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LTB4

LTB4
Correlate™-EIA, -CLIA™ (ELISA Kits)
Leukotriene B4 spacer
Leukotriene B4 Colorimetric (EIA) Kits
Assay Format Catalog #
Kit Insert
MSDS
Assay Layout
Citations
96 Well Kit 900-068
LTB4 assay insert
LTB4 MSDS
LTB4 assay layout
5 x 96 Well Kit 901-068
Leukotriene B4 assay insert
Leukotriene B4 MSDS
Leukotriene B4 assay layout
Leukotriene B4 Chemiluminescent (CLIA) Kits
Assay Format Catalog #
Kit Insert
MSDS
Assay Layout
Citations
96 Well Kit 910-068
Leukotriene B4 CLIA assay insert
Leukotriene B4 CLIA MSDS
Leukotriene B4 CLIA assay layout
Leukotriene B4 spacer
Leukotriene B4 line
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE.

Leukotriene B4 featuresLeukotriene B4 back to top
 
Assay Designs assay components
Low Cross Reactivity to other Eicosanoids
 

Leukotriene B4 usesLeukotriene B4 back to top

Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism formed via the 5-lipoxygenase pathway. Leukotriene B4 stimulates leukocyte functions including lysosomal enzyme release, adhesion and aggregation of polymorphonuclear leukocytes. A potent mediator of inflammatory diseases and immunoregulation, Leukotriene B4 also increases the cytotoxic activity of human natural killer cells. Elevated levels of Leukotriene B4 are associated with bronchial vasoconstriction and increased intracellular calcium levels.

 


Leukotriene B4 structure

Leukotriene B4 curvesLeukotriene B4 back to top
 

Leukotriene B4 EIA graph
Leukotriene B4 CLIA graph
 

Leukotriene B4 proceduresLeukotriene B4 back to top
1. Pipet Standards and Samples in duplicate into the wells.
2. Pipet the blue Alkaline Phosphatase Conjugate followed by the yellow Antibody into the wells and incubate.
3. Aspirate and wash the plate.
4. Pipet Substrate into the wells and incubate.
5. EIA: Pipet Stop Solution and read on a plate reader at 405 nm.
CLIA: Read in a suitable luminometer or luminescence detector.
6. Calculate sample concentrations from Standard Curve.
 

 

Leukotriene B4 performanceLeukotriene B4 back to top

 
 
LTB4 EIA (900-068 & 901-068)
LTB4 CLIA (910-068)
RANGE
11.7-3,000 pg/mL
15.6-4,000 pg/mL
SAMPLE SIZE
100 µL
100 µL
SAMPLES PER 96 WELL KIT
39 in duplicate
39 in duplicate
SENSITIVITY
5.63 pg/mL
12.95 pg/mL
50% INTERCEPT
98 pg/mL
112.4 pg/mL
PRECISION
5.9-6.8% Intra
5.0-16.5% Inter
6.8-10.9% Intra
6.1-11.7% Inter
SAMPLE TYPE
recommended dilution
culture media (none)
saliva (1:4)
urine (none)
EDTA plasma (1:2-1:4)
 
culture media (none)
saliva (1:16)
urine (1:16)
EDTA plasma (1:8)
hep plasma (1:8)
 
TIME TO ANSWER
4 Hours
4 Hours
SPECIES SPECIFICITY
non-specific
non-specific

Leukotriene B4 reactivitiesLeukotriene B4 back to top 

 
Compound Cross Reactivity Compound Cross Reactivity
LTB4  100% 6-trans-12-epi-LTB4 5.50% 
6-trans-LTB4  4.90% 12-epi-LTB4  0.94%
<0.20%:20-OH-LTB4 / PGE2 / PGF2alpha / 20-COOH-LTB4 / LTC4 / LTD4 / LTE4 / 5(S)-HETE / 12(S)-HETE / 15(S)-HETE

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07