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Product Details: Epidermal Growth Factor (EGF) human (RECOMBINANT)

Basic Information

 


Product ID: GF-010-9
Product Name: Epidermal Growth Factor (EGF) human (RECOMBINANT)
Unit: 1mg
Category: Growth Factors, Cytokines, Receptors and their Antibodies
Subcategory: Growth Factors/Cytokines/Enzymes

 

Product Details

 


Description: Human EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human EGF1-52.

 

Biological Activity: The mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells.

 

Formulation: Lyophilized powder
Purity: Over 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
Storage Conditions: Store at +4°C. Reconstitution is best in water or buffer near neutral pH.
Stability: Several months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C.
Temperature: +4°C
References: 1. Cohen, S. (1962) J, Biol. Chem. 237:1555
2. Gregory, H. (1985) J.Cell Science Supp.. 3:11
3.- George-Nascimento, C. et al. (1988) Biochemistry 27:797-802
 

 

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International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Last modified: feb-07