| TiterZyme® Kits
(ELISA Kits) |
phosphorylated and total MEK 1 |
 |
| phospho &
total MEK 1 Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| phospho: 96 Well Kit |
900-119 |
|
|
|
| total: 96 Well Kit |
900-122 |
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
| |
Stable, Color-Coded Liquid Reagents
|
MEK1 is also known by a variety of other names. They include dual
specificity mitogen-activated protein kinase kinase (MAP kinase kinase 1
or MAPKK 1), ERK activator kinase 1, MAPK/ERK kinase 1, ERK kinase 1 and
MAP kinase kinase. MEK is a 393 amino acid, 43.5 kDa protein that is
highly conserved in evolution. MEK phosphorylates threonine and tyrosine
residues on MAP kinases
ERK 1 and 2 (p44 and p42 MAP kinase). MEK participates in a wide
range of cellular processes including cell proliferation,
differentiation and apoptosis. MEK1 is activated by phosphorylation of
Ser218 and Ser222 by the serine-threonine kinase RAF1, which is part of
the p21ras signal transduction pathway. Constitutive activation of MEK1
results in cellular transformation. This protein kinase has been
reported to be a likely target for pharmacological intervention in
proliferative diseases. Recent literature reviews cover MEK activity in
great detail.
|
 |
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Conjugate into the wells and incubate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop Solution and read on a plate reader at 450 nm.
9. Calculate sample concentrations from Standard Curve.
|
| |
phospho MEK 1
(900-119) |
total MEK 1
(900-122) |
| RANGE |
187.5 - 6,000 pg/mL
|
312.5 - 10,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
100 µL |
| SAMPLES PER 96
WELL KIT |
40 in duplicate
|
40 in duplicate
|
| SENSITIVITY |
85.2 pg/mL
|
181.4 pg/mL
|
| PRECISION |
3.4 - 4.7% Intra
3.9 - 14.8% Inter |
1.7 - 4.9% Intra
5.4 - 7.5% Inter |
SAMPLE TYPE
recommended dilution |
1 million Jurkat cells
per mL (none)
2 million Jurkat cells per mL (1:2)
4 million Jurkat cells per mL (1:4)
|
1 million Jurkat cells
per mL (1:4-32)
2 million Jurkat cells per mL (1:8-32)
4 million Jurkat cells per mL (1:16-32)
|
| TIME TO ANSWER |
3 Hours |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| phospho MEK
1 |
| pMEK 1 |
100% |
pMEK 2 |
12.2% |
| <0.1%: MEK-1 (inactive)
/ phospho JNK / ERK2 / phospho ERK2 |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| total MEK 1
|
| pMEK 1 |
100% |
pMEK 2 |
25.5% |
| <0.1%: pJNK / ERK2 /
GSK-3beta / p38 |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45