| Correlate™-EIA (ELISA Kits) |
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|
Testosterone Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-065 |
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| 5 x 96 Well Kit |
901-065 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
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Stable, Color-Coded Liquid Reagents
Time to Answer: 3 Hours
Low Cross Reactivity to Other Steroids
|
Testosterone is the main androgen and one of the most important
androgens secreted into the bloodstream. In males, this testosterone is
produced primarily by the Leydig cells of the testes, whereas in females,
approximately half of all circulating testosterone is derived from
peripheral conversion of androstendione, with a lesser amount being
produced by the ovaries and adrenal glands. Testosterone is responsible
for the development and maintenance of the male secondary sex
characteristics as well as growth-promoting effects. In men, the
determination of testosterone levels is used to evaluate the function of
the testes. Low levels of testosterone are symptomatic in clinical
states such as Klinefelter´s syndrome or hypogonadism. In females, high
levels of testosterone are common in cases of ovarian tumors, breast
cancer or hirsutism.
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1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Conjugate followed by the yellow Antibody into the
wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
|
| |
Testosterone
(900-065) |
| RANGE |
7.81-2,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
39 in duplicate
|
| SENSITIVITY |
5.67 pg/mL
|
| 50% INTERCEPT |
166 pg/mL
|
| PRECISION |
7.8-10.8% Intra
9.3-14.6% Inter |
SAMPLE TYPE
recommended dilution |
culture media (1:2)
saliva (1:20)
Not Recommended for Serum and Plasma Samples
|
| TIME TO ANSWER |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| Testosterone |
100% |
19-hydroxytestosterone |
14.6% |
| Androstendione |
7.20% |
Dehydroepiandrosterone |
0.72% |
| Estradiol |
0.40% |
|
|
| <0.001%:
Dihydrotestosterone / Estriol / Aldosterone / Corticosterone /
Cortisol / Cortisone / Estrone / Progesterone / Pregenolone |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45