| Correlate™-EIA (ELISA Kits) |
|
 |
|
Progesterone Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
900-011 |
|
|
|
|
| 5 x 96 Well Kit |
901-011 |
|
|
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
| |
Time to Answer: 3 Hours
Low Cross Reactivity to Other Steroids
All Steps at Room Temperature
Stable, Color-Coded Liquid Reagents
|
Progesterone is a major steroid hormone secreted in large amounts by the
corpus luteum and results from the extracellular conversion of
cholesterol, cholestryl esters, adrenal steroids, prenenolone and
pregnenolone sulfate. Small quantities are also secreted directly from
the adrenal glands. Since sex steroids are not stored, progesterone is
quickly cleared from circulation by extracellular conversion to
androgens or estrogen, or to pregnanediol which is conjugated to
glucuronic acid in the liver and excreted in urine. Reproductive-related
activities such as breast glandular development, the endometrial aspects
of the menstrual cycle and the establishment and maintenance of
pregnancy are mediated by the hormone progesterone. In addition,
progesterone also directs pregnancy-support physiology including changes
in carbohydrate, protein and lipid metabolism, thermoregulation, sodium
reabsorption in renal tubules and the reduction of alveolar and atrial
carbon dioxide partial pressures (PCO2). Progesterone is
involved in cell cycle progression, acts as a neurosteroid to promote
remylination of nerve axons and is used therapeutically to treat
menopause-related symptoms in women.
 |
|
|
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. EIA: Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
|
| |
Progesterone
(900-011 & 901-011) |
| RANGE |
15.62-500 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
38 in duplicate
|
| SENSITIVITY |
8.57 pg/mL
|
| 50% INTERCEPT |
118 pg/mL
|
| PRECISION |
4.9-7.6% Intra
2.7-8.3% Inter |
SAMPLE TYPE
recommended dilution |
culture media (1:10)
saliva (1:10)
serum (1:10)
|
| TIME TO ANSWER |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| Progesterone |
100% |
5alpha-Pregnane-3,20-dione |
100% |
| 17-hydroxyprogesterone |
3.46% |
5-pregnen-3beta-ol-20-one |
1.43% |
| Corticosterone |
0.77% |
4-androstene-3,17-dione |
0.28% |
| Deoxycorticosterone |
0.056% |
DHEA |
0.013% |
<0.001%:
17beta-Estradiol / Estrone / Estriol / Testosterone /
Hydrocortisone / 5alpha-Pregnane-3alpha,20alpha-diol / Danazol
|
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45