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| 0107 |
Hyper Broth™ |
500g |
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Ready-to-Use (liquid) |
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| 0118 |
Hyper Broth™ |
5 x 500mL |
AthenaES's Hyper Broth™:
Ideal for
fermentation cultures and feed-batch applications
Cell yields 4 to
5 times that of LB Broth
Animal-Product-Free: APF Certified™
Available in
Ready-to-Use Liquid Form
Hyper Broth™ is a proprietary medium formulation developed by
AthenaES™ for improved expression of recombinant proteins in E.
coli. The medium is a complex, rich formulation which supplies
amino acids, vitamins, and glucose at high levels. It is made
without the use of animal-derived raw materials and is APF
Certified™. This medium yields the highest level of biomass of
all AthenaES™ media. The medium is buffered at pH 7.3±0.2 and has
the highest buffering capacity of the Expression Media, which
permits high glucose levels. For expression of recombinant proteins,
induction should be done at cell densities three times that of LB
Broth. Cell yields are typically 4 to 5 times that of LB Broth in
shake flask cultures depending on the characteristics of the
strain. Plasmid copy numbers are higher than for LB, which can
facilitate protein expression. This medium is suitable as the base
for fermentation cultures and is excellent for feed-batch
applications. Biomass yields will depend on the process and the
strain employed. Hyper Broth™ powder is supplied with a separate
Glucose Nutrient Mix.
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
