| TiterZyme® Kits
(ELISA Kits) |
|
 |
| VEGF
Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-080 |
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
| |
Sandwich EIA Procedure
Assay Range: 15.6-1,000 pg/mL
Time to Answer: 19-25 Hours
|
Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein
initially purified from media conditioned by normal bovine pituitary
folliculo-stellate cells and secreted by a variety of vascularized
tissues. It was subsequently found to be identical to a vascular
permeability factor (VPF), which was previously identified in media
conditioned by tumor cell lines based upon its ability to increase the
permeability of capillary blood vessels. The reported activities of VEGF
include stimulation of endothelial cell growth, angiogenesis and
capillary permeability. VEGF is expressed in many human tumor cells,
including human adenocarcinoma, human pancreatic carcinoma, human
hepatocellular carcinoma, renal cell carcinoma, fibrosarcoma, HL60
promyelocytic leukemia, GS-9L glioma and U937 lymphoma cells. In normal
tissues, VEGF expression has been observed in activated macrophages,
keratinocytes, hepatocytes, smooth muscle cells, Leydig cells, embryonic
fibroblasts and bronchial and choroids plexus epithelium, renal
glomerular visceral epithelium and mesangial cells.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Substrate into the wells and incubate.
6. Pipet Stop Solution and read on a plate reader at 450 nm.
7. Calculate sample concentrations from Standard Curve.
|
| |
human VEGF
(900-080) |
| RANGE |
15.6-1,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
39 in duplicate
|
| SENSITIVITY |
14.04 pg/mL
|
| PRECISION |
2.6-5.8% Intra
2.1-5.3% Inter |
SAMPLE TYPE
recommended dilution |
culture media (1:2-32)
serum (1:2-64) |
| TIME TO ANSWER |
Overnight
|
| SPECIES
SPECIFICITY |
human |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| human VEGF 165 |
100% |
human VEGF 121 |
6.0% |
 0.1% by human: PDGF /
IL-10 / GRO / ET-1 / Big ET-1 / G-CSF / SCF / IL-8 / HGF / ET-3
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45