| Correlate™-EIA (ELISA Kits) |
IMPORTANT: We have
recently released a new Oxytocin Correlate™ EIA kit. Due to the
supply of a critical raw material, this assay kit has been
re-developed using a new detection antibody. Effective
immediately, the new format Oxytocin EIA kit (900-153) will be
replacing the older version (900-024).
The specifications for the kit have changed slightly and are
outlined in the attached document:
Oxytocin Technical Bulletin
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| Oxytocin
Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
900-153 (formerly
900-024) |
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| 5 x 96 Well Kit |
901-153 (formerly
901-024) |
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FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
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Stable Color-Coded Liquid Reagents
Oxytocin Range: 15.6-1,000 pg/mL
Oxytocin Sensitivity: 11.7 pg/mL
|
Oxytocin is a peptide, which is produced in the paraventricular nuclei
of the hypothalamus and is stored in the posterior pituitary. Oxytocin
consists of nine amino acids linked with a [1-6] disulfide bond and a
carboxyamidated tail. A hormone once thought to be limited to female
smooth muscle reproductive physiology, more current findings have
determined that oxytocin also functions as a neurotransmitter, may be
involved in neuropsychiatric disorders, social/sexual behavior and is
important in male reproductive physiology. Oxytocin and the related
peptide,
Arg8-Vasopressin, maintain renal water and sodium balance.
Highly conserved across species boundaries, oxytocin-like
neurohypophysial peptides are substituted primarily at residues 4 and/or
8. Acting in classical endocrine fashion, Oxytocin elicits regulatory
effects by binding specific cell surface receptors which in turn
initiate a secondary intracellular response cascade via a
phosphoinositide signaling pathway.
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Oxytocin (900-153 &
901-153) |
| RANGE |
15.6-1,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
37 in duplicate
|
| SENSITIVITY |
11.7 pg/mL
|
| 50% INTERCEPT |
123 pg/mL
|
| PRECISION |
8.7-12.4% Intra
5.2-14.5% Inter |
SAMPLE TYPE
recommended dilution |
culture media (neat)
human breast milk (1:2)
|
| TIME TO ANSWER |
Overnight
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| SPECIES
SPECIFICITY |
non-specific
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|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| Mesotocin |
131% |
Oxytocin |
100% |
| Arg8-Vasopressin |
42.2% |
Oxytocin-SH |
15.4% |
| Arg8-Vasopressin-SH |
0.6% |
Lys8-Vasopressin-SH |
0.6% |
| <0.04%: TRH /
Somatostati / Met-Enkephalin / VIP / Lys8-Vasopressin
/ Arg8-Vasopressin / Ser4, Ile8-Oxytocin
/ alpha-ANP / Growth Hormone |
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+32 (0) 16
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+32 (0) 16
50 90 45