|
| |
Mouse IgG
| TiterZyme® Kits
(ELISA Kits) |
|
 |
| mouse IgG
Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| mouse IgG1: 96 Well Kit |
900-109 |
|
|
|
| mouse IgG2a: 96 Well Kit |
900-113 |
|
|
|
| mouse IgG2b: 96 Well Kit |
900-110 |
|
|
|
| mouse IgG3: 96 Well Kit |
900-121 |
|
|
|
| mouse IgM: 96 Well Kit |
900-120 |
|
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Ready-to-Use Plate and Reagents
Time to Answer: 90 minutes
|
IgG is divided into four subclasses: IgG1, IgG2,
IgG3 and IgG4. IgG is the most abundant
immunoglobulin found in the blood. IgG is a glycoprotein which consists
of two identical heavy chains (50 kDa each) and two identical light
chains (25 kDal each) to give a combined mass of about 150 kDa. The
chains are held in place by covalent disulfide bonds. Each light chain
contains two immunoglobulin (Ig) domains, while the heavy chains contain
four Ig domains each. In the middle of each heavy chain is a relative
varying portion called the "hinge region", which is unique to each IgG.
This region allows for molecular flexibility. IgG1 properties
and functions include neutralization, opsonization, activation of the
complement system, diffusion into extravascular sites and crossing the
placenta. IgG2 properties also include neutralization and
diffusion into extravascular sites.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Pipet the Conjugate into the wells and incubtate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. Pipet Stop Solution and read plate on a plate reader at 450nm.
6. Calculate sample concentrations from the Standard Curve.
|
| |
mouse IgG1
|
mouse IgG2a
|
mouse IgG2b
|
mouse IgG3
|
mouse IgM
|
| RANGE |
7.81-250 ng/mL
|
7.81-250 ng/mL
|
7.81-250 ng/mL
|
7.81-250 ng/mL
|
3.91-250 ng/mL
|
| SAMPLE SIZE |
50 µL |
50 µL |
50 µL |
50 µL |
50 µL |
| SAMPLES PER 96
WELL KIT |
40 in duplicate
|
40 in duplicate
|
40 in duplicate
|
40 in duplicate
|
40 in duplicate
|
| SENSITIVITY |
1.79 ng/mL
|
318.8 pg/mL
|
1.05 ng/mL
|
0.901 ng/mL
|
0.60 ng/mL
|
| PRECISION |
3.4-4.4% Intra
3.5-6.5% Inter |
1.2-2.1% Intra
4.1-4.8% Inter |
4.5-6.2% Intra
5.4-7.9% Inter |
4.5-6.5% Intra
12-19% Inter |
2.0-6.7% Intra
5.0-8.5% Inter |
SAMPLE TYPE
recommended dilution |
serum ( 1:20,000)
culture media (none) |
serum (1:100,000)
culture media (none) |
serum (1:100,000)
culture media (none) |
culture media (none)
serum (1:64,000)
|
serum (1:25,000)
culture media (none)
ascites fluid (1:2,500)
|
| TIME TO ANSWER |
90 Minutes
|
90 Minutes
|
90 Minutes
|
90 Minutes
|
90 Minutes
|
| SPECIES
SPECIFICITY |
mouse |
mouse |
mouse |
mouse |
mouse |
|
|
mouse IgG1 |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| mouse IgG1 |
100% |
rat IgG1 |
0.90% |
| mouse IgG2b |
0.21% |
|
|
| <0.01%: human IgG1,
mouse IgG2a, mouse IgG3, mouse IgM |
|
mouse IgG2a |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| mouse IgG2a |
100% |
mouse IgG3 |
0.49% |
| mouse IgM |
0.12% |
|
|
| <0.01%: rat IgG2a,
mouse IgG1, mouse IgG2b |
|
mouse IgG2b |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| mouse IgG2b |
100% |
|
|
| <0.10%: rat IgG2b,
mouse IgG1, mouse IgG2a, mouse IgG3,
mouse IgM
|
|
mouse IgG3 |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| mouse IgG3 |
100% |
|
|
| <0.01%: mouse IgG1,
mouse IgG2a, mouse IgG2b, mouse IgM |
| mouse IgM |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| mouse IgM |
100% |
rat IgM |
60% |
| human IgM |
<0.4% |
mouse IgG1 |
<0.2% |
| <0.10%: mouse IgG2a
/ mouse IgG2b / mouse IgG3 |
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45