| TiterZyme® Kits
(ELISA Kits) |
phosphorylated and total JNK1 |
 |
| JNK1/2
Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| phospho: 96 Well Kit |
900-106 |
|
|
|
| total: 96 Well Kit |
900-107 |
|
|
|
|
|
| |
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
| |
Stable, Color-Coded Liquid Reagents
|
The c-Jun N-terminal protein kinase (JNK1) mitogen-activated protein
kinases (MAP
Kinases) are an evolutionarily-conserved family of serine/threonine
protein kinases. JNK1 is also known as stress-activated protein kinase (SAPK).
Protein kinases act in a signaling system by which cells transduce
extracellular stimuli into intracellular responses. Activation of JNK1
occurs by phosphorylation at Thr183 and Tyr185 by SEK/MKK4. These
stimuli include UV radiation, inflammatory cytokines, osmotic stress and
sheer stress. JNK1, when active, can translocate to the nucleus where it
regulates transcription through its effects on c-Jun, ATF-2 and other
transcription factors.
|
 |
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Conjugate into the wells and incubate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop Solution and read on a plate reader at 450 nm.
9. Calculate sample concentrations from Standard Curve.
|
| |
phospho JNK1/2
(900-106) |
total JNK1/2
(900-107) |
| RANGE |
125 - 4,000 pg/mL
|
31.25 - 2,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
100 µL |
| SAMPLES PER 96
WELL KIT |
40 in duplicate
|
39 in duplicate
|
| SENSITIVITY |
75.8 pg/mL
|
11.4 pg/mL
|
| PRECISION |
4.0 - 8.2% Intra
5.6 - 6.5% Inter |
3.7 - 7.8% Intra
4.3 - 9.2% Inter |
SAMPLE TYPE
recommended dilution |
RIPA cell lysis buffer
( 1:8)
|
1% SDS/Cell Lysate (1
million cells/mL) (1:160)
1% Triton X-100/Cell Lysate (1 million cells/mL) (1:160)
|
| TIME TO ANSWER |
3 Hours |
3 Hours |
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
|
WARNING: If the end user chooses to not use the
provided RIPA Cell Lysis Buffer, it is up to the end user to determine
the appropriate dilution of samples and assay validation for their
chosen cell lysis buffer.
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| phospho JNK
|
| phospho JNK1 |
100% |
phospho JNK2 |
98.6% |
| phospho p38 |
0.42% |
phospho ERK2 |
0.16% |
| non-phosphorylated ERK2 |
<0.01% |
non-phosphorylated JNK1 |
<0.01% |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| total JNK
|
| JNK1 |
100% |
phospho JNK1 |
129% |
| phospho JNK2 |
1.94% |
--- |
--- |
| <0.01%: phospho ERK2 /
ERK2 / phospho AKT / AKT / p38 |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45