FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
A rapid and simple method to analyze cells
secreting Smac/DIABLO
Complete kits supplying reagents and negative
control required for 50 tests
Smac/DIABLO has been shown to promote Caspase-9 activation by binding to
inhibitor of apoptosis proteins (IAPs) and removing their inhibitory
activity. Overexpression of this protein increases the sensitivity of
the cell to apoptotic stimuli. Smac/DIABLO is the second mitochondrial
protein, along with cytochrome c, that promotes apoptosis by activating
caspases. Mitochondrial-mediated apoptosis is important to development
and tissue homeostasis. Abnormalities in tissue homeostasis can result
in malignancies. Changes in expression levels of Smac/DIABLO have been
found in a number of human cancers including melanoma and cancers of the
ovary and prostate.
Conjugated anti-Smac/DIABLO is tested for direct Flow Cytometric
analysis of human cells. The unconjugated form of this antibody has been
shown to detect endogenous levels of Smac/DIABLO in human samples.
This monoclonal antibody is produced by immunizing mice with a
recombinant protein to develop a clone cell line. The resulting cell
supernatants are purified using a Protein G column and were conjugated
to FITC fluor under optimum conditions.
Flow cytometric analysis of CytoLight™ anti-Smac/DIABLO-FITC stained
Jurkat cells (untreated or treated with 25 µg/mL Anisomycin for 18 hours
at 37°C) compared to a nonspecific negative control antibody.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
