FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Substance P Sequence:
H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
Substance P is a undecapeptide that displays a number of biological
activities. Substance P is synthesized in the ribosomes as a larger
protein and then enzymatically converted into the active peptide. The
peptide is widely distributed in the peripheral and central nervous
systems of vertebrates, where it is thought to act as a
neurotransmitter. In the peripheral system, Substance P is localized in
the primary sensory neurons and neurons intrinsic to the
gastrointestinal tract.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Conjugate followed by the yellow Antibody into the
wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. EIA: Pipet Stop Solution and read on a plate reader at 405 nm.
CLIA: Read in a suitable luminometer or luminescence detector.
6. Calculate sample concentrations from Standard Curve.
Substance P EIA
(900-018 & 901-018)
Substance P CLIA
(910-018)
RANGE
9.76-10,000 pg/mL
1.95-2,000 pg/mL
SAMPLE SIZE
50 µL
50 µL
SAMPLES PER 96
WELL KIT
38 in duplicate
38 in duplicate
SENSITIVITY
8.04 pg/mL
4.98 pg/mL
50% INTERCEPT
547 pg/mL
72.2 pg/mL
PRECISION
4.5-6.7% Intra
4.2-7.3% Inter
5.6-21.6% Intra
6.3-11.6% Inter
SAMPLE TYPE
recommended dilution
culture media (1:2)
saliva (1:20)
urine (1:8)
serum (1:2)
hep plasma (1:2)
culture media (1:2)
saliva (1:20)
urine (1:8)
serum (1:2)
hep plasma (1:2)
TIME TO ANSWER
3 Hours
Overnight
SPECIES
SPECIFICITY
non-specific
non-specific
Compound
Cross Reactivity
Compound
Cross Reactivity
Substance P
100%
Substance P (3-11)
85.9%
Physalaemin
75.3%
Substance P (4-11)
11.7%
Substance P (7-11)
5.90%
Eledoisin (CLIA only)
1.5%
alpha-Neurokinin
0.80%
beta-Neurokinin
0.20%
Somatostatin
<0.001%
Substance P (1-4)
<0.001%
For more information :: Bioxys and Gentaur
BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
