NEW!: Our Total Nitric
Oxide Assay Kit now provides sufficient reagents to perform 2x96
determinations. Two ready to use microtiter plates are now
included at the same price. This change will not affect the
performance of this kit.
Detection Kits
Format
Catalog #
Kit Insert
MSDS
Assay Layout
Citations
Regular: 2 x 96 Determination Kit
917-010 (formerly
907-010)
Total: 2 x 96 Determination Kit
917-020 (formerly
907-020)
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Two Kit Formats to Meet Your Scientific Needs
Simple Microtiter Plate Method
Most Stable Kits Available
Stable, Easy-to-Use Reagents
Convenient Colorimetric Detection at 550 nm
Nitric oxide (NO) is a major mammalian secretory product that initiates
host defense, homeostatic, and developmental functions by either direct
effect or intercellular signaling. NO is the product of a five-electron
oxidation of the amino acid L-arginine mediated by nitric oxide
synthase. As a direct effector, NO is thought to activate regulatory
proteins, kinases and proteases that are directed by reactive oxygen
intermediates. As a messenger molecule, NO covalently interacts with
target molecules based on redox potential rather than noncovalent
complementarity.
Nitric oxide (NO) undergoes a number of reactions under biological
conditions. These reactions tend to involve the formation of both
nitrite and nitrate ions. Because of the different amounts of nitrite
and nitrate formed, in some circumstances, it might be beneficial to
determine both nitrite and nitrate concentrations in samples.