Glucose M9Y

 

0108	Glucose M9Y™	500g
	Ready-to-Use (liquid)
0119	Glucose M9Y™	5 x 500mL

 

Provides higher yields than traditional formulation of glucose M9

Supplemented with a seperate Glucose Nutrient Mix

Available in Ready-to-Use Liquid Form

Glucose M9Y™ medium, used to cultivate E. coli, is a minimal medium formulation supplemented with yeast extract.  It is based on M9 salts with a pH of 6.9±0.2 and employs glucose as the carbon source.  Alternative carbon sources may be substituted.  Buffering is provided by a sodium-potassium phosphate system.  Ammonium chloride provides a nitrogen source.  Yeast extract is added to improve the protein production capacity of the medium and to permit cultivation of auxotrophic strains by supplying a source of amino acids, vitamins and minerals.  This differs from the traditional formulation of glucose M9, which employs casamino acids.  Our experience has shown that yeast extract typically gives higher yields of recombinant proteins than casamino acids.  Glucose M9Y™ powder is supplied with a separate Glucose Nutrient Mix.

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