FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Unaffected by the Presence of Other Cytokines
Tumor Necrosis Factor-alpha (TNF-alpha) is a 17.5 kDa, 157 amino acid
protein that is a potent lymphoid factor, which exerts cytotoxic effects
on a wide range of tumor cells and other target cells. TNF alpha has
been suggested to play a pro-inflammatory role and has been detected in
synovial fluid of patients with rheumatoid arthritis. TNF alpha is the
primary mediator of immune regulation. The biosynthesis of TNF alpha is
tightly controlled being produced in extremely small quantities in
quiescent cells, but is a major secreted factor in activated cells.
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Substrate into the wells and incubate.
6. Pipet Stop Solution and read on a plate reader at 450 nm.
7. Calculate sample concentrations from Standard Curve.
human TNF-alpha
(900-099)
mouse TNF-alpha
(900-047)
rat TNF-alpha
(900-086)
RANGE
15.63-1,000 pg/mL
31.25-2,000 pg/mL
390.6-25,000 pg/mL
SAMPLE SIZE
100 µL
50 µL
100 µL
SAMPLES PER 96
WELL KIT
39 in duplicate
39 in duplicate
39 in duplicate
SENSITIVITY
8.43 pg/mL
20.1 pg/mL
420.7 pg/mL
PRECISION
3.6-4.7% Intra
6.0-11.8% Inter
5.3-7.5% Intra
6.2-12.0% Inter
3.9-9.7% Intra
1.0-9.0% Inter
SAMPLE TYPE
recommended dilution
culture media (none)
serum (1:8)
EDTA plasma (1:8)
culture media (1:2)
serum (1:4)
culture media (none)
serum (1:5)
TIME TO ANSWER
4 Hours
4 Hours
2 Hours
SPECIES
SPECIFICITY
human
mouse
rat
human
Compound
Cross Reactivity
Compound
Cross Reactivity
human TNF-alpha
100%
Unaffected by: human
TNF-beta / human sTNF RI / human sTNF RII / mouse TNF-alpha /
mouse sTNF RI / mouse sTNF RII / rat TNF-alpha / porcine
TNF-alpha
mouse
Compound
Cross Reactivity
Compound
Cross Reactivity
mouse TNF-alpha
100%
Unaffected by mouse:
IL-1alpha / IL-1beta / IL-2 / IL-3 / IL-4 / IL-5 / IL-6 / IL-7 /
IL-10 / GM-CSF / IFN-gamma / human TNF-alpha / rat TNF-alpha
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:5)
0.1%
by rat: IL-1beta / IL-6 / Osteopontin / MCP-1 / Leptin /
CINC-1 / CINC-2alpha / CINC-2beta / CINC-3