| TiterZyme® Kits
(ELISA Kits) |
|
 |
| human
Myeloperoxidase (EIA) Kit |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human: 96 Well Kit |
900-115 |
|
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|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Stable, Color-Coded Reagents
An
Enhanced Sensitivity Colorimetric Immunoassay for the Measurement of
Myeloperoxidase (712 KB)
Presented by Faye Bradbury at Neuroscience in New Orleans, LA - November
2003
|
Myeloperoxidase (MPO) is synthesized as a single chain precursor, which
is then processed into a tetramer consisting of 2 light chains and 2
heavy chains. In response to microbial invasion, myeloperoxidase is
released from the cytoplasmic granules of neutrophils into the
extracellular space and phagosome, where it generates hypohalous acids
that have a strong microbicidal effect. Since myeloperoxidase is a major
component of myeloid precursor cells, the enzymatic activity of
myeloperoxidase is used to classify acute leukemias. Myeloperoxidase is
used also as a marker of airway inflammation caused either by disease,
such as asthma, or environmental irritants. In addition to the protein
itself, autoantibodies against myeloperoxidase are markers for several
systemic vasculitis diseases.
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|
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1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the yellow Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet the blue Conjugate into the wells and incubate.
5. Aspirate and wash the plate.
6. Pipet Substrate into the wells and incubate.
7. Pipet Stop Solution and read on a plate reader at 450 nm.
8. Calculate sample concentrations from Standard Curve.
|
| |
Myeloperoxidase
(900-115) |
| RANGE |
0.39-12.5 ng/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
40 in duplicate
|
| SENSITIVITY |
0.13 ng/mL
|
| PRECISION |
2.8-5.4% Intra
5.9-8.8% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
sputum supernatant ( 1:16)
nasal lavage supernatant (1:2)
heparin plasma (1:16)
urine (1:8)
|
| TIME TO ANSWER |
< 3 Hours
|
| SPECIES
SPECIFICITY |
human |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| human Myeloperoxidase |
100% |
human Erythropoietin |
1.1% |
| bovine Lactoperoxidase |
0.40% |
human Elastase |
0.20% |
| human Lactoferrin |
0.20% |
sheep COX-2 |
0.10% |
| human Thyroid Peroxidase |
<0.10% |
human Eosinophil-derived Neurotoxin |
<0.10% |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45