|
| 0104 |
Turbo Broth™ |
500g |
| 0110 |
Turbo Prime Broth™ |
500g |
| |
Ready-to-Use (liquid) |
|
| 0115 |
Turbo Broth™ |
5 x 500mL |
| |
Ready-to-Use (liquid) |
|
| 0120 |
Turbo Prime Broth™ |
5 x 500mL |
AthenaES's Turbo Broth™:
Ideal for base of
fermentation cultures
Cell yields 4 to
5 times higher than LB Broth
Available in
Animal-Product-Free Media: Turbo
Prime Broth™
Available in
Ready-to-Use Liquid Form
Turbo Broth™ and Turbo Prime Broth™ are proprietary media
formulations used for the cultivation of recombinant strains of
E. coli. Turbo Prime Broth™ is made without
animal-derived raw materials and is APF Certified™. These
media have been developed to improve the yield of recombinant
proteins. The carbon source is glycerol which is not metabolized
into acetic acid. It has a rich nutrient base which provides amino
acids, vitamins, inorganic and trace minerals at levels higher than
those of LB Broth. The medium is buffered at pH 7.2±0.2 with
potassium phosphate to prevent a change in pH and to provide a
source of phosphate. These modifications permit higher cell yields
than can be achieved with LB. For expression of recombinant
proteins, induction should be done at cell densities three times
that of LB Broth. Cell yields are typically 4 to 5 times that of LB
Broth in shake flask cultures depending on the characteristics of
the strain. Plasmid copy numbers are higher, which can also enhance
protein expression. This medium is suitable as the base for
fermentation cultures. Biomass yields will depend on the process
employed and the strain. A typical bench-scale fermentation operated
in a batch mode with an air feed can achieve an OD600 of
10 to 12 depending on the strain.
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