| TiterZyme® Kits
(ELISA Kits) |
|
Other Names: phosphorylated p38, p38 MAPK, MAPK 14, CSBP, SAPK2A,
p38 MAP Kinase, p38-alpha
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|
|
| p38
Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| phospho: 96 Well Kit |
900-101 |
|
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|
| total: 96 Well Kit |
900-102 |
|
|
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
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| |
Stable, Color-Coded Liquid Reagents
Quantitative
Determination of Total and Phosphorylated p38 Mitogen-Activated Protein
Kinase by Colorimetric Immunoassay (713 KB)
Presented by Mark Cameron at Neuroscience in New Orleans, LA - November
2003
|
p38 MAP kinase (MAPK) is also known as mitogen-activated protein kinase
14, MAP kinase p38, p38 alpha, stress activated protein kinase 2A
(SAPK2A), RK, MX12, CSBP1, CSBP2 and cytokine suppressive
anti-inflammatory drug binding protein (CSAID binding protein). There
are many recent reviews in the literature detailing the function and
activity of p38. It is the mammalian counterpart of the yeast HOG kinase
(High Osmolarity Glycerol response kinase). p38 is involved in a
signaling system that controls cellular responses to cytokines and
stress. Similar to the SAPK/JNK
pathway, p38 MAP kinase is activated by a range of cellular stimuli
including osmotic shock, lipopolysaccharides (LPS), inflammatory
cytokines, UV light and growth factors. MKK3 and SEK activate p38 MAP
kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase
has been shown to phosphorylate MAPKAP kinase-2 and the transcription
factors ATF-2 and Max.
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1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Conjugate into the wells and incubate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop Solution and read on a plate reader at 450 nm.
9. Calculate sample concentrations from Standard Curve.
|
| |
phospho p38
(900-101) |
total p38 (900-102)
|
| RANGE |
156-5,000 pg/mL
|
78-5,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
100 µL |
| SAMPLES PER 96
WELL KIT |
40 in duplicate
|
39 in duplicate
|
| SENSITIVITY |
52.1 pg/mL
|
15.6 pg/mL
|
| PRECISION |
6.8-13.8% Intra
6.9-12.1% Inter |
2.6-7.6% Intra
2.3-6.3% Inter |
SAMPLE TYPE
recommended dilution |
RIPA cell lysis buffer
( 1:80)
|
RIPA cell lysis buffer
2 (1:60)
|
| TIME TO ANSWER |
3 Hours |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
|
| phospho p38 |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| phospho p38 |
100% |
|
|
< 0.01%:
non-phosphorylated p38 / phospho JNK / non-phosphorylated JNK /
AKT / non-phosphorylated ERK / phospho ERK
|
| total p38 |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| phospho p38 |
100% |
non-phosphorylated ERK |
3% |
| <0.01%: phospho JNK /
non-phosphorylated JNK / AKT / phospho ERK |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45