction
mixes (A-L), designed to
determine rapidly the
optimal buffer conditions
for all primer-template
combinations. The kit is
designed for use in
"difficult PCR" which often
results from high GC or AT
content, complex secondary
structure, or in multiplex
reactions.
Included in our buffers is a
proprietary mix of enhancers
to ensure the best possible
results are achieved.The PCR
Optimization Kit
dramatically reduces the
time needed to set up
reactions and allows the
user to optimise key
variables without the need
to test each variable
independently.
The user is required to add
only template DNA, primers,
water and thermostable DNA
polymerase to carry out
successful PCR reactions.
Once a specific PCR
Reaction is optimized,
individual buffer(s) can be
purchased separately.
|
|
Step 1
Step 2 |
|
|
Step 3 |
-
Prepare
mastermix
containing
template DNA,
primers, H2O
and thermostable
polymerase.
-
Add 25µl of
mastermix to
each of the 12
buffers supplied
-
Typical results,
buffer J is the
most suitable
for this
application
|
|
- Features
-
- Fast: Rapid
optimisation of PCR
conditions for all
primer-template
combinations.
- Reliable: Provides
greater reproducibility
for primer-template
combinations when the
appropriate 2X Premix is
being used.
- Efficient: Each 2X
Premix contains a
buffered solution,
dNTPs, Magnesium for
rapid optimisation and
the elimination of
background often seen in
PCR.
- Notes
- Each reaction requires
25µl of the supplied 2X
premix, addition of
template, primers,
thermostable DNA Polymerase
and 18.2Ω water up to 50µl.
Taq DNA Polymerase is not
supplied. This product
should be used in
conjunction with a licensed
DNA Polymerase.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Tri-Colour DNA loading
buffer |
2x 1ml
|
N/A
|
BIO-37070
|
1
|
|
|
- Description
Three size approximation.
5x Tri-Colour DNA loading
buffer.
|
|
|
|
|
|
|
| PRODUCT |
SIZE
|
CONC.
|
CAT NO.
|
QUANTITY
|
|
| Water |
10 x 10ml
|
N/A
|
BIO-37080
|
1
|
|
|
| Water |
250ml
|
N/A
|
BIO-37081
|
1
|
|
|
- Composition
Water, 18.2MΩ, PCR-Grade.
Dnase/Rnase free
|
|
|
|
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
