FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.
Unaffected by the Presence of Other Chemokines
Assay Range: 50-1,600 pg/mL
Time to Answer: 2 Hours
Sandwich EIA Procedure
Monocyote Chemoattractant Protein-1 (MCP-1) is a basic protein
consisting of 76 amino acids and is classified as C-C sub family of
chemokines. MCP-1 shows specific chemotaxis to the monocyote and relates
to the manifestation of desmosomal molecule on the surface of monocytes,
the monocyte chemotaxis to the inflammation site, conjugation with
endothelial cell and to the exudation to sub-endothelium. It is produced
in chronic inflammatory diseases such as chronic arthritis and nephritis
and in malignant tumors such as atherosclerosis and others and is
thought to have an important role to the regional exudation of monocyte
and macrophage.
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Substrate into the wells and incubate.
6. Pipet Stop Solution and read on a plate reader at 450 nm.
7. Calculate sample concentrations from Standard Curve.
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
