| Correlate™-EIA (ELISA Kits) |
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| Urinary
Prostacyclin Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
900-025 |
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| 5 x 96 Well Kit |
901-025 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
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Time to Answer: <3 Hours
Simultaneously Measures 6-keto-PGF1alpha and
2,3-dinor-6-keto-PGF1alpha
|
Prostacyclin (PGI2) is involved in platelet aggregation,
vasoconstriction and reproductive functions. However, PGI2
has a half-life of 60 minutes in plasma and only 2 to 3 minutes in
buffer. The production of PGI2 is typically monitored by
measurement of
6-keto-Prostaglandin F1alpha (6k-PGF1alpha)
and 2,3-dinor-6-keto-Prostaglandin F1alpha (2,3d-6k-PGF1alpha).
6k-PGF1alpha is produced by the non-enzymatic hydration
of PGI2 and is further metabolized in urine to the 2,3-dinor
derivative. Methods to measure PGI2 in blood and urine
typically involve HPLC, gas chromatography/mass spectrometry,
radioimmunoassay or enzyme immunoassay. The conversion of PGI2
into
6k-PGF1alpha and 2,3d-6k-PGF1alpha is shown
below.
Assay Designs´ Correlate-EIATM Urinary Prostacyclin (UP)
kit is a competitive immunoassay for the quantitative determination of
the Prostacyclin (PGI2) breakdown products in urine, 6k-PGF1alpha
and 2,3d-6k-PGF1alpha. The kit uses a polyclonal antibody to
bind these two molecules with almost equal affinity in a competitive
manner, both the 6k-PGF1a and 2,3d-6k-PGF1alpha in
the sample or an alkaline phosphatase molecule which has 6k-PGF1alpha
covalently attached to it. |
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1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
|
| |
Urinary
Prostacyclin (900-025 & 901-025) |
| RANGE |
7.81-2,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
39 in duplicate
|
| SENSITIVITY |
6.58 pg/mL
|
| 50% INTERCEPT |
62.9 pg/mL
|
| PRECISION |
2.9-9.1% Intra
3.4-9.6% Inter |
SAMPLE TYPE
recommended dilution |
culture media (1:2)
urine (1:10) |
| TIME TO ANSWER |
<3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| 2,3-dinor-6-keto-PGF1alpha
|
100% |
6-keto-PGF1alpha |
89.1% |
| PGF1alpha |
13.2% |
PGF2alpha |
4.99% |
| 13,14 dihydro-PGF1alpha |
2.55% |
15-keto-PGF2alpha |
0.32% |
| PGE1 |
0.08% |
PGD2 |
0.03% |
| 6,15-diketo-13,14 dihydro-PGF1alpha
|
0.03% |
PGB1 |
0.01% |
| PGE2 |
0.01% |
PGA2 |
0.01% |
| TXB2 |
0.01% |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45