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Complete Culture Media

* Chromosome Medium - Liquid
 
  • The complete medium is standardized for chromosome analysis with excellent lot to lot consistancy.
     
  • INSTRUCTIONS FOR CHROMOSOME ANALYSIS

    Blood samples should be taken (sufficient cannular diameter, reduced aspiration), as to avoid haemolysis.

    1. Under appropriate (sterile) conditions, approx. 0.5 ml (25-30 drops) of heparinized venous whole blood are added and carefully mixed with 8 ml of Chromosome medium B (or 8 ml of Chromosome medium A + 0.08 ml PHA L (cat.no. M 5030) in a tube. Cultivation time should be 72 hrs at +35 - +37°C.

    2. 0.4 ml of Colchicin/Colcemid (10 µg/ml; cat.no. L 6211/L 6221) are added (corresponding to 0.48 µg/ml of medium concentration); incubate for an additional 2 hrs.

    3. Cells are centrifuged for 5 min at approx. 1000 rpm. Remove supernatant and resuspend cells in 5 ml of hypotonic potassium chloride (0.075 M; cat.no. L 6413). Incubate for another 12 min at +35 - +37°C and centrifuge the culture at 1000 rpm for 10 min.

    4. Discard all but 0.25 ml of the supernatant, resuspend cells in the remaining fluid.

    5. Add 5 ml of freshly prepared, ice cold fixative (glacial acetic acid/methanol 1:3) dropwise to the cell suspension while gently moving the flask. Allow resting for 10 min at +2 - +8°C.

    6. Centrifuge at 1000 G for 10 min, remove remaining fixative and resuspend cells in 5 ml ice-cold fixative.

    7. Following another centrifugation, cells will become visible as a slightly cloudy suspension (approx. 0.5 - 1 ml). Drop 3 - 5 drops of cell suspension from a 30 cm distance into a moist, non-greasy slide.

    8. Air-dried slides can be stained readily using the Orcein or Giemsa staining technique.
     


Formulation(external links)
 
 
Pack/Density  
Unit  		  
Cat.no.  
Chromosome Medium A  
without phytohemagglutinin (PHA) L  
Storage: 36 months at -20°C  
 		 - / -   100 ml     F 5013
  
Chromosome Medium B  
with phytohemagglutinin (PHA) L  
Storage: 36 months at -20°C  
 		 - / -   100 ml     F 5023
  

 
* Hybridoma Medium - Liquid
 
  • Optimized for the production of monoclonal antibodies.
     
  • Different from the original formulation, only 0.05 mg/l riboflavin are used due to danger of photo oxidation.

 
 
 
Pack/Density  
Unit  		  
Cat.no.  
Hybridoma medium  Formulation (external links)
without HAT  
with L-glutamine  
with 10% FBS  
 		 - / -   500 ml     F 5515
  

 
* HybridoMed DIF 1000 - Liquid
 
  • Serum free medium for mammalian cells

    The medium was developed to effectively grow hybridomas, but is also suitable for other cell lines. It is based on a 1:1 mixture of Iscove's medium and Ham's F-12, supplemented with transferrin, insulin, and a BSA/oleic acid complex. Myelomas, hybridomas and also other lymphoic and non-lymphoic cell lines are cultivated in Hybridomed and show growth rates comparable to those observed in serum-supplemented media. Successfully cultivated cells include: YAC-1 (mouse T-cell lymphoma), HeLa (human epitheloid cancinoma), BJA-B (human EBV-negative myeloma), BHK 21 (syrian hamster kidney) and L-psv 129 (mouse L-fibroblast). The medium is available for both 5%, and 10% CO2 atmosphere. For use in bioreactors or to cultivate delicate normal cells, we recommend cat.no. F 8035 (CO2 should be 10% to reach physiological pH values). With incubators adjusted at 5% CO2, F 8055 is recommended. This medium has a reduced level of NaHCO3 and contains 3 g/l HEPES additionally.
     
  • Different from the original formulation, only 0.05 mg/l riboflavin is used due to risk of photo oxidative effects
     
  • A trypsin inhibitor (cat.no. L 2183) is required with serum free media after trypsinating!
     


Formulation (external links)
 
 
 
Pack/Density  
Unit  		  
Cat.no.  
HybridoMed DIF 1000 liquid  
with L-glutamine  
with NaHCO3  
for 10% CO2  
Storage: 36 months at -20°C  
 		 - / -   500 ml     F 8035/1
  
HybridoMed DIF 1000 liquid  
with L-glutamine  
with NaHCO3  
and HEPES  
for 5% CO2  
Storage: 36 months at -20°C  
 		 - / -   500 ml     F 8055/1
  

 
* MCDB 153 - Basal Medium
 
  • A trypsin inhibitor (cat.no. L 2183) is required with serum free media after trypsinating!
     
  • The defined medium well supports the growth of normal human keratinocytes if supplemented with EGF, insulin, hydrocortinsone, ethanolamine, and phosphoethanolamine. Instead of these, Fetal Bovine Serum (FBS) can serve as a general supplement. A 5% CO2 atmosphere is recommended.

 
 
 
Pack/Density  
Unit  
Single price  
Cat.no.  
MCDB 153 keratinocyte medium  Formulation (external links)
Basal medium  
Storage: 12 months at +2 - +8°C  
 		 - / -   500 ml     F 8105
  

 
* MCDB 153 - Complete Medium
 

The serum free medium was supplemented ready-to-use, to support the growth of human normal keratinocytes.
 
 
 
Pack/Density  
Unit  
Single price  
Cat.no.  
MCDB 153 Keratinocyte medium  Formulation (external links)
Complete medium  
with 1.76 g/l NaHCO3  
Storage: 36 months at -20°C  
 		 - / -   500 ml     F 8115
  

 
* Octomed - Liquid and Powdered
 
  • Complete Medium for CHO cells
     
  • Serum- and protein free medium designed for the growth of Chinese Hamster Ovary cells. High purity components used to manufacture this medium, provide consistent and reliable results. The formulation makes it an excellent choice for the production and purification of recombinant proteins. Cell densities obtained in octomed are comparable to those of serum-containing media.

 
 
 
Pack/Density  
Unit  
Single price  
Cat.no.  
octomed serum free  
with 2.1 g/l NaHCO3  
without L-glutamine  
Storage: 18 months at +2 - +8°C  
 		 - / -   500 ml     F 8085
  

 
* PFEK-1 - Liquid
 

With this serum and protein free medium, VERO cells proliferate without prior adaptation. VERO cells in protein free culture provide a sensitive substrate for the propagation of human pathogenic viruses. Cells were infected by several viruses (Coxsackie B4, herpes simplex tpye 1 and 2, measles, and polio, type 1 - 3). Virus titres were comparable to those obtained in serum supplemented media. Due to the very high calcium content of the medium, minor precipitates may occasionally be observed; however, this does not influence cell growth rates.
 
 
Pack/Density  
Unit  
Single price  
Cat.no.  
PFEK-1  Formulation (external links)
protein free medium for VERO cells  
Storage: 18 months at +2 - +8°C  
 		 - / -   500 ml     F 8045
  

 
* Sebomed - Liquid
 
  • Medium for Human Sebocytes

    The modfied DMEM/Ham's F-12 (1:1) was optimized to grow the human sebaceous gland cell line SZ95 in vitro . The cell line itself was developed by transfecting primary human facial sebocytes. A defined medium formulation (in particular of the complete version) allows detailed studies under controlled conditions on the influence of hormones, cytokines, lipids, and drugs on human sebocytes. The formulation is patent protected, and available on request.
     
  • For optimal performance, the basal medium need to be supplemented with 0.1 ng/ml Epidermal Growth Factor (EGF rh; cat.no. W 9515040), and 0.1 ml/ml Fetal Bovine Serum (FBS; cat.no. S 0115). Gentamycin sulphate at a concentration of 50 µg/ml should be used to protect against bacterial contamintion.
     
  • sebomed complete medium
    Basal medium supplemented with
    2.5 mM L-alanyl-L-glutamine
    2.0 g/l NaHCO3
    50 mg/l bovine pituitary extract (BPE)
    5 µg/l epidermal growth factor (EGF rh)
    1 g/l fatty acid-free bovine serum albumin (BSA)
    0.15 µM linoleic acid
     

 
 
 
Pack/Density  
Unit  		  
Cat.no.  
sebomed basal medium  Formulation (external links)
Storage: 12 months at +2 - +8°C  
Sebocyte medium, serum-free  
 		 - / -   500 ml     F 8205
  
sebomed complete medium  Formulation (external links)
Storage: 36 months at -20°C  
Basal medium supplemented  
 		 - / -   500 ml     F 8215
  

 
* Start V Medium - Liquid
 

START V is a well defined medium suited for the sensitive application of neuronal cell culture. It is complete without a serum supplement. The medium demonstrates good utility in particular for primary neuronal cells in long-term culture. Cells displayed typical features like morphological differentiation, neurotransmitter synthesis and electro-physiological characteristics.
 
 
Pack/Density  
Unit  		  
Cat.no.  
Start V medium - complete  Formulation (external links)
with L-glutamine  
Storage: 36 months at -20°C  
 		 - / -   500 ml     F 8075
  

 
* TNB 100 Medium - Liquid
 
  • Different from the original formulation, only 0.05 mg/l riboflavin are used due to risk of photo oxidative effects
     
  • The serum-free preparation TNB 100 was optimized to cultivate neuro blastoma x glioma hybrid cells (108 CC 15). To prevent mutations of these cell hybrids, TNB 100 medium should contain HAT supplement (cat.no. F 0483) in the usual concentration. Complete TNB 100 medium is formed by combining 100 ml basal medium and 2 ml of lipid-protein complex. The medium does not contain any trypsin inhibitor. A trypsin inhibitor (cat.no. L 2183; 0.5 mg/ml) is hence required following trypsinsation of cultures. Culture conditions using TNB 100 medium should be: +37°C, 10% CO2, at least 90% relative humidity, and media exchange every 48 hours. TNB 100 (w/o HAT) is well suited for normal neuronal cells (mouse, chick, and rat); however, the preparation may also be useful to study non-neuronal cells such as human cytotoxic T-cells.

 
 
 
Pack/Density  
Unit  		  
Cat.no.  
TNB 100 basal medium  Formulation (external links)
with L-glutamine  
with 3.86 g/l NaHCO3  
Storage -20°C  
Storage: 36 months at -20°C  
 		 - / -   100 ml     F 8023
  
TNB 100 lipid-protein complex lyophilized  
(reconstitute with 2.5 ml sterile water)  
(sufficient for 100 ml)  
Storage: 24 months at +2 - +8°C  
 		 - / -   2,5 ml     F 8820
  

 

 

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Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07