Product Details: Tumor Necrosis Factor (TNF alpha) human
(RECOMBINANT)
Basic Information
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| Product ID: |
GF-090-3 |
| Product Name: |
Tumor Necrosis Factor (TNF alpha) human
(RECOMBINANT) |
| Unit: |
10µg |
| Category: |
Growth Factors, Cytokines, Receptors
and their Antibodies |
| Subcategory: |
Growth Factors/Cytokines/Enzymes |
Product Details
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| Description: |
Human Tumor necrosis factor (TNF-
alpha), also known as Cachectin, is a cytokine composed
of 157 amino acid residues, having a molecular weight of
17 kDa (monomer). There is 79% homology between human
and murine TNF- alpha, including conserved cysteine
residues which form an intrachain disulfide bridge.
Biological effects of this cytokine include antitumor
activity in vivo and in vitro, activation of
polymorphonuclear leukocytes, antiviral activity, and
the induction of the release if Interleukin-1 or colony
stimulating factors from a variety of sources.
Human TNF- alpha (Cachectin) is produced in
genetically engineered yeast, and purified by sequential
chromatography.
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| Molecular Weight: |
17 KDa |
| Biological Activity: |
The cell cytolytic activity is
determined in the L929 cells (3 x 104 cells/assay) and
it is 2-4 x 108 U/mg.
|
| Formulation: |
At concentration of 1 mg/ml in 0.02M
Tris-HCl/ 0.15 M NaCl (pH 8.0) |
| Purity: |
Over 95% pure by SDS gel
electrophoresis. |
| Storage Conditions: |
Store at -80°C. |
| Stability: |
At least 1 year at -80°C. |
| Temperature: |
-80°C |
| References: |
1. Feinstein, R., Kanety, H., Papa,
M.Z., lunenfeld, B., and Karasik, A., (1993) The Journal
of Biological Chemistry, Vol 268, No. 35, December,
26055-26058
2. Kalthoff, H., Roeder, C., Brockhaus, M., Thiele,
H.G., and Schmiegel, W., (1993) The Journal of
Biological Chemistry, Vol 268, No. 4, February,
2762-2766 3. Chaturvedi, M.M. et al. (1994). J. Biol
Chem. 269, 14575-14583. |
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