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Antibiotics

			Information
			Cell culture media provide an ideal environment also for rods such as bacteria and fungi. Basically, cell cultures can be protected against contamination by strict adherence to sterile technique; but only the discovery of antibiotics made advanced cell culture possible. However, as antibiotics have a toxic potential, their dosage must closely be monitored. To avoid cross contamination, and to monitor sterility, it is strongly recommended to run cell cultures antibiotics-free from time to time. Antibiotics have a limited shelf life of 3 - 5 days at +37°C. Mode of action: 1. Inhibition of cell wall synthesis, cell wall lysis: Bacitracin, Vancomycin 2. Interference with cell wall permeability: Amphotericin B, Polymyxin, Nystatin 3. Inhibition of cell wall synthesis: Tetracyclines, Aminoglycosides (Gentamycin, Kanamycin, Streptomycin) 4. Blocking of DNA subunit synthesis: Neomycin Antibiotic solutions are 100x concentrated. If not otherwise indicated, the antibiotics are dissolved in physiological saline. Amount of antibiotics to be used: 1 ml/100 ml of medium.

Amphotericin B (Fungizone)

Polyen antibiotic C⁴⁶H⁷³NO²⁰,molecular weight 960.10; efficient against both fungi and yeast contaminations. By complexing with sodium-desoxycholate in phosphate buffer, the low water solubility is improved. Because of precipitations which might occur after freezing and thawing, the liquid preparation is stored at +2 - +8°C and is stable under these conditions for 3 months.

Pack/Density

Unit

Cat.no.


Amphotericin B 250 µg/ml (fungizone) Storage: 3 months at +2 - +8°C	- / -	50 ml	A 2612

Amphotericin B, lyophilized

Pack/Density

Unit

Cat.no.


Amphotericin B, lyophilisiert 250 µg/ml	- / -	1x (6x5 ml)	A 2610

G 418-BC

G 418-BC, cristalline, not sterile (G 418 sulphate), selective antibiotic 10 g, activity > 450 µg/mg; to be dissolved in distilled water and sterile filtered; storage at +15 - +30°C (prepared solution +2 - +8°C), cat.no. A 291-25

G 418-BC, liquid; sterile; 50 mg/ml in distilled water; 50 ml ready-to-use solution; dilute according to application; storage up to 24 months at +2 - +8°C, cat.no. A 2912

Pack/Density

Unit

Cat.no.


G 418-BC cristallin, not sterile Selective antibiotic Storage: +2 - +8°C	- / -	10 g	A 291-25



G 418-BC liquid, sterile Storage: +2 - +8°C Selective antibiotic	- / -	50 ml	A 2912

Gentamycin

Metabolic product of Micromonospora purpurea and Micromonospora echínospora. Mixture of 3 closely related components: C₁ (approx. 40%), C₂ (approx. 40%) and C_1a (approx. 20%). Gentamycin has a broad effectivity.

Pack/Density

Unit

Cat.no.


Gentamycin 10 mg/ml A 2710: Lyophilized - reconstitute with 5 ml of sterilized and bi-distilled water Storage: 24 months at +2 - +8°C	- / -	1x (6x5 ml)	A 2710

	- / -	50 ml	A 2712



Gentamycin Gentamycin Storage: 24 months at +2 - +8°C	- / -	2,5 g	A 271-23

	- / -	10 g	A 271-25

	- / -	25 g	A 271-26

Kanamycin

Aminoglykoside antibiotic made from Streptomyces kanamyceticus. Mixture from 95% Kanamycin A and 5% Kanamycin B + C. Kanamycin-A sulphate: C₁₈H₃₆N₄O₁₁.H₂SO₄, MW 582.58.
1.2 g Kanamycin sulphate = 1 g of Kanamycin base.

Pack/Density

Unit

Cat.no.


Kanamycin 5 mg/ml Storage: 36 months at -20°C	- / -	50 ml	A 2512

Mycoplasma Elimination Reagent

Mycoplasma contamination of cell cultures is a serious and widespread problem. A contaminated culture is usually discarded as treatment with standard antibiotics, is undesirable and an ineffective means of elimination. The mycoplasma reagent Mynox® is a novel anti-mycoplasma agent that integrates selectively into the mycoplasma membrane and compromises its integrity. The result is an osmotic influx that leads to the complete disintegration of the mycoplasma membrane. With mycoplasma eradicated, cells can immediately return to their native morphology and normal proliferation rates.

Mynox®

- the first biological reagent to eliminate mycoplasma by killing them, and not just by inhibiting growth

- effective within one treatment, as mycoplasma are usually destroyed within 1 hour. In cell cultures, Mynox® treatment requires only one passage to eliminate all mycoplasma permanently

- not an antibiotic! The activity is based on a biophysical mechanism, and thus no development of resistant strains. When used as directed, Mynox® is safe in cellcultures and virus stocks

- easily removed after treatment by replacing the culture medium

-stable, sterile, and shipped as a ready-to-use solution in phosphate-buffered saline

Pack/Density

Unit

Single price

Cat.no.


Mycoplasma-Off^TM Desinfiziens für alle Oberflächen 1 Liter-Sprühflasche	- / -	1000 ml	WAOD-1000



Mycoplasma-Off^TM Desinfiziens für alle Oberflächen 5 x 1 Liter Nachfüllpack	- / -	5000 ml	WAOD-5000



Mynox® Mycoplasma Elimination Reagent - Ready-to-use - Effective with only one treatment - No antibiotic; based on a biophysical mechanism - Temperature stable, no additional dosage required - High safety in cell cultures - First biological reagent eliminating mycoplasma	- / -	10 applications	WMYN-1000
	only available in selected countries, please inquire.

	- / -	5 applications	WMYN-500
	only available in selected countries, please inquire.

	- / -	2 applications	WMYN-200
	only available in selected countries, please inquire.

Neomycin / Bacitracin

1 mg / 50 IE/ml
Neomycin:
Aminoglycoside antibiotic from Streptomyces fradiae. Mixture derived from approx. 90% Neomycin B (Framycetin) and approx. 10% Neomycin C. Neomycin has a broad activity spectrum.

Bacitracin:
approx. 60,000 - 65,000 IU/g. Polypeptide antibiotic from Bacillus licheniformis. Mixture from 5 chemically similar components of the bacitracins A, B, D, E and F. Bacitracin A (C₆₆H₁₀₃N₁₇O₁₆S, MW 1422.72) makes up 70% thereof. Bacitracin is prodominantly potent against gram-positive bacteria.

Pack/Density

Unit

Cat.no.


Neomycin / Bacitracin 50 U/ml Storage: 36 months at -20°C	- / -	50 ml	A 2412

Penicillin / Streptomycin, lyophilized

10.000 IE / 10.000 µg/ml
Penicillin:
C₁₆H₁₇N₂NaO₄S, MW 356.40, approx. 1650 IU/mg. 1 Mio IU = 599 mg.
Benzyl-penicillin sodium salt, penicillin G sodium salt, biosynthetic amino acid antibiotic. Penicillin is predominantly potent against gram-positive bacteria.

Streptomycin (-Sulphate)
(C₂₁H₃₉N₇O₁₂)₂.3H₂SO₄, MW 1457.40.
Aminoglycoside antibiotic derived from Streptomyces griseus. Streptomycin sulphate has a wide range of action.

Pack/Density

Unit

Cat.no.


Penicillin G Sodium Salt Storage: 24 months at +2 - +8°C	- / -	25 Mio. IE	A 321-42

	- / -	100 Mio. IE	A 321-44



Penicillin/Streptomycin 10.000 IU / 10.000 µg/ml Lyophilized - reconstitute with 5 ml of sterilized and bi-distilled water Storage: 24 months at +2 - +8°C	- / -	1x (6x5 ml)	A 2210



Streptomycin Storage: 24 months at +2 - +8°C	- / -	25 g	A 331-26

	- / -	100 g	A 331-27

Polymyxin B

Polypeptide antibiotic from Bacillus polymyxa. Polymyxin is only active against gram-negative bacteria.

Pack/Density

Unit

Cat.no.


Polymyxin B approx. 6,000 - 8,000 U/mg Storage: 24 months at +2 - +8°C	- / -	5 Mio. IE	A 231-40

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com

International	+32 (0) 16 58 90 45	+32 (0) 16 50 90 45
France	01 43 25 01 50	01 43 25 01 60
Italy	02 36 00 65 93	02 36 00 65 94
Germany	0241 6085 13140	0241 6085 33033

Antibiotics

Send mail to webmaster with questions or comments about this web site. Copyright © 2005 Gentaur BVBA Last modified: feb-07

Send mail to webmaster with questions or comments about this web site.
Copyright © 2005 Gentaur BVBA
Last modified: feb-07