|
|
 |
| Hydrogen
Peroxide Colorimetric Detection Kit |
| Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
907-015 |
|
|
|
|
| Hydrogen
Peroxide Chemiluminescent Detection Kit |
| Format |
Catalog # |
Kit Insert |
MSDS |
Assay Layout |
Citations |
| 96 Well Kit |
907-012 |
|
|
|
|
|
|
FOR RESEARCH PURPOSES ONLY, NOT FOR DIAGNOSTIC USE. |
 
Simple Microtiter Plate Method
Rapid Results: 5 Second Count Time
Measure up to 41 Samples in duplicate
Wide, Dynamic Range
|
Hydrogen peroxide (H2O2) is a reactive oxygen
metabolic by-product that serves as a key regulator for a number of
oxidative stress-related states. Functioning through NF kappa-§ and
other factors, hydroperoxide-mediated pathways have been linked to
asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number
of neurodegenerative diseases, and Down's syndrome. Perhaps the most
intriguing aspect of H2O2 biology is the recent
report that antibodies have the capacity to convert molecular oxygen
into hydrogen peroxide to contribute to the normal recognition and
destruction processes of the immune system. Measurement of this reactive
species will help to determine how oxidative stress modulates varied
intracellular pathways.
|
|
|
1. Pipet Standards and Samples in duplicate into the wells.
2. Add Substrate.
3. Place plate on a plate shaker for approximately 10 seconds to gently
mix.
4. Read in a plate luminometer with no delay between trigger and
reading, and a 5 second read time.
5. Calculate H2O2 concentrations from Standard
Curve.
|
 
| |
Chemiluminescent Hydrogen Peroxide (907-012) |
Colorimetric
Hydrogen Peroxide (907-015) |
| FORMAT |
in Buffer
|
in Tissue Culture
Media |
Colorimetric
|
| RANGE |
2.44-2,500 ng/mL
|
39.06-10,000 ng/mL
|
106.25-3,400 ng/mL
|
| SAMPLE SIZE |
50 µL |
50 µL |
50 µL |
| SENSITIVITY |
2.39 ng/mL
|
11.91 ng/mL
|
51.25 ng/mL
|
| PRECISION |
6.1-8.4% Inter Assay
5.8-9.6% Intra Assay |
3.9-8.9% Inter Assay
3.4-4.0% Intra Assay |
1.7-5.7% Inter Assay
3.0-10.0% Intra Assay |
SAMPLE TYPE
recommended dilution |
Data in Process
|
Data in Process
|
Horse heparin Plasma ( 1:64)
Human Serum (1:64)
Human Urine (1:64)
Tissue Culture Media (None) |
| SPECIES
SPECIFICITY |
non-specific
|
non-specific
|
non-specific
|
|
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45