Product Details: Basic Fibroblast Growth Factor (FGF 2)
human (RECOMBINANT)
Basic Information
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| Product ID: |
GF-030-3 |
| Product Name: |
Basic Fibroblast Growth Factor (FGF 2)
human (RECOMBINANT) |
| Unit: |
10µg |
| Category: |
Growth Factors, Cytokines, Receptors
and their Antibodies |
| Subcategory: |
Growth Factors/Cytokines/Enzymes |
Product Details
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| Description: |
Human Basic Fibroblast Growth Factor is
a mitogen that stimulates cell growth for such cells as
fibroblasts, endothelial cells, myoblasts, glial cells
and smooth muscle cells. Basic FGF is composed of 146
amino acid residues (pro 1 to ser 146), and is 55%
homologous with acidic FGF, including two conserved
cysteine residues.
Human basic FGF is produced by genetically engineered
yeast, and purified by heparin-Sepharose affinity
chromatography.
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| Molecular Weight: |
17.5 KDa |
| Biological Activity: |
The mitogenic activity of human basic
FGF is identical to natural bovine basic FGF, as
measured by stimulation of 3H-thymidine incorporation
into human foreskin fibroblast cells.
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| Formulation: |
Lyophilized powder |
| Purity: |
Over 95% pure by N-terminal amino acid
sequencing, amino acid composition, HPLC analysis, and
SDS gel electrophoresis. |
| Storage Conditions: |
Store at +4°C. Reconstitution is best
in water or buffer near neutral pH, since it is unstable
in acidic solutions. After reconstitution, storage in
aliquots at -80°C is recommended. |
| Stability: |
Several months, lyophilized or in
solution, at pH 6-7 and +4°C. |
| Temperature: |
+4°C |
| References: |
1. Gospodarowicz, D. (1975) J. Biol.
Chem. 250, 2515-2520.
2. Folkman, J. and Klagsbrun, M. (1987) Science
235,442-447
3. Fox, J., et al. (1996) Journal of Biological
Chemistry 271: 12578-12584. |
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+32 (0) 16
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+32 (0) 16
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