|
| 0105 |
Superior Broth™ |
500g |
| 0111 |
Superior Prime Broth™ |
500g |
| |
Ready-to-Use (liquid) |
|
| 0116 |
Superior Broth™ |
5 x 500mL |
| |
Ready-to-Use (liquid) |
|
| 0121 |
Superior Prime Broth™ |
5 x 500mL |
AthenaES's Superior Broth™:
Excellent for
large-scale plasmid preparations
Cell yields 3 to
4 times that of LB Broth
Available in
Animal-Product-Free Media:
Superior Prime Broth™
Available in
Ready-to-Use Liquid Form
Superior Broth™ and Superior Prime Broth™ are proprietary
medium formulations developed by AthenaES™ for improved expression
of recombinant proteins in E. coli. Superior Prime
Broth™ is made without animal-derived raw materials and is APF
Certified™. The media are complex, rich formulations which
supply a nitrogen source, vitamins, and moderate glucose levels.
The media are buffered at pH 7.2±0.2 to prevent acidification of the
medium during metabolism of the glucose. For expression of
recombinant proteins, induction should be done at cell densities
twice that of LB Broth. Cell yields are typically about 3 to 4
times that of LB Broth in shake flask cultures depending on the
characteristics of the strain. Plasmid copy numbers are the highest
among the AthenaES™ family of media. This medium is an excellent
choice for large-scale plasmid preparations. Biomass yields will
depend on the process employed and the strain.
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