| TiterZyme® Kits
(ELISA Kits) |
|
 |
|
Interleukin-8 Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human: 96 Well Kit |
900-035 |
|
|
|
|
|
| |
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Unaffected by the Presence of Other Cytokines
Time to Answer: <3 Hours
Small Sample Volume: 100 µL
|
Interleukin-8 (IL-8) is a CXC chemokine in which an amino acid is
present between the first two of four highly conserved cysteine residues.
Monocytes, macrophages, neutrophils, lymphocytes, dermal fibroblasts,
keratinocytes, vascular endothelial cells, melanocytes, hepatocytes and
various tumor cell lines, produce IL-8. Human IL-8 functionality is
homologous with mouse, guinea pig, rat and rabbit neutrophils. IL-8 is
present in the synovial fluids of patients with rheumatoid arthritis and
has been purified from plaque psoriasis scales. It is associated also
with sepsis, asthma and glomerulonephritis. The cytokine will be
clinically important in understanding disease states that are
characterized by neutrophil infiltration. |
|
|
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet Conjugate into the wells and incubate.
6. Aspirate and wash the plate.
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop Solution and read on a plate reader at 450 nm.
9. Calculate sample concentrations from Standard Curve.
|
| |
human IL-8
(900-035) |
| RANGE |
15.62-1,000 pg/mL
|
| SAMPLE SIZE |
100 µL |
| SAMPLES PER 96
WELL KIT |
37 in duplicate
|
| SENSITIVITY |
8.22 pg/mL
|
| PRECISION |
3.2-9.4% Intra
3.7-6.9% Inter |
SAMPLE TYPE
recommended dilution |
culture media (none)
serum (none)
EDTA plasma (none) |
| SPECIES
SPECIFICITY |
< 3 Hours
|
| SPECIES
SPECIFICITY |
human |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| human IL-8 |
100% |
|
|
| Unaffected by human:
IL-1alpha / IL-1beta / IL-2 / IL-3 / IL-4 / IL-6 / IL-7 / IL-10
/ IL-11 / TNF-alpha / TNF-beta / GM-CSF / SCF |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45