FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Time to Answer: 3 Hours
Small Sample Volume: 100 µL
Prostaglandin F2alpha (PGF2alpha) is formed by a
reductase conversion of PGH2, an endoperoxide intermediate of
arachidonic acid. PGF2alpha is often viewed as an antagonist
to
PGE2 due to its opposing effects on the same tissues. As
a potent bronchoconstrictor, PGF2alpha has been implicated in
asthma attacks. Reproductive functions include sperm motility, corpus
luteum regulation and uterine/tubule contractions. These effects have
led to the therapeutic application of PGF2alpha in labor
induction and pregnancy termination. Abnormal levels of PGF2alpha
are associated with pre-eclampsia. The
cyclic nucleotide cGMP is known to serve as a second messenger in a
number of PGF2alpha - mediated activities.
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow
Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read on a plate reader at 405 nm.
6. Calculate sample concentrations from Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
1:20)