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Nitric Oxide and Total NO

Nitric Oxide (regular & total)
Detection Kits
NEW!: Our Total Nitric Oxide Assay Kit now provides sufficient reagents to perform 2x96 determinations. Two ready to use microtiter plates are now included at the same price. This change will not affect the performance of this kit.
 
Detection Kits
Format Catalog #
Kit Insert
MSDS
Assay Layout
Citations
Regular: 2 x 96 Determination Kit 917-010 (formerly 907-010)
assay insert
Total: 2 x 96 Determination Kit 917-020 (formerly 907-020)

FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.


Two Kit Formats to Meet Your Scientific Needs
Simple Microtiter Plate Method
Most Stable Kits Available
Stable, Easy-to-Use Reagents
Convenient Colorimetric Detection at 550 nm
 
 


Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic, and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase. As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates. As a messenger molecule, NO covalently interacts with target molecules based on redox potential rather than noncovalent complementarity.
 


 


Nitric oxide (NO) undergoes a number of reactions under biological conditions. These reactions tend to involve the formation of both nitrite and nitrate ions. Because of the different amounts of nitrite and nitrate formed, in some circumstances, it might be beneficial to determine both nitrite and nitrate concentrations in samples.




 



 




 
 
Nitric Oxide NO2/NO3
(917-010)
Total Nitric Oxide
(917-020)
FORMAT
Nitrite
Nitrate
Total NO
RANGE
3.125-200 µmole/L
3.125-100 µmole/L
3.125-100 µmole/L
SAMPLE SIZE
50 µL
50 µL
50 µL
SAMPLES PER 96 WELL PLATE
39 in duplicate
40 in duplicate
40 in duplicate
SENSITIVITY
0.222 µmole/L
0.625 µmole/L
0.625 µmole/L
PRECISION
0.8-2.4% Intra
1.3-7.7% Inter
0.8-1.5% Intra
2.8-5.5% Inter
1.2-5.3% Intra
3.3-6.9% Inter
SAMPLE TYPE
recommended dilution
culture media (1:2)
saliva (1:2)
urine (1:2)
serum (1:2)
citrate plasma (1:2)
EDTA plasma (1:2)
heparin plasma (1:2)
reaction buffer (1:2)
 
culture media (1:2)
saliva (1:2-100)
urine (1:20)
serum (1:2-20)
citrate plasma (1:2-20)
EDTA plasma (1:2-20)
heparin plasma (1:2-20)
reaction buffer (1:2)
 
culture media
(1:2)
saliva
(1:2-100)
urine
(1:20)
serum
(1:2-20)
citrate plasma
(1:2-20)
EDTA plasma
(1:2-20)
 
SPECIES SPECIFICITY
non-specific
non-specific
non-specific

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Copyright © 2005 Gentaur BVBA
Last modified: feb-07