Survivin, a recently discovered Inhibitor of Apoptosis (IAP) Protein, is
expressed in the G2/M phase of the cell cycle in a cycle-regulated
manner. At the beginning of mitosis, Survivin associates with the
microtubules of the mitotic spindle in a specific and saturable reaction
that is regulated by microtubule dynamics. Disruption of
survivin-microtubule interactions results in loss of Survivin's
anti-apoptosis function and increased
caspase-3 activity, a mechanism involved in cell death, during
mitosis. It indicates that Survivin may counteract a default induction
of
apoptosis in G2/M phase. The over expression of Survivin in cancer
may overcome this apoptotic checkpoint and favor aberrant progression of
transformed cells through mitosis.
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet the yellow Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet the blue Conjugate into the wells and incubtate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop solution and read plate on a plate reader at 450nm.
9. Calculate sample concentrations from the Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:80)