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Cell Culture Diagnostics
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HAT - /HT - Supplement
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Hybridoma selection using HAT/HT
Basal media suitable for lymphoid cells (e.g. RPMI 1640,
Dulbecco's MEM), supplemented with (50x) HAT/HT concentrate, can
serve as selection media for hybridoma cells.
Metabolic precursors, 100 µM
Hypoxanthine (for purine synthesis), 16 µM
Thymidine (for pyrimidine synthesis), both able to serve the
side-pathway, and 0.4 µM
Aminopterine (as a folic acid antagonist, inhibitor of the
main pathway of nucleic acid synthesis) are added to the medium.
By fusing normal lymphocytes with transformed, gene-defect
myeloma cells - e.g. missing
hypoxanthine-guanine-phosphoribosyl-transferase activity (HGPRT-)
and/or missing thymidine kinase activity (TK-
- only hybridomas of both cells can survive, and proliferate.
B-lymphocytes from the spleen of an immunized animal, will
survive only for a few days and do not proliferate, although by
their intact metabolic side pathway (HGPRT+,
HT+
and the addition of hypoxanthine and thymidine, they could
bypass the blockade by aminopterin.
Cells with retained proliferate potential will eventually die
off because of their enzyme deficiency (HGPRT-,
T>K-.
Only hybridomas 'repaired' by genetic complementation (HGPRT+,
TK+
through fusion, will proliferate by the double selection
mechanism like transformed myeloma cells.
Successive change of the medium containing (50x) HAT, and
replacing it by HT concentrate, will revert the aminopterine
blockade.
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HAT-Supplement (50x)
Storage: 36 months at -20°C
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100 ml |
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F 0483
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HT-Supplement (50x)
Storage: 36 months at -20°C
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100 ml |
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F 0493
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Mycoplasma Diagnostic Kit |
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Pack/Density |
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VenorGeM® Mycoplasma Diagnostik Kit
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25 Tests |
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WVGM-025
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only available in selected countries, please
inquire. |
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- / - |
250 Tests |
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WVGM-250
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only available in selected countries, please
inquire. |
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- / - |
100 Tests |
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WVGM-100
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only available in selected countries, please
inquire. |
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- / - |
50 Tests |
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WVGM-050
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only available in selected countries, please
inquire. |
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VenorGeM®-QP
Real-Time PCR-Kit for Rapid Mycoplasma
Detection
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- / - |
100 Tests |
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WVGM-QP-100
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only available in selected countries, please
inquire. |
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- / - |
50 Tests |
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WVGM-QP-050
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only available in selected countries, please
inquire. |
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- / - |
25 Tests |
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WVGM-QP-025
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only available in selected countries, please
inquire. |
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Stains and Indicators |
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Pack/Density |
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Neutral red (1:300 - 0.3%)
Cell counting stain.
Only dead cells will accumulate neutral red
in contrast to viable ones.
Storage: 24 months at +15 - +25°C
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100 ml |
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L 6313
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Phenol red 0.5%
pH indicator for cell culture media
Storage: 24 months at +15 - +25°C
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100 ml |
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L 2203
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Trypan blue 0.5%
in physiol. saline
Cell counting stain.
Only dead cells will accumulate trypan blue
in contrast to viable ones.
Storage: 24 months at +15 - +25°C
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100 ml |
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L 6323
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45