 |
 |
|
Interleukin-1beta Colorimetric (EIA) Kits |
| Assay Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
| human: 96 Well Kit |
900-130 (formerly 900-029) |
|
|
|
| mouse: 96 Well Kit |
900-132 (formerly 900-041) |
|
|
|
| rat: 96 Well Kit |
900-131 (formerly 900-076) |
|
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
|
Unaffected by the presence of other cytokines
Small Sample Volumes
|
Interleukin-1 (IL-1) is a family of related proteins that are considered
to act as the prototypic multifunctional cytokine. Consisting of
IL-1alpha, IL-1beta and IL-1R-a (receptor antagonist), IL-1 elicits
biological responses when present in lower pico- to femto-molar
concentrations. IL-1beta is translated as a 31 kDa precursor that is
glycosylated and cleaved into a cytosolic pro-IL-1beta molecule. The
biologically active 17 kDa fragment is released by enzymatic activity of
the IL-1beta-converting enzyme and secreted from the cell via ABC
transporters. IL-1beta with the aid of inducible transcription factors
initiates the expression of a pro-inflammatory cascade of proteins and
cytokines by selective regulation of other molecules. The broad range of
regulatory events mediated by IL-1beta include but are not limited to
the induction of chemokines, suppression of constitutive housekeeping
genes, initiation of
Prostaglandin E2 synthesis, the expression of inducible
nitric oxide synthase,
soluble PLA2 and
COX-2 as well as bone and cartilage remodeling. IL-1beta is
expressed by a variety of cells including macrophages, monocytes,
platelets and neutrophils. In addition, IL-1beta production has been
reported in NK cells, endothelial cells, fibroblasts, T cells,
osteoblasts and a number of cells that comprise the central nervous
system.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells and incubate.
2. Aspirate and wash the plate.
3. Pipet Antibody into the wells and incubate.
4. Aspirate and wash the plate.
5. Pipet blue Conjugate into the wells and incubate.
6. Aspirate and wash the plate. (h,m)
7. Pipet Substrate into the wells and incubate.
8. Pipet Stop Solution and read on a plate reader at 450 nm.
9. Calculate sample concentrations from Standard Curve.
|
| |
human IL-1beta
(900-130) |
mouse IL-1beta
(900-132) |
rat IL-1beta
(900-131) |
| RANGE |
10.24-400 pg/mL
|
15.6-1,000 pg/mL
|
25.6-2,500 pg/mL
|
| SAMPLE SIZE |
100 µL |
50 µL |
100 µL |
| SAMPLES PER 96
WELL KIT |
42 in duplicate
|
42 in duplicate
|
40 in duplicate
|
| SENSITIVITY |
<1 pg/mL |
<3 pg/mL |
<12 pg/mL
|
| PRECISION |
<10% Intra
<10% Inter |
<10% Intra
<10% Inter |
|
SAMPLE TYPE
recommended dilution |
Serum
Plasma
Urine |
|
Serum
EDTA Plasma
Citrate Plasma
Heparin Plasma |
| TIME TO ANSWER |
< 4 Hours
|
< 4 Hours
|
< 4 Hours
|
| SPECIES
SPECIFICITY |
human |
mouse |
rat |
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| human |
| human IL-1beta |
100% |
|
|
| Unaffected by human:
IL-1alpha / IL-1RA / IL-2 / IL-3 / IL-4 / IL-6 / IL-7 / IL-8 /
TNF-alpha / IFN-alpha / IFN-gamma / mouse IL-1beta |
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| mouse |
| mouse IL-1beta |
100% |
|
|
| Unaffected by mouse:
IL-1alpha / IL-3 / IL-4 / IL-5 / IL-7 / IL-10 / TNF-alpha /
IFN-gamma / GM-CSF / human IL-1beta
|
| Compound |
Cross Reactivity |
Compound |
Cross Reactivity |
| rat |
| rat IL-1beta |
100% |
|
|
| Unaffected by rat:
IFN-gamma / IL-1alpha / IL-2 / IL-4 / IL-6 / IL-10 / MCP-1 /
MIP-1alpha / RANTES / human IL-1beta / mouse IL-1beta / pig
IL-1beta |
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45