| TiterZyme® Kits
(ELISA Kits) |
|
 |
| p53
Colorimetric (EIA) Kit |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| 96 Well Kit |
900-117 |
|
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Stable, Liquid Reagents
|
The p53 protein is a transcription factor that plays a crucial role as a
tumor suppressor. In normal cells, p53 levels are kept low by
degradation mediated by MDM2. In response to stress such as DNA damage
and hypoxia, wild-type p53 activates genes that lead to cell cycle
arrest and, in the event of severe stress, to apoptosis. The importance
of p53 is illustrated by the presence of p53 mutants in 50% of all human
cancers. When p53 is inactivated by either mutation or deletion, cells
become increasingly vulnerable to malignant transformation. The absence
of a functional p53 protein allows mutations to accumulate that can
cause tumorigenesis. Mutations in p53 lead to a conformational change in
protein structure which results in increased stability of the protein.
Consequently, p53 is frequently found in higher levels in malignant
cells than in normal cells.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop solution and read plate on a plate reader at 450 nm.
6. Calculate sample concentrations from the Standard Curve.
|
| |
p53 (900-117)
|
| RANGE |
0 - 870 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
41 in duplicate
|
| SENSITIVITY |
9 pg/mL |
| PRECISION |
 4.5%
Intra
12.5%
Inter |
SAMPLE TYPE
recommended dilution |
plasma (none)
serum (none)
cell lysates (
1:5)
tissue homogenates (
1:5)
|
| TIME TO ANSWER |
2.5 Hours
|
| SPECIES
SPECIFICITY |
human, mouse, rat
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45