| TiterZyme® Kits
(ELISA Kits) |
|
 |
| p21
TiterZyme® (EIA) Kit |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human: 96 Well Kit |
900-126 |
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Stable, Liquid Color-Coded Reagents
Time to Answer: 3 Hours
|
p21, also known as waf-1, cip-1, Sdi1 and CAP-20, is a member of the CKI
(cyclin-dependent kinase inhibitors) family. p21 is an important
indicator of MAPK dependent growth arrest of cells. p21 binds tightly to
the G1 and S phase kinase cyclins A, D and E, blocking their activity
and arresting the cell cycle. While acting as an anti-tumor growth
factor, p21 also acts as an anti-apoptotic agent. Proteins closely
associated with p21 are p27, p17 and
Bcl-2.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet the yellow Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet the blue Conjugate into the wells and incubtate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop solution and read plate on a plate reader at 450nm.
9. Calculate sample concentrations from the Standard Curve.
|
| |
human p21 (900-126)
|
| RANGE |
31.3-1,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
40 in duplicate |
| SENSITIVITY |
5.6 pg/mL |
| PRECISION |
7.3-9.2% Intra
11.1-11.9% Inter |
SAMPLE TYPE
recommended dilution |
RIPA Cell Lysis Buffer
( 1:40)
4 million HeLa cells/mL (1:40)
|
| TIME TO ANSWER |
3 Hours |
| SPECIES
SPECIFICITY |
human |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| p21 |
100% |
|
|
| <0.01%: cIAP1
/ p38 / pMEK / JNK / Beta-Catenin / p300 / Smac/DIABLO |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45