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PGE2
| Correlate™-EIA, -CLIA, -FPIA
(ELISA Kits) |
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Prostaglandin E2 Colorimetric
Enzyme Immunoassay (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
900-001 |
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| 5 x 96 Well Kit |
901-001 |
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Prostaglandin E2 Chemiluminescent
Immunoassay (CLIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
910-001 |
|
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Prostaglandin E2 High Sensitivity
Enzyme Immunoassay (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
930-001 |
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| 5 x 96 Well Kit |
931-001 |
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Prostaglandin E2
Fluorescence Polarization Immunoassay (FPIA) Kits |
| Description |
Catalog #
|
Kit Insert
|
MSDS |
A homogeneous, quantitative
method of measuring PGE2 in cell supernatants.
The kit includes bulk packaged reagents for 100 tests of
100 µL volume.
|
920-001 |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
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Only Kits to Measure fg/well
Multiple Kit Formats For Your Convenience
Measure Directly in Media, Serum and Ethanol
Stable Color-Coded Liquid Reagents
Range:
Prostaglandin E2 EIA: 39.1-2,500 pg/mL
Prostaglandin E2 CLIA: 7.8-1,000 pg/mL
Prostaglandin E2 HS-EIA: 7.8-1,000 pg/mL
Sensitivity:
Prostaglandin E2 EIA: 13.4 pg/mL
Prostaglandin E2 CLIA: 6.03 pg/mL
Prostaglandin E2 HS-EIA: 8.25 pg/mL
PGE2 FPIA:
Prostaglandin E2 FPIA: 1,562-100,000 pg/mL
Prostaglandin E2 FPIA: 684 pg/mL
Z-Factor: 0.88
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Prostaglandin E2 (PGE2) is formed from PGH2
which itself is synthesized from arachidonic acid by the enzyme
prostaglandin synthetase. Prostaglandin E2 has been shown to
have a number of biological actions, including vasodilation, both anti-
and proinflammatory action, modulation of sleep/wake cycles and of the
replication of human immuno deficiency virus. Prostaglandin E2
also elevates
cyclic AMP levels, stimulates bone resorption and has
thermoregulatory effects. In addition, Prostaglandin E2 has
been shown to be a regulator of sodium excretion and renal hemodynamics.
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PGE2 EIA
(900-001 & 901-001) |
PGE2
CLIA
(910-001) |
PGE2
HS-EIA
(930-001 & 931-001) |
PGE2
FPIA
(920-001) |
| RANGE |
39.1-2,500 pg/mL
|
7.81-1,000 pg/mL
|
7.8-1,000 pg/mL
|
1,562-100,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
100 µL |
100 µL |
100 µL/96 well
40 µL/384 well |
| SAMPLES PER
KIT |
37 in duplicate/96
well kit |
36 in duplicate/96
well kit |
36 in duplicate/96
well kit |
100 tests at
100 µL |
| SENSITIVITY |
13.4 pg/mL
|
6.03 pg/mL
|
8.26 pg/mL
|
684 pg/mL
|
| 50% INTERCEPT |
222 pg/mL
|
70.8 pg/mL
|
77 pg/mL
|
8,121 pg/mL
|
| Z-FACTOR |
n/a |
n/a |
n/a |
0.88 |
| PRECISION |
5.8-17.5% Intra
3.0-5.1% Inter |
4.1-14.7% Intra
5.6-9.2% Inter |
3.1-9.8% Intra
8.1-12.6% Inter |
8.3-21% Intra
10.7-12.7% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
saliva (1:10)
serum (1:10)
urine (1:10)
whole blood (1:10)
|
culture media (none)
saliva (1:10)
serum (1:10)
urine (1:10)
whole blood (1:10)
|
culture media (none)
saliva (1:10)
serum (1:10)
urine (1:10)
whole blood (1:10)
|
cell supernatants
|
| TIME TO ANSWER |
< 3 Hours
|
3 Hours |
Overnight
|
minimum of 30 minutes
|
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
non-specific
|
non-specific
|
|
| EIA, HS EIA,
CLIA |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| PGE2 |
100% |
PGE1 |
70.0% |
| PGE3 |
16.3% |
PGF1alpha |
1.40% |
| PGF2alpha |
0.70% |
6-keto-PGF1alpha |
0.60% |
| PGA2 |
0.10% |
PGB1 |
0.10% |
| <0.10%:
13,14-dihydro-15-keto-PGF2alpha /
6,15-keto-13,14-dihydro-PGF1alpha / Thromboxane B2
/ 2-Arachidonoylyglycerol / Anandamide / PGD2 /
Arachadonic Acid
|
| FPIA |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| PGE2 |
100% |
PGE1 |
100% |
| PGD2 |
11% |
PGF2alpha |
2.5% |
| 6-keto-PGF1alpha |
2.3% |
PGB2 |
1.8% |
| PGI2
|
0.9% |
TXB2 |
0.3% |
| <0.10%: Arachidonic
Acid / Dihomo-gamma-linolenic acid |
|
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+32 (0) 16
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+32 (0) 16
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